{"id":59930,"date":"2018-03-09T22:47:46","date_gmt":"2018-03-09T22:47:46","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-mecanismo-de-secrecion-de-la-interleuquina-1beta-de-dorada-sparus-aurata-l\/"},"modified":"2018-03-09T22:47:46","modified_gmt":"2018-03-09T22:47:46","slug":"caracterizacion-del-mecanismo-de-secrecion-de-la-interleuquina-1beta-de-dorada-sparus-aurata-l","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/caracterizacion-del-mecanismo-de-secrecion-de-la-interleuquina-1beta-de-dorada-sparus-aurata-l\/","title":{"rendered":"Caracterizaci\u00f3n del mecanismo de secreci\u00f3n de la interleuquina-1beta de dorada ( sparus aurata l.)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Gloria L\u00f3pez Castej\u00f3n <\/strong><\/h2>\n<p>Durante el desarrollo de este trabajo se han clonado y caracterizado tres mol\u00e9culas claves en el sistema de la interleuquina-1beta (il-1beta ) de dorada (sparus aurata l., Teleostei): el receptor p2x7, la caspasa-1 y el receptor tipo ii de la il-1beta (il-1rii). En primer lugar, se clon\u00f3 el receptor p2x7 de dorada y se compararon sus perfiles de actividad en respuesta a diferentes agonistas y antagonistas con los de los receptores p2x7 de rata, xenopus y pez cebra expresados en c\u00e9lulas hek, y tambi\u00e9n se estudi\u00f3 dicha respuesta en la l\u00ednea celular de fibroblastos de dorada saf-1. Esta informaci\u00f3n se us\u00f3 para investigar los mecanismos de liberaci\u00f3n de la il-1beta inducida por los receptores de mam\u00edfero y peces. A pesar de la exposici\u00f3n de fosfatidilserina en la cara externa de la membrana y la permeabilizaci\u00f3n celular observada en leucocitos de dorada tras la activaci\u00f3n con altas concentraciones de bzatp, la il-1beta  permanec\u00eda dentro de las c\u00e9lulas sin ser procesada. Sin embargo, la activaci\u00f3n del receptor p2x7 de rata expresado de forma ect\u00f3pica en c\u00e9lulas hek293 junto a la caspasa-1 humana produc\u00eda la liberaci\u00f3n de la forma precursora de la il-1beta  de dorada. Por el contrario, ni la activaci\u00f3n del receptor p2x7 de dorada ni del pez cebra induc\u00edan la secreci\u00f3n la il-1beta  ni de mam\u00edfero ni de peces cuando se expresaban en c\u00e9lulas hek293. Estos datos indicaban que la activaci\u00f3n de caspasa-1 mediada por el receptor p2x7 y la liberaci\u00f3n de la il-1beta  ocurren mediante diferentes v\u00edas de se\u00f1alizaci\u00f3n y sugieren que, aunque los mecanismos implicados en la secreci\u00f3n est\u00e9n conservados durante la evoluci\u00f3n, diferentes se\u00f1ales inflamatorias, han sido seleccionadas para la secreci\u00f3n de esta citoquina en diferentes vertebrados.  en segundo lugar, se clon\u00f3 la caspasa-1 de dorada, que presentaba un dominio conservado amino terminal card (dominio de reclutamiento de caspasa) y un dominio catal\u00edtico de caspasa carboxi-terminal. Se comprob<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n del mecanismo de secreci\u00f3n de la interleuquina-1beta de dorada ( sparus aurata l.)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n del mecanismo de secreci\u00f3n de la interleuquina-1beta de dorada ( sparus aurata l.) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Gloria L\u00f3pez Castej\u00f3n <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/07\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Victoriano Francisco Mulero Mendez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: cecilio Jes\u00fas Vidal moreno <\/li>\n<li>alfonsa Garcia ayala (vocal)<\/li>\n<li>f Wiergertjes geert (vocal)<\/li>\n<li>monserrat Elias arnanz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Gloria L\u00f3pez Castej\u00f3n Durante el desarrollo de este trabajo se han clonado y caracterizado tres mol\u00e9culas claves [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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