{"id":59255,"date":"2018-03-09T22:47:06","date_gmt":"2018-03-09T22:47:06","guid":{"rendered":""},"modified":"2018-03-09T22:47:06","modified_gmt":"2018-03-09T22:47:06","slug":"regulation-of-the-11beta-hydroxysteroid-dehydrogenasetype-2-promoter-by-steroid-hormones-in-breast-cancer-cells-convergence-of-progesterone-receptor-binding-to-dna-and-jak-stat-pathway-activation","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/regulation-of-the-11beta-hydroxysteroid-dehydrogenasetype-2-promoter-by-steroid-hormones-in-breast-cancer-cells-convergence-of-progesterone-receptor-binding-to-dna-and-jak-stat-pathway-activation\/","title":{"rendered":"Regulation of the 11beta-hydroxysteroid dehydrogenasetype 2 promoter by steroid hormones in breast cancer cells. convergence of progesterone receptor binding to dna and jak\/stat pathway activation"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Alicia Subtil Rodr\u00edguez <\/strong><\/h2>\n<p>Steroid hormone receptors regulate gene expression interacting with target dna sequences but also activating cytoplasmic signalling pathways. Using the human 11beta-hydroxysteroid dehydrogenase type 2 (11 -hsd2) gene as a model, we investigated the contribution of both effects on a human progesterone responsive promoter in breast cancer cells. Our results suggest two different mechanisms of hormone-induced pr recruitment to the 11 -hsd2 promoter: i,) direct pr binding to dna at the proximal promoter, abolished when pr contains a mutated dna binding domain (dbd); and ii,) stat5a-mediated recruitment of pr to the distal promoter region, impaired by a jak\/stat pathway inhibitor. Therefore, coordination of pr transcriptional effects and cytoplasmic signalling activation, in particular jak\/stat pathway, are critical to regulate progestins-induced endogenous 11 -hsd2 gene expression in breast cancer cells. This is not unique to this promoter, as the jak\/stat inhibitor also alters expression of other progesterone-regulated genes. Additionally, we report rna polymerase ii tracking from the distal region upon pr and stat5a binding and we propose that this region works as hormone-dependent transcriptional enhancer.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulation of the 11beta-hydroxysteroid dehydrogenasetype 2 promoter by steroid hormones in breast cancer cells. convergence of progesterone receptor binding to dna and jak\/stat pathway activation<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulation of the 11beta-hydroxysteroid dehydrogenasetype 2 promoter by steroid hormones in breast cancer cells. convergence of progesterone receptor binding to dna and jak\/stat pathway activation <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Alicia Subtil Rodr\u00edguez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pompeu fabra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 22\/06\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Albert Jordan Vall\u00e9s<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ana Aranda iriarte <\/li>\n<li>jordi Bernu\u00e9s mart\u00ednez (vocal)<\/li>\n<li>luciano Di croce (vocal)<\/li>\n<li> Fern\u00e1ndez de mattos s\u00edlvia (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Alicia Subtil Rodr\u00edguez Steroid hormone receptors regulate gene expression interacting with target dna sequences but also activating 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