{"id":61174,"date":"2018-03-09T22:49:03","date_gmt":"2018-03-09T22:49:03","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/identificacion-de-secuencias-pepta%c2%addicas-especa%c2%adficas-para-celulas-de-cancer-de-prostata-metastatico-direccionamiento-de-vectores-adenovirales\/"},"modified":"2018-03-09T22:49:03","modified_gmt":"2018-03-09T22:49:03","slug":"identificacion-de-secuencias-pepta%c2%addicas-especa%c2%adficas-para-celulas-de-cancer-de-prostata-metastatico-direccionamiento-de-vectores-adenovirales","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/identificacion-de-secuencias-pepta%c2%addicas-especa%c2%adficas-para-celulas-de-cancer-de-prostata-metastatico-direccionamiento-de-vectores-adenovirales\/","title":{"rendered":"Identificaci\u00f3n de secu\u00e9ncias pept\u00eddicas espec\u00edficas para c\u00e9lulas de c\u00e1ncer de pr\u00f3stata metast\u00e1tico. direccionamiento de vectores adenovirales."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Anna Marazuela Duque <\/strong><\/h2>\n<p>El c\u00e1ncer de pr\u00f3stata es el m\u00e1s frecuente de los tumores diagnosticados en hombres adultos y la segunda causa de muerte despu\u00e9s del c\u00e1ncer de pulm\u00f3n. Las terapias convencionales son especialmente efectivas en las primeras etapas de la enfermedad siendo el desarrollo de met\u00e1stasis la principal causa de muerte de estos pacientes.  Por tanto, son necesarias nuevas terapias dirigidas espec\u00edficamente a las c\u00e9lulas metast\u00e1sicas y  por ello consideramos la terapia g\u00e9nica como una posible opci\u00f3n. Sin embargo, uno de los principales problemas de la terapia g\u00e9nica en este \u00e1mbito es la inespecificidad de la infecci\u00f3n, en particular de la infecci\u00f3n de c\u00e9lulas del hu\u00e9sped mediante los receptores virales coxsackievirus b and adenovirus receptor (car).  el objetivo de nuestro trabajo es identificar secuencias pept\u00eddicas espec\u00edficas para el reconocimiento de c\u00e9lulas metast\u00e1ticas y utilizarlas en el direccionamiento de vectores adenovirales con finalidad terap\u00e9utica.  mediante el cribado de una librer\u00eda de fagos (phage display ph.D.-C7c) hemos seleccionado tres secuencias pept\u00eddicas expresadas en los fagos: ctpqnttmc (fago ctpq), cypsrsplc (fago cyps)  y cplhqrpmc (fago cplh) que presentan respectivamente una eficiencia de uni\u00f3n in vitro a c\u00e9lulas metast\u00e1ticas de c\u00e1ncer de pr\u00f3stata (pc3mm2) de 2.6, 6 y 95 veces respecto al control (fago m13). Mediante mutag\u00e9nesis dirigida y recombinaci\u00f3n hom\u00f3loga hemos introducido estas secuencias en el hi-loop de la fibra del adenovirus 5 portador de los genes reporteros de luciferasa y prote\u00edna verde (gfp). Hemos obtenido 3 adenovirus recombinantes: adtlctpq (secuencia ctpqnttmc en el hi-loop), adtlcyps (secuencia cypsrsplc en el hi-loop) y adtlcplh (secuencia cplhqrpmc en el hi-loop) y comprobado su infectividad in vitro en c\u00e9lulas hek293 y en la l\u00ednea metast\u00e1tica de c\u00e1ncer de pr\u00f3stata humana pc3mm2, as\u00ed como en la linea parental pc3p. Los adenovirus recombinantes adtlctpq, adtlcyps y adtlcplh infectan de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Identificaci\u00f3n de secu\u00e9ncias pept\u00eddicas espec\u00edficas para c\u00e9lulas de c\u00e1ncer de pr\u00f3stata metast\u00e1tico. direccionamiento de vectores adenovirales.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Identificaci\u00f3n de secu\u00e9ncias pept\u00eddicas espec\u00edficas para c\u00e9lulas de c\u00e1ncer de pr\u00f3stata metast\u00e1tico. direccionamiento de vectores adenovirales. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Anna Marazuela Duque <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 31\/10\/2007<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Angels Fabra Fres<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Ramon Alemany Bonastre <\/li>\n<li>Cristina Fillat Fonts (vocal)<\/li>\n<li>Josep Mar\u00eda Aran Perramon (vocal)<\/li>\n<li>Jordi Barquinero Ma\u00f1ez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Anna Marazuela Duque El c\u00e1ncer de pr\u00f3stata es el m\u00e1s frecuente de los tumores diagnosticados en hombres [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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