{"id":66398,"date":"2018-03-09T22:54:43","date_gmt":"2018-03-09T22:54:43","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-de-un-multibiosensor-de-adn-para-diagnostico-temprano-de-cancer-de-mama\/"},"modified":"2018-03-09T22:54:43","modified_gmt":"2018-03-09T22:54:43","slug":"desarrollo-de-un-multibiosensor-de-adn-para-diagnostico-temprano-de-cancer-de-mama","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/desarrollo-de-un-multibiosensor-de-adn-para-diagnostico-temprano-de-cancer-de-mama\/","title":{"rendered":"Desarrollo de un multibiosensor de adn para diagn\u00f3stico temprano de c\u00e1ncer de mama"},"content":{"rendered":"

Tesis doctoral de Laura Garc\u00eda Carrascosa <\/strong><\/h2>\n

This thesis describes the development of a new methodology based on%&\/label-free biosensing to detect multiple mutations within a gene. As proof of%&\/concept it has been chosen the brca1 gene, which is related to early onset%&\/of inherited breast cancer.%&\/Two different biosensing technologies, nanomechanical and surface%&\/plasm\u00f3n resonance (spr) biosensors have been evaluated as an alternative%&\/tool to routine analytical methods for the detection of dna point mutations.%&\/For setting up an optimised biosensor method for this type of%&\/diagnostics, the following issues have been addressed:%&\/- the enhancement of dna immobilization onto gold surfaces at both biosensing%&\/platforms: it has been used the well-known method of thiol self assembly%&\/manolayers to immobilize dna sequences in a controlled%&\/way. Three different thiol groups have been evaluated to test dna%&\/linking to the gold surface in order to maximize chemisorption and%&\/minimize phisysorption phenomena, leading to highly dense dna%&\/receptor monolayers.%&\/- The enhancement of hybridization onto dna monolayers surfaces at both%&\/biosensing platforms: different strategies, as the use of lateral and%&\/vertical spacers, have been tested to control the self-assembly method%&\/and to improve target accessibility, leading to higher hybridization%&\/efficiency.%&\/- Evaluation of both biosensing platforms for dna detection: detection of 12%&\/and 25 mer dna sequences have been tried. Only spr biosensor%&\/were able to detect hybridization and to discriminate a single%&\/mismatch within a sequence. Nanomechanical biosensors that use a%&\/single microcantilever as transducer were unable to differentiate%&\/between a fully complementary sequence and a non-complementary%&\/one. For the nanomechanical biosensor a reference cantilever must%&\/be used in order to compensate other events not related to%&\/hybridization which could hide the detection of the specific%&\/hybridization. On the other hand, spr biosensors were able to detect%&\/12 mer and 25 complementary sequences with a 10 nm and 100 nm%&\/limit of detection, respectively. In addition, a clear discrimination of a%&\/single mismatch was demonstrated. Therefore this biosensing%&\/method was chosen for setting up a multianalyte label-free detection%&\/format.%&\/- Set-up of a multi-analyte detection format able to discriminate a single%&\/mismatched in pcr like products: a methodology of dna%&\/immobilization of multiple receptor sequences, based on the%&\/simultaneous co-inmobilization of two and four different sequences%&\/related to brca1 gene have been tried. Sequential detection of%&\/several pcr like target products using the same dna monolayer was%&\/demonstrated, addressing a detection limit at the nm range (50 nm).%&\/The main goal of this thesis has been the demonstration of the ability of%&\/the spr biosensor system for dna detection and discrimination of single%&\/mismatches through a multiplex format. The multiplex detection format has%&\/never been described before. The establishment of this methodology, as well as%&\/its ability to address a limit of detection in the nm range, sets a landmark in the%&\/direct and label-free detection of dna by biosensor devices. This allocates the%&\/biosensor technology as a competitive and complementary tool for dna%&\/analysis.<\/p>\n

 <\/p>\n

Datos acad\u00e9micos de la tesis doctoral \u00abDesarrollo de un multibiosensor de adn para diagn\u00f3stico temprano de c\u00e1ncer de mama<\/strong>\u00ab<\/h3>\n
    \n
  • T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo de un multibiosensor de adn para diagn\u00f3stico temprano de c\u00e1ncer de mama <\/li>\n
  • Autor:<\/strong>\u00a0 Laura Garc\u00eda Carrascosa <\/li>\n
  • Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n
  • Fecha de lectura de la tesis:<\/strong>\u00a0 17\/07\/2008<\/li>\n<\/ul>\n

     <\/p>\n

    Direcci\u00f3n y tribunal<\/h3>\n
      \n
    • Director de la tesis<\/strong>\n
        \n
      • Laura Lechuga Gomez<\/li>\n<\/ul>\n<\/li>\n
      • Tribunal<\/strong>\n
          \n
        • Presidente del tribunal: isabel Correas hornero <\/li>\n
        • c\u00e9sar Fern\u00e1ndez s\u00e1nchez (vocal)<\/li>\n
        • arben Merko\u00ed\u00a7i (vocal)<\/li>\n
        • Antonio Bernard miana (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          Tesis doctoral de Laura Garc\u00eda Carrascosa This thesis describes the development of a new methodology based on%&\/label-free biosensing to detect […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35],"tags":[48877,123017,118463,29889,146298,50657],"class_list":["post-66398","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","tag-antonio-bernard-miana","tag-arben-merkoii","tag-cesar-fernandez-sanchez","tag-isabel-correas-hornero","tag-laura-garcia-carrascosa","tag-laura-lechuga-gomez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/66398","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=66398"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/66398\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=66398"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=66398"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=66398"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}