{"id":60683,"date":"2018-03-09T22:48:29","date_gmt":"2018-03-09T22:48:29","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/kinetic-studies-of-a-xyloglucan-endotransglycosylase-a-key-enzime-in-plant-cell-morphogenesis\/"},"modified":"2018-03-09T22:48:29","modified_gmt":"2018-03-09T22:48:29","slug":"kinetic-studies-of-a-xyloglucan-endotransglycosylase-a-key-enzime-in-plant-cell-morphogenesis","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/enzimologia\/kinetic-studies-of-a-xyloglucan-endotransglycosylase-a-key-enzime-in-plant-cell-morphogenesis\/","title":{"rendered":"Kinetic studies of a xyloglucan endotransglycosylase, a key enzime in plant cell morphogenesis"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marc Saura Valls <\/strong><\/h2>\n<p>El presente trabajo de investigaci\u00f3n se enmarca en un proyecto europeo llamado e.D.E.N. (Enzyme discovery in hybrid aspen for fibre engineering, qlk5-ct-2001-00443), el objetivo del cual es la identificaci\u00f3n de nuevos enzimas vegetales para entender con mayor profundidad los procesos de formaci\u00f3n y modificaci\u00f3n de las fibras vegetales para abordar en el futuro la mejora de los par\u00e1metros de calidad de estas fibras, mediante la generaci\u00f3n de l\u00edneas transg\u00e9nicas de plantas. En el presente proyecto se pretende profundizar en el conocimiento de las xiloglucano endotransglicosilasas (xet), enzimas claves en la construcci\u00f3n y modificaci\u00f3n controlada de la red de xiloglucano-celulosa, estudiando su mecanismo de acci\u00f3n y su especificidad por sustrato. En este trabajo se estudia una xet de populus tremula x tremuloides, concretamente la xet16a (ptt-xet16a).  se dise\u00f1a y se valida un nuevo ensayo enzim\u00e1tico mediante electroforesis capilar (hpce), que permite el estudio cin\u00e9tico de las xet, utilizando oligosac\u00e1ridos de xiloglucano de bajo peso molecular y de estructura conocida como sustratos. Estos sustratos han estado sintetizados en el presente trabajo y tambi\u00e9n por el equipo del dr. Driguez en el cermav-cnrs.  se determina que el m\u00e1ximo de actividad de la ptt-xet16a se da entre ph 5 y 5.5 y entre 30 y 40 \u00c2\u00bac. Se demuestra que este enzima act\u00faa mediante un mecanismo cin\u00e9tico bi-bi ping-pong, en el que el aceptor act\u00faa como inhibidor competitivo del dador uni\u00e9ndose al enzima libre y en el que, dependiendo del dador utilizado , \u00e9ste tambi\u00e9n puede actuar como inhibidor competitivo del aceptor uni\u00e9ndose en los subsitios positivos del intermedio glicosilo-enzima y dando diferentes reacciones secundarias como son la polimerizaci\u00f3n del dador o la elongaci\u00f3n del producto, solamente si el dador presenta un grupo glucosilo en el extremo no reductor.  se eval\u00faa una librer\u00eda de xilogluco-oligosac\u00e1ridos sintetizada por el equipo del dr. Driguez en el cerm<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Kinetic studies of a xyloglucan endotransglycosylase, a key enzime in plant cell morphogenesis<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Kinetic studies of a xyloglucan endotransglycosylase, a key enzime in plant cell morphogenesis <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marc Saura Valls <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Ram\u00f3n llull<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 28\/09\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Planas Sauter<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Santiago Nonell marrugat <\/li>\n<li>Manuel Martin lomas (vocal)<\/li>\n<li>hugues Driguez (vocal)<\/li>\n<li>joan carles Ferrer artigas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marc Saura Valls El presente trabajo de investigaci\u00f3n se enmarca en un proyecto europeo llamado e.D.E.N. (Enzyme [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[955,13696,3209,32959],"tags":[32768,134027,101106,9300,134026,39695],"class_list":["post-60683","post","type-post","status-publish","format-standard","hentry","category-enzimologia","category-glucidos","category-quimica-de-los-hidratos-de-carbono","category-ramon-llull","tag-antonio-planas-sauter","tag-hugues-driguez","tag-joan-carles-ferrer-artigas","tag-manuel-martin-lomas","tag-marc-saura-valls","tag-santiago-nonell-marrugat"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/60683","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=60683"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/60683\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=60683"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=60683"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=60683"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}