{"id":60760,"date":"2007-04-10T00:00:00","date_gmt":"2007-04-10T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-las-modificaciones-post-traduccionales-de-nrta-subunidad-periplasmica-del-sistema-de-transporte-de-nitrato-nitrito-en-phormidium-laminosum\/"},"modified":"2007-04-10T00:00:00","modified_gmt":"2007-04-10T00:00:00","slug":"estudio-de-las-modificaciones-post-traduccionales-de-nrta-subunidad-periplasmica-del-sistema-de-transporte-de-nitrato-nitrito-en-phormidium-laminosum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/estudio-de-las-modificaciones-post-traduccionales-de-nrta-subunidad-periplasmica-del-sistema-de-transporte-de-nitrato-nitrito-en-phormidium-laminosum\/","title":{"rendered":"Estudio de las modificaciones post-traduccionales de nrta, subunidad periplasmica del sistema de transporte de nitrato\/nitrito en phormidium laminosum"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Javier Soria Esponera <\/strong><\/h2>\n<p>El an\u00e1lisis por electroforesis bidimensional (2-de) de membranas plasm\u00e1ticas de la cianobacteria phormidium laminosum cultivada con nitrato revela, despu\u00e9s de te\u00f1ir los geles con plata, m\u00e1s de 150 spots correspondientes a polip\u00e9ptidos diferentes. El posterior an\u00e1lisis por western blot de estos geles, utilizando anticuerpos policlonales de conejo, revelan la presencia de 6-7 isoformas de nrta, con la misma masa molecular (48 kda aprox.) Pero con una variaci\u00f3n en su punto isoel\u00e9ctrico de 0.10-0.15 unidades de ph. Al revelar por western blot usando anticuerpos anti fosfo-tirosina, aparecen spots que coinciden en pi y masa molecular con los correspondientes a la prote\u00edna nativa, lo que indica que la prote\u00edna nativa est\u00e1 fosforilada. En este trabajo se estudia el efecto de dicha fosforilaci\u00f3n en la regulaci\u00f3n de la asimilaci\u00f3n del nitr\u00f3geno utilizando una prote\u00edna mutante de sustituci\u00f3n de la tirosina fosforilable por una asparragina. los resultados muestran que la fosforilaci\u00f3n tiene una funci\u00f3n estabilizadora dificultando la desestructuraci\u00f3n tridimensional de la prote\u00edna. adem\u00e1s se ha conseguido purificar la prote\u00edna nrta nativa a partir de membranas plasm\u00e1ticas y se ha detectado en muestras de membranas la presencia de la prote\u00edna nrtd mediante espectrometr\u00eda de masas. Este resultado contrasta con la localizaci\u00f3n de la otra subunidad de hidr\u00f3lisis de atp del transportador nrt (nrtc), lo que podr\u00eda implicar que se nrtd sea una prote\u00edna de uni\u00f3n a membrana o que interaccione fuertemente con el componente transmembranal del transportador nrtb. En cualquiera de los casos, se demuestra un comportamiento diferente de nrtd y nrtc, lo que evidencia que su aportaci\u00f3n al proceso de transporte podr\u00eda ser distinto.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de las modificaciones post-traduccionales de nrta, subunidad periplasmica del sistema de transporte de nitrato\/nitrito en phormidium laminosum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de las modificaciones post-traduccionales de nrta, subunidad periplasmica del sistema de transporte de nitrato\/nitrito en phormidium laminosum <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Javier Soria Esponera <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 04\/10\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Luis Serra Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: vicente Rubio zamora <\/li>\n<li>Emilio Fernandez reyes (vocal)<\/li>\n<li>Jos\u00e9 Mar\u00eda Vega piqueres (vocal)<\/li>\n<li>asensio Gonzalez lartitegui (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Javier Soria Esponera El an\u00e1lisis por electroforesis bidimensional (2-de) de membranas plasm\u00e1ticas de la cianobacteria phormidium laminosum [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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