{"id":60399,"date":"2007-12-09T00:00:00","date_gmt":"2007-12-09T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-mutacional-del-extremo-c-terminal-de-la-a-13-galactosiltransferasa-bovina-estabilidad-y-catalisis\/"},"modified":"2007-12-09T00:00:00","modified_gmt":"2007-12-09T00:00:00","slug":"analisis-mutacional-del-extremo-c-terminal-de-la-a-13-galactosiltransferasa-bovina-estabilidad-y-catalisis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/analisis-mutacional-del-extremo-c-terminal-de-la-a-13-galactosiltransferasa-bovina-estabilidad-y-catalisis\/","title":{"rendered":"Analisis mutacional del extremo c terminal de la a-1,3-galactosiltransferasa bovina. estabilidad y catalisis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> JavierAntonino Linares Pasten <\/strong><\/h2>\n<p>La a-1,3-galactosiltransferasa (a3gt) est\u00e1 involucrada en la bios\u00edntesis de oligosac\u00e1ridos antig\u00e9nicos responsables del rechazo inmunol\u00f3gico hiperagudo (har) en el xenotransplante de \u00f3rganos de animales a humanos, por lo que esta enzima tiene un particular inter\u00e9s en biomedicina. Por otra parte, la a3gt constituye un modelo enzim\u00e1tico para estudios mecan\u00edsticos y estructurales de glicosiltransferasas. La enzima transfiere galactosa (gal) del udp-gal a la n-acetillactosamina (lacnac), o a la lactosa (lac). actualmente, los estudios cristalogr\u00e1ficos han mostrado dos tipos de estructuras con diferentes conformaciones en la zona c-terminal (aa.358-368). En la forma i, esta zona est\u00e1 altamente desordenada, pero en la forma ii est\u00e1 bien definida, lo cual sugiere que funciona como tapa que cierra el sitio catal\u00edtico. Estos cambios conformacioanles sugieren que el extremo c-terminal podr\u00eda tener un rol clave en la actividad catal\u00edtica. en la presente tesis doctoral se abord\u00f3 el estudio estructural\/funcional del extremo c-terminal desde un enfoque de an\u00e1lisis cin\u00e9tico y de estabilidad de mutantes de alanina de cada uno de los amino\u00e1cidos de las posiciones 358-368. De esta manera, la comparaci\u00f3n entre eficiencia catal\u00edtica y estabilidad enzim\u00e1tica proporcionan un m\u00e9todo que permite identificar los amino\u00e1cidos que tienen un rol importante en la uni\u00f3n de ligando o en el empaquetamiento estructural. para los mutantes k359a, y361am v363a y r365a no se ha detectado actividad, por lo cual, estas posiciones son fundamentales para la actividad a3gt. K359, y361 y r365 interaccionan con los fosfatos del udp-gal, adem\u00e1s, k359a tambien interaccionan con el hidroxilo 3 de la lactosa seg\u00fan modelizaci\u00f3n molecular, por lo cual se ha propuesto un rol de \u00e1cido\/base general a la lisina 359. Por otra parte, los mutantes n367a y v368a resultaron ser de 2 a 3 veces m\u00e1s activos que wt, respectivamente. los estudios de estabilidad revelan que wt y mutantes de residu<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Analisis mutacional del extremo c terminal de la a-1,3-galactosiltransferasa bovina. estabilidad y catalisis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Analisis mutacional del extremo c terminal de la a-1,3-galactosiltransferasa bovina. estabilidad y catalisis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 JavierAntonino Linares Pasten <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Ram\u00f3n llull<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/09\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Planas Sauter<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: llu\u00eds Comellas riera <\/li>\n<li>Carlos Semino (vocal)<\/li>\n<li>francesc Canals (vocal)<\/li>\n<li>gemma Arsequell ruiz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de JavierAntonino Linares Pasten La a-1,3-galactosiltransferasa (a3gt) est\u00e1 involucrada en la bios\u00edntesis de oligosac\u00e1ridos antig\u00e9nicos responsables del rechazo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[115,955,940,3178,32959],"tags":[32768,133384,133385,76114,133383,14845],"class_list":["post-60399","post","type-post","status-publish","format-standard","hentry","category-bioquimica-molecular","category-enzimologia","category-genetica-bioquimica","category-proteinas","category-ramon-llull","tag-antonio-planas-sauter","tag-carlos-semino","tag-francesc-canals","tag-gemma-arsequell-ruiz","tag-javierantonino-linares-pasten","tag-lluis-comellas-riera"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/60399","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=60399"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/60399\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=60399"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=60399"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=60399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}