{"id":64383,"date":"2008-08-05T00:00:00","date_gmt":"2008-08-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulation-of-recombinant-protein-solubility-and-conformational-quality-in-escherichia-coli\/"},"modified":"2008-08-05T00:00:00","modified_gmt":"2008-08-05T00:00:00","slug":"regulation-of-recombinant-protein-solubility-and-conformational-quality-in-escherichia-coli","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/regulation-of-recombinant-protein-solubility-and-conformational-quality-in-escherichia-coli\/","title":{"rendered":"Regulation of recombinant protein solubility and conformational quality in escherichia coli"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Garcia Fruitos <\/strong><\/h2>\n<p>La necesidad de conseguir procesos de producci\u00f3n de bajo coste y r\u00e1pidos, han conducido a un uso creciente de las bacterias como organismos productores, hecho que ha permitido a muchas industrias farmacol\u00f3gicas comercializar un amplio abanico de prote\u00ednas recombinantes de inter\u00e9s. La producci\u00f3n de estas prote\u00ednas recombinantes en bacterias a menudo conduce a la formaci\u00f3n, fruto de un proceso secuencia-espec\u00edfico, de unos agregados insolubles llamados cuerpos de inclusi\u00f3n. Los cuerpos de inclusi\u00f3n son y han sido durante muchos a\u00f1os un fen\u00f3meno que ha generado preocupaci\u00f3n y se han descrito como estructuras refr\u00e1ctiles al microscopio \u00f3ptico, altamente hidratados, resistentes a algunos detergentes, cil\u00edndricos y de tama\u00f1o variable. Adem\u00e1s, hasta ahora, tambi\u00e9n ha prevalecido la idea que estos agregados est\u00e1n formados por prote\u00edna inactiva, un hecho que ha restringido de manera notable el espectro de prote\u00ednas que han sido finalmente comercializadas por la ind\u00fastria biotecnol\u00f3gica, ya que la forma inactiva no es \u00fatil. en mi trabajo de tesis he realizado un estudio detallado de la formaci\u00f3n de dichos cuerpos de inclusi\u00f3n, as\u00ed como de la prote\u00edna recombinante presente en la fracci\u00f3n soluble de las cepas productoras de \u00e9stos. Hemos podido ver que estos agregados presentan, contrariamente a lo que siempre se hab\u00eda cre\u00eddo, una actividad biol\u00f3gica considerable.  De hecho, los cuerpos de inclusi\u00f3n no s\u00f3lo presentan actividad, sino que adem\u00e1s la prote\u00edna activa presente en \u00e9stos se encuentra dispuesta siguiendo un patr\u00f3n caracter\u00edstico. Sumado a todo esto, fruto de nuestros resultados, podemos afirmar que los cuerpos de inclusi\u00f3n, generalmente ricos en prote\u00edna recombinante, son estructuras en las cuales coexisten perfectamente prote\u00ednas con estructura nativa, y por lo tanto activas, con prote\u00ednas mal plegadas que adoptan una disposici\u00f3n en forma de l\u00e1minas beta, caracter\u00edsticas de los agregados amiloideos. con el fin de explor<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulation of recombinant protein solubility and conformational quality in escherichia coli<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulation of recombinant protein solubility and conformational quality in escherichia coli <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Garcia Fruitos <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/05\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Villaverde Corrales<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jordi Barb\u00e9 Garc\u00eda <\/li>\n<li>pau Ferrer alegre (vocal)<\/li>\n<li>ursula Rinas (vocal)<\/li>\n<li>rosa Mar\u00eda Pint\u00f3 sol\u00e9 (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Garcia Fruitos La necesidad de conseguir procesos de producci\u00f3n de bajo coste y r\u00e1pidos, han conducido [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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