{"id":59647,"date":"2007-04-07T00:00:00","date_gmt":"2007-04-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/tratamiento-de-la-hepatitis-b-cronica-mediante-adenovirus-de-alta-capacidad-que-expresan-de-forma-inducible-el-gen-de-la-interleukina-12\/"},"modified":"2007-04-07T00:00:00","modified_gmt":"2007-04-07T00:00:00","slug":"tratamiento-de-la-hepatitis-b-cronica-mediante-adenovirus-de-alta-capacidad-que-expresan-de-forma-inducible-el-gen-de-la-interleukina-12","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/virologia\/tratamiento-de-la-hepatitis-b-cronica-mediante-adenovirus-de-alta-capacidad-que-expresan-de-forma-inducible-el-gen-de-la-interleukina-12\/","title":{"rendered":"Tratamiento de la hepatitis b cr\u00f3nica mediante adenovirus de alta capacidad que expresan de forma inducible el gen de la interleukina 12"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Julien Swen Crettaz <\/strong><\/h2>\n<p>Tratamiento de la hepatitis b cr\u00f3nica mediante adenovirus de alta capacidad que expresan de forma inducible el gen de la interleukina 12 #resumen: chronic hepatitis b treatment using a high-capacity adenovirus expressing interleukin 12 in an inducible and liver-specific manner. Il-12 induction has been shown to be a key determinant of viral clearance in chronic hepatitis b virus (hbv) infection. We tested the therapeutic potential of il-12 gene therapy in woodchucks with chronic whv infection, a condition that mi mi es dosel y chronic hepatitis b. For the genetic delivery of il-12 to woodchuck livers we have used a high-capacity adenovirus (hc-ad) encoding il-12 under the control of a hepato-speciric inducible system. The major drawbacks for the el i ni cal use of hc-ad reside in the difficulties for large-scale production and the absence of standardized methods for production and titration. In this regard, the first step of our work was to compare the different methods used by different groups to characterize hc-ad. From these studies we selected the quantitative pcr assay to monitor the production and titration of hc-ad infectious particles as well as for the estimation of contamination from other virus. A second major limitation for the el i ni cal appli cati\u00f3n of these vectors is the inflammatory reaction associated with systemic administration of high doses of virus. In this we showed that changing the route of administration of the adenovirus we reduced the toxicity of the vector. Administration of the adenovirus by direct injection i nto the liver parenchyma increases the transduction efficieney and reduced the inflammatory response by avoiding the action of kupffer cells. Then, we treated whv-chronically infected woodchucks with the therapeutic vector expressing il-12. Anim\u00e1is with relatively low basal viremias, but similar to the higher viremias found in hbv patients, showed a strong antiviral effect associated with the induction of a virus-specific immune response and low toxicity. Anim\u00e1is with high viral load did not respond to the treament. Furthermore, responder anim\u00e1is showed an improvement on liver histology and a reduction in the lev\u00e9is of regulatory t cellsdireccion:<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Tratamiento de la hepatitis b cr\u00f3nica mediante adenovirus de alta capacidad que expresan de forma inducible el gen de la interleukina 12<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Tratamiento de la hepatitis b cr\u00f3nica mediante adenovirus de alta capacidad que expresan de forma inducible el gen de la interleukina 12 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Julien Swen Crettaz <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/07\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gloria Gonzales Aseguinolaza<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: m. asuncion Buti ferret <\/li>\n<li>Miguel Chillon rodriguez (vocal)<\/li>\n<li>Francisco Borras cuesta (vocal)<\/li>\n<li>cristina Fillat fonts (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Julien Swen Crettaz Tratamiento de la hepatitis b cr\u00f3nica mediante adenovirus de alta capacidad que expresan de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,1085,1737,17749,280],"tags":[43040,18184,116473,131762,131763,53778],"class_list":["post-59647","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-enfermedades-infecciosas","category-inmunologia","category-navarra","category-virologia","tag-cristina-fillat-fonts","tag-francisco-borras-cuesta","tag-gloria-gonzales-aseguinolaza","tag-julien-swen-crettaz","tag-m-asuncion-buti-ferret","tag-miguel-chillon-rodriguez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/59647","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=59647"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/59647\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=59647"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=59647"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=59647"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}