{"id":64578,"date":"2018-03-09T22:52:36","date_gmt":"2018-03-09T22:52:36","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/dinamica-de-calcio-en-vesa%c2%adculas-de-secrecion-de-celulas-neuroendocrinas\/"},"modified":"2018-03-09T22:52:36","modified_gmt":"2018-03-09T22:52:36","slug":"dinamica-de-calcio-en-vesa%c2%adculas-de-secrecion-de-celulas-neuroendocrinas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/dinamica-de-calcio-en-vesa%c2%adculas-de-secrecion-de-celulas-neuroendocrinas\/","title":{"rendered":"Din\u00e1mica de calcio en ves\u00edculas de secreci\u00f3n de c\u00e9lulas neuroendocrinas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jaime Santo Domingo Mayoral <\/strong><\/h2>\n<p>Los granulos de secreci\u00f3n constituyen uno de los dep\u00f3sitos intracelulares de calcio menos conocidos, desde hace m\u00e1s de 20 a\u00f1os sabemos que contienen grandes cantidades de calcio total (unido m\u00e1s libre), sin embargo hasta la fecha existe cierta discusi\u00f3n acerca de cual es la concentraci\u00f3n de calcio libre en el interior (la mayor parte debe de encontrarse unido a un gran buffer interno formado fundamentalmente por prote\u00ednas). Adem\u00e1s los mecanismos moleculares que controlan tanto la captaci\u00f3n como la liberaci\u00f3n de calcio son poco conocidos y podr\u00edan ser distintos en diferentes tipos celulares. En definitiva, el significado fisiol\u00f3gico del calcio intragranular es todav\u00eda poco conocido. Nosotros hemos utilizado una aequorina quim\u00e9rica, producto de la fusi\u00f3n de una aequorina mutada (de baja afinidad por calcio) y la prote\u00edna vamp2 o sinaptobrevina, para monitorizar la din\u00e1mica de calcio en el interior de los gr\u00e1nulos de secreci\u00f3n de diferentes tipos celulares neuroendocrinos (c\u00e9lulas cromafines bovinas, c\u00e9lulas pc12 y c\u00e9lulas ins1). La concentraci\u00f3n de calcio libre en condiciones basales en el interior de las ves\u00edculas de secreci\u00f3n de celulas cromafines bovinas, teniendo en cuenta un ph intraluminal de 5,5, se sit\u00faa entre 50 y 100 micromolar, y entre 20 y 40 micromolar en las l\u00edneas celulares pc12 e ins1. En cuanto a los mecanismos de acumulaci\u00f3n de calcio en las ves\u00edculas de secreci\u00f3n, la exploraci\u00f3n farmacol\u00f3gica llevada a cabo con diferentes inhibidores de  atpasas de calcio (tapsigargina , bhq y \u00e1cido ciclopiaz\u00f3nico) sugiere la presencia de atpasas de calcio de tipo serca en la membrana de las ves\u00edculas de secreci\u00f3n. Por otro lado la disminuci\u00f3n en los niveles de calcio intravesiculares en presencia del inhibidor de la atpasa de protones vacuolar, bafilomicina y agentes que colapsan el gradiente  de protones, como el fccp y el cloruro amonico, sugiere la existencia de un intercambiador ca+\/h+ que opera a expensas del gradiente de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Din\u00e1mica de calcio en ves\u00edculas de secreci\u00f3n de c\u00e9lulas neuroendocrinas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Din\u00e1mica de calcio en ves\u00edculas de secreci\u00f3n de c\u00e9lulas neuroendocrinas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jaime Santo Domingo Mayoral <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Valladolid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/05\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Javier Alvarez Martin<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: ricardo Rigual bonastre <\/li>\n<li>manuela Garc\u00eda l\u00f3pez (vocal)<\/li>\n<li>ricardo Borges jurado (vocal)<\/li>\n<li>Mar\u00eda  almudena Albillos Martinez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jaime Santo Domingo Mayoral Los granulos de secreci\u00f3n constituyen uno de los dep\u00f3sitos intracelulares de calcio menos [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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