{"id":59730,"date":"2007-06-07T00:00:00","date_gmt":"2007-06-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/dna-gyrase-a-model-peptides-as-potential-probes-to-evaluate-the-antibacterial-activity-of-quinolones-desing-synthesis-and-interaction-studies\/"},"modified":"2007-06-07T00:00:00","modified_gmt":"2007-06-07T00:00:00","slug":"dna-gyrase-a-model-peptides-as-potential-probes-to-evaluate-the-antibacterial-activity-of-quinolones-desing-synthesis-and-interaction-studies","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica-organica\/dna-gyrase-a-model-peptides-as-potential-probes-to-evaluate-the-antibacterial-activity-of-quinolones-desing-synthesis-and-interaction-studies\/","title":{"rendered":"Dna-gyrase a model peptides as potential probes to evaluate the antibacterial activity of quinolones: desing, synthesis and interaction studies"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mireia Gomez Piqueras <\/strong><\/h2>\n<p>Con la intenci\u00f3n de profundizar y entender mejor las interacciones que suceden a nivel molecular entre el adn, las quinolonas y el enzima girasa se han dise\u00f1ado y sintetizado diversos peptidos, modelos de girasa, que han sido utilizado como banco de prueba en el estudio por diversas t\u00e9cnicas biof\u00edsicas de las dichas interacciones. As\u00ed pues se han sintetizado en fase s\u00f3lida ocho peptidos que contienen los fragmentos 75-92 i 116-130 de la unidad gyra de la girasa de escherichia coli. Se han provocado diversos protocolos y se han introducido m\u00faltiples modificaciones con el objetivo de optimizar estos protocolos y mejorar los rendimientos globales. Aunque las secuencias demostraron su dificultad de s\u00edntesis, finalmente se consiguieron obtener los peptidos con buenos rendimientos (43%) estableciendo un protocolo de s\u00edntesis \u00f3ptimo. El comportamiento de cuatro de estos peptidos en presencia de adn ha sido estudiado por dicroismo molecular, determinando sus constantes de afinidad. Estos peptidos tambi\u00e9n han sido objeto de estudio estructural mediante la mencionada t\u00e9cnica. As\u00ed mismos se ha estudiado el complejo ternario formado por adn, peptido y ciprofloxacina determinado cualitativamente su interacci\u00f3n y validando el p\u00e9ptido como modelo del enzima natural para este tipo de estudios. La interacci\u00f3n entre ciprofloxacina y los peptidos se ha estudiado por espectroscopia de fluorescencia, determinando las constantes de afinidad. Al actividad de los peptidos frente a adn ha sido ensayada y comparada con la actividad del enzima natural. As\u00ed mismo se ha ensayado la actividad topoisomerasica de complejos formados por p\u00e9ptido y la subunidad gyrb con la intenci\u00f3n de evaluar los residuos eliminados respecto gyra. Los peptidos han demostrado no tener actividad topoisomerasica como era previsto y que no girasa por el adn. Aun as\u00ed, se ha demostrado su interacci\u00f3n con adn, interacci\u00f3n que es sensible a mutaciones en su secuencia. Mediante todas las t\u00e9cnicas biof<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Dna-gyrase a model peptides as potential probes to evaluate the antibacterial activity of quinolones: desing, synthesis and interaction studies<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Dna-gyrase a model peptides as potential probes to evaluate the antibacterial activity of quinolones: desing, synthesis and interaction studies <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mireia Gomez Piqueras <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/07\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Ernest Giralt Lledo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jordi Vila Estape <\/li>\n<li>Claudia Sissi (vocal)<\/li>\n<li>Karine Alvarez (vocal)<\/li>\n<li>Miriam Royo Exp\u00f3sito (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mireia Gomez Piqueras Con la intenci\u00f3n de profundizar y entender mejor las interacciones que suceden a nivel [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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