{"id":59368,"date":"2018-03-09T22:47:13","date_gmt":"2018-03-09T22:47:13","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/interacciones-moleculares-del-regulador-xyls-y-la-arn-polimerasacon-el-promotor-pm-del-plasmido-tol-pww0-de-pseudomonas-putida\/"},"modified":"2018-03-09T22:47:13","modified_gmt":"2018-03-09T22:47:13","slug":"interacciones-moleculares-del-regulador-xyls-y-la-arn-polimerasacon-el-promotor-pm-del-plasmido-tol-pww0-de-pseudomonas-putida","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/interacciones-moleculares-del-regulador-xyls-y-la-arn-polimerasacon-el-promotor-pm-del-plasmido-tol-pww0-de-pseudomonas-putida\/","title":{"rendered":"Interacciones moleculares del regulador xyls y la arn polimerasacon el promotor pm del plasmido tol pww0 de pseudomonas putida."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Patricia Dominguez Cuevas <\/strong><\/h2>\n<p>La transcripci\u00f3n constituye el primer paso en el proceso de expresi\u00f3n g\u00e9nica y uno de los puntos principales sujetos a regulaci\u00f3n. La ruta meta del pl\u00e1smido tol pww0 de pseudomomas putida codifica los enzimas necesarios para el catabolismo de alquilbenzoatos. El regulador transcripcional xyls, que pertenece a la familia arac, activa la transcripci\u00f3n desde el promotor del oper\u00f3n meta en respuesta a la presencia de benzoato y derivados.   a lo largo de este trabajo de tesis doctoral se han estudiado diferentes aspectos tanto globales como espec\u00edficos de la regulaci\u00f3n de la transcripci\u00f3n desde el promotor de clase ii, pm, del oper\u00f3n meta por la prote\u00edna reguladora xyls. En trabajos anteriores se hab\u00edan definido las secuencias en el promotor pm reconocidas por el regulador xyls y la dependencia de dicho promotor por dos arn polimerasas alternativas, asociadas a los factores  32 y  38, que mantienen la transcripci\u00f3n del oper\u00f3n meta a lo largo de la curva de crecimiento. Nuestros resultados nos han permitido definir las secuencia de nucle\u00f3tidos en la regi\u00f3n -10 que permite el reconocimiento por dichas arn polimerasas alternativas. El promotor pm parece estar dise\u00f1ado para responder a la respuesta de estr\u00e9s que provoca la presencia de compuestos arom\u00e1ticos. Hemos identificado los amino\u00e1cidos del la prote\u00edna xyls que participan en el reconocimiento espec\u00edfico de las dos repeticiones de secuencia en el promotor pm, lo que nos ha llevado a orientar la prote\u00edna en el promotor. La conformaci\u00f3n cabeza-cola que adoptan los dos mon\u00f3meros de xyls define la zona de contacto con la arn polimerasa, que probablemente juega un papel importante en la activaci\u00f3n de la transcripci\u00f3n. La uni\u00f3n secuencial y cooperativa de dos mon\u00f3meros de la prote\u00edna xyls al promotor, provoca una curvatura gradual del adn, que favorece los contactos con la arn polimerasa y el inicio de la transcripci\u00f3n. Por \u00faltimo, el estudio del papel del 3-metilbenzoato (3mb) en la activac<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Interacciones moleculares del regulador xyls y la arn polimerasacon el promotor pm del plasmido tol pww0 de pseudomonas putida.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Interacciones moleculares del regulador xyls y la arn polimerasacon el promotor pm del plasmido tol pww0 de pseudomonas putida. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Patricia Dominguez Cuevas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/06\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Silvia Marques Martin<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: manuel Espinosa padr\u00f3n <\/li>\n<li>carmen Michan dona (vocal)<\/li>\n<li>eduardo Santero saturnino (vocal)<\/li>\n<li>victoria Shingler (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Patricia Dominguez Cuevas La transcripci\u00f3n constituye el primer paso en el proceso de expresi\u00f3n g\u00e9nica y uno [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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