{"id":64994,"date":"2008-06-06T00:00:00","date_gmt":"2008-06-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/determinacion-de-la-actividad-enzimatica-poli-adp-ribosapolimerasa-en-celulas-del-sistema-inmune\/"},"modified":"2008-06-06T00:00:00","modified_gmt":"2008-06-06T00:00:00","slug":"determinacion-de-la-actividad-enzimatica-poli-adp-ribosapolimerasa-en-celulas-del-sistema-inmune","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/farmacologia\/determinacion-de-la-actividad-enzimatica-poli-adp-ribosapolimerasa-en-celulas-del-sistema-inmune\/","title":{"rendered":"Determinaci\u00f3n de la actividad enzim\u00e1tica poli-adp-ribosapolimerasa en c\u00e9lulas del sistema inmune"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Mart\u00ednez Mart\u00ednez M\u00aa Teresa <\/strong><\/h2>\n<p>Existen numerosas evidencias de que la enzima poli-adp-ribosa polimerasa 1 (parp-1) desempe\u00f1a un papel importante en la modulaci\u00f3n de la expresi\u00f3n g\u00e9nica durante el desarrollo yen respuesta a se\u00f1ales celulares espec\u00edficas, ejerciendo su acci\u00f3n a diferentes niveles. Parp-1 pertenece a una familia de enzimas (parp) que, usando nad+ como sustrato, sintetizan y transfieren homopol\u00edmeros de adp-ribosa en residuos de \u00e1cido glut\u00e1mico en prote\u00ednas aceptoras principalmente involucradas en la estabilidad de la cromatina yen el metabolismo de dna. La actividad de parp-1, responsable de casi el 90% de la poli-adp-ribosilaci\u00f3n de otras prote\u00ednas por asociaci\u00f3n f\u00edsica con prote\u00ednas relevantes como ios factores de transcripci\u00f3n. en este trabajo nos hemos centrado en poner a punto un m\u00e9todo de medida de la actividad poli-adp-ribosa polimerasa en c\u00e9lulas permeabilizadas utilizando -3h-nad+ como sustrato. Durante su caracterizaci\u00f3n, el m\u00e9todo ha demostrado tener una buena sensibilidad, precisi\u00f3n, especificidad y exactitud. Una vez optimizado, dicho m\u00e9todo se ha empleado en el estudio de las reacciones de poli-adp-ribosilaci\u00f3n mediadas por las enzimas parp en c\u00e9lulas del sistema inmune el estudio de la actividad parp en timocitos procedentes de ratones deficientes en parp-1 y de ratones parp-2-\/- frente a timocitos procedentes de ratones no modificados gen\u00e9ticamente pone de manifiesto que la actividad de poli-adp-ribosilaci\u00f3n mediada por parp-2 no seria responsable de la mayor susceptibilidad a apoptosis detectada en los timocitos de los ratones parp-2-\/-, aunque el incremento significativo de la actividad parp en ausencia de parp-2 si que podr\u00eda, de alguna forma, afectar al fenotipo observado. Dicho incremento de actividad nos sugiere tambi\u00e9n un efecto de parp-2 en una posible regulaci\u00f3n negativa de la actividad enzim\u00e1tica de parp-1. por otra parte los estudios de estimulaci\u00f3n de linfocitos t con pma+ionomicina y anticuerpos anti-c d3 solos y en com<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Determinaci\u00f3n de la actividad enzim\u00e1tica poli-adp-ribosapolimerasa en c\u00e9lulas del sistema inmune<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Determinaci\u00f3n de la actividad enzim\u00e1tica poli-adp-ribosapolimerasa en c\u00e9lulas del sistema inmune <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Mart\u00ednez Mart\u00ednez M\u00aa Teresa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Murcia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 06\/06\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Y\u00e9lamoa L\u00f3pez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas Hern\u00e1ndez cascales <\/li>\n<li>pablo Ram\u00edrez romero (vocal)<\/li>\n<li>Miguel Alcaraz ba\u00f1os (vocal)<\/li>\n<li>gonzalo Rubio pedraza (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mart\u00ednez Mart\u00ednez M\u00aa Teresa Existen numerosas evidencias de que la enzima poli-adp-ribosa polimerasa 1 (parp-1) desempe\u00f1a un [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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