{"id":64533,"date":"2018-03-09T22:52:36","date_gmt":"2018-03-09T22:52:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-de-estrategias-bioseguras-para-la-obtencion-de-ca%c2%adtricos-transgenicos-eliminacion-de-genes-marcadores-y-transformacion-cloroplastica\/"},"modified":"2018-03-09T22:52:36","modified_gmt":"2018-03-09T22:52:36","slug":"desarrollo-de-estrategias-bioseguras-para-la-obtencion-de-ca%c2%adtricos-transgenicos-eliminacion-de-genes-marcadores-y-transformacion-cloroplastica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/desarrollo-de-estrategias-bioseguras-para-la-obtencion-de-ca%c2%adtricos-transgenicos-eliminacion-de-genes-marcadores-y-transformacion-cloroplastica\/","title":{"rendered":"Desarrollo de estrategias bioseguras para la obtenci\u00f3n de c\u00edtricos transg\u00e9nicos: eliminaci\u00f3n de genes marcadores y transformaci\u00f3n cloropl\u00e1stica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Alida Ballester De Matias <\/strong><\/h2>\n<p>T\u00edtulo: desarrollo de estrategias bioseguras para la obtenci\u00f3n de c\u00edtricos transg\u00e9nicos: eliminaci\u00f3n de genes marcadores y transformaci\u00f3n cloropl\u00e1stica.   la transformaci\u00f3n gen\u00e9tica se ha convertido en una tecnolog\u00eda fundamental tanto para la mejora gen\u00e9tica de plantas como para la investigaci\u00f3n b\u00e1sica sobre diversos aspectos de biolog\u00eda vegetal. En el caso de los c\u00edtricos, se han podido desarrollar procedimientos de transformaci\u00f3n nuclear para un elevado n\u00famero de genotipos. Sin embargo, todav\u00eda existen problemas tecnol\u00f3gicos importantes sobre los que resulta necesario avanzar. Entre ellos, la posibilidad de producir plantas libres de genes marcadores de selecci\u00f3n que confieren resistencia a antibi\u00f3ticos o herbicidas ser\u00eda de gran inter\u00e9s para vencer algunos de los obst\u00e1culos que plantea para la opini\u00f3n p\u00fablica el cultivo y la comercializaci\u00f3n de las plantas modificadas gen\u00e9ticamente. En la presente tesis se han investigado sistemas alternativos al uso del gen de la neomicina fosfotransferasa ii (nptii) como gen marcador de selecci\u00f3n en la transformaci\u00f3n gen\u00e9tica de c\u00edtricos: 1) selecci\u00f3n positiva no condicional mediante el gen de la isopentenil transferasa (ipt), 2) selecci\u00f3n positiva condicional mediante el sistema de la fosfomanosa isomerasa (pmi)\/manosa, 3) selecci\u00f3n de plantas transg\u00e9nicas basada en la expresi\u00f3n del gen delator de la ?-Glucuronidasa (uida), y 4) selecci\u00f3n por an\u00e1lisis pcr de los brotes transg\u00e9nicos regenerados. Por otra parte, se han establecido las condiciones y los medios de cultivo necesarios para abordar la transformaci\u00f3n y regeneraci\u00f3n de plantas transplast\u00f3micas de citrange carrizo y adem\u00e1s se ha dise\u00f1ado un vector de transformaci\u00f3n plastidial que incluye secuencias de recombinaci\u00f3n hom\u00f3loga espec\u00edficas del genoma cloropl\u00e1stico de c\u00edtricos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Desarrollo de estrategias bioseguras para la obtenci\u00f3n de c\u00edtricos transg\u00e9nicos: eliminaci\u00f3n de genes marcadores y transformaci\u00f3n cloropl\u00e1stica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo de estrategias bioseguras para la obtenci\u00f3n de c\u00edtricos transg\u00e9nicos: eliminaci\u00f3n de genes marcadores y transformaci\u00f3n cloropl\u00e1stica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Alida Ballester De Matias <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/05\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Leandro Pe\u00f1a Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Luis Navarro lucas <\/li>\n<li>vicente Moreno ferrero (vocal)<\/li>\n<li>carmen Fagoaga garcia (vocal)<\/li>\n<li>mercedes Dabauza mico (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Alida Ballester De Matias T\u00edtulo: desarrollo de estrategias bioseguras para la obtenci\u00f3n de c\u00edtricos transg\u00e9nicos: eliminaci\u00f3n de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[115,35,16820],"tags":[142389,142390,36927,12193,31243,31244],"class_list":["post-64533","post","type-post","status-publish","format-standard","hentry","category-bioquimica-molecular","category-ciencias-de-la-vida","category-politecnica-de-valencia","tag-alida-ballester-de-matias","tag-carmen-fagoaga-garcia","tag-leandro-pena-garcia","tag-luis-navarro-lucas","tag-mercedes-dabauza-mico","tag-vicente-moreno-ferrero"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/64533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=64533"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/64533\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=64533"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=64533"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=64533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}