{"id":62881,"date":"2018-03-09T22:50:47","date_gmt":"2018-03-09T22:50:47","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/optimizacion-de-la-expresion-del-gen-chy-del-procesamiento-y-de-la-secrecion-de-quimosina-bovina-en-aspergillus-awamori\/"},"modified":"2018-03-09T22:50:47","modified_gmt":"2018-03-09T22:50:47","slug":"optimizacion-de-la-expresion-del-gen-chy-del-procesamiento-y-de-la-secrecion-de-quimosina-bovina-en-aspergillus-awamori","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/optimizacion-de-la-expresion-del-gen-chy-del-procesamiento-y-de-la-secrecion-de-quimosina-bovina-en-aspergillus-awamori\/","title":{"rendered":"Optimizaci\u00f3n de la expresi\u00f3n del gen chy, del procesamiento y de la secreci\u00f3n de quimosina bovina en aspergillus awamori"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Martha Griselda Florez Rangel <\/strong><\/h2>\n<p>Los problemas de suministro de cuajo animal y la expansi\u00f3n de la industria del queso forzaron la b\u00fasqueda de enzimas alternativas al cuajo animal. Adem\u00e1s durante los \u00faltimos a\u00f1os la producci\u00f3n de prote\u00ednas heter\u00f3logas en hongos filamentosos ha sido dr\u00e1sticamente mejorada. Se realiz\u00f3 en nuestro laboratorio el dise\u00f1o y s\u00edntesis del gen chy para la expresi\u00f3n en aspergillus awamori. Para la obtenci\u00f3n de quimosina recombinante utilizando el gen sint\u00e9tico se han construido casetes de expresi\u00f3n utilizando las se\u00f1ales de expresi\u00f3n de los genes amyb de aspergillus oryzae y gla de aspergillus niger. En las construcciones se utilizar\u00f3n las se\u00f1ales l\u00edder f\u00fangicas correspondientes a los promotores utilizados y a su vez se realizaron construcciones utilizando la se\u00f1al pre propia del gen de la quimosina. Los transformantes obtenidos producen  quimosina recombinante en cantidades aceptables, destac\u00e1ndose la producci\u00f3n de los transformantes que utilizan las se\u00f1ales de expresi\u00f3n del gen amyb de aspergillus oryzae  con una producci\u00f3n de 23 mg\/l. El an\u00e1lisis del transcrito muestra que el gen se estaba transcribiendo correctamente y que posiblemente los limitantes en la producci\u00f3n se encontraban a nivel de secreci\u00f3n. El medio de producci\u00f3n utilizado para la obtenci\u00f3n de quimosina recombinante fue el medio mdfa modificado, suplementado con 1%  de case\u00edna, se observ\u00f3 que con este medio se ve favorecida la producci\u00f3n. Se observ\u00f3 que utilizando los p\u00e9ptidos de la case\u00edna como suplemento en el medio de producci\u00f3n de la quimosina recombinante se ve aumentado la secreci\u00f3n. Con la utilizaci\u00f3n de estrategias para la producci\u00f3n de prote\u00ednas heter\u00f3logas en hongos filamentosos como utilizaci\u00f3n del gen sint\u00e9tico, de se\u00f1ales de expresi\u00f3n fuertes y propias del hospedador o especies filogen\u00e9ticamente cercanas, y optimizaci\u00f3n del medio de cultivo, se ha logrado obtener quimosina recomb\u00ednate, observando que el factor limitante es el proceso de secreci\u00f3<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Optimizaci\u00f3n de la expresi\u00f3n del gen chy, del procesamiento y de la secreci\u00f3n de quimosina bovina en aspergillus awamori<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Optimizaci\u00f3n de la expresi\u00f3n del gen chy, del procesamiento y de la secreci\u00f3n de quimosina bovina en aspergillus awamori <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Martha Griselda Florez Rangel <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/02\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Francisco Mart\u00edn Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas Manuel Cantoral Fern\u00e1ndez <\/li>\n<li>Baltasar Mayo P\u00e9rez (vocal)<\/li>\n<li>Paloma Liras Pad\u00edn (vocal)<\/li>\n<li>Ana G\u00f3mez Rodr\u00edguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Martha Griselda Florez Rangel Los problemas de suministro de cuajo animal y la expansi\u00f3n de la industria [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,4430],"tags":[138847,25381,4500,4494,138846,4496],"class_list":["post-62881","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-leon","tag-ana-gomez-rodriguez","tag-baltasar-mayo-perez","tag-jesus-manuel-cantoral-fernandez","tag-juan-francisco-martin-martin","tag-martha-griselda-florez-rangel","tag-paloma-liras-padin"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/62881","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=62881"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/62881\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=62881"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=62881"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=62881"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}