{"id":66655,"date":"2018-03-09T22:55:00","date_gmt":"2018-03-09T22:55:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-funcional-de-las-fosfatasas-cdc-14-humanas-en-la-regulacion-del-ciclo-de-division-celular\/"},"modified":"2018-03-09T22:55:00","modified_gmt":"2018-03-09T22:55:00","slug":"caracterizacion-funcional-de-las-fosfatasas-cdc-14-humanas-en-la-regulacion-del-ciclo-de-division-celular","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/caracterizacion-funcional-de-las-fosfatasas-cdc-14-humanas-en-la-regulacion-del-ciclo-de-division-celular\/","title":{"rendered":"Caracterizaci\u00f3n funcional de las fosfatasas cdc 14 humanas en la regulaci\u00f3n del ciclo de divisi\u00f3n celular"},"content":{"rendered":"<h2>Tesis doctoral de <strong> M\u00aa Dolores V\u00e1zquez Novelle <\/strong><\/h2>\n<p>Las fosfatasas cdc14 desempe\u00f1an funciones relacionadas con la regulaci\u00f3n del ciclo celular en diversos organismos eucariotas, actuando como antagonistas de la actividad cdk (quinasa dependiente de ciclina). En las levaduras saccharomyces cerevisiae y schizosaccharomyces pombe, respectivamente, cdc14 y flp1\/clp1 participan en la regulaci\u00f3n de la salida de mitosis, promoviendo la inhibici\u00f3n de la actividad cdk mit\u00f3tica mediante mecanismos de acci\u00f3n diferentes. con el fin de estudiar las funciones de las prote\u00ednas cdc14 humanas, hcdc14a y hcdc14b, comenzamos analizando la homolog\u00eda funcional entre \u00e9stas y flp1. De este modo comprobamos que ambas prote\u00ednas humanas complementan el fenotipo correspondiente a la falta de flp1 en s. Pombe, mediante mecanismos diferentes. La fosfatasa hcdc14a, como flp1, desfosforila a cdc25 (activador de los complejos cdk mit\u00f3ticos) e induce as\u00ed la reducci\u00f3n de sus niveles proteicos; mientras que el mecanismo de la complementaci\u00f3n por hcdc14b no se conoce. a continuaci\u00f3n, determinamos que hcdc14a tambi\u00e9n es capaz de desfosforilar in vitro al menos a dos de las prote\u00ednas cdc25 humanas en residuos fosforilados por cdk1, a hcdc25a (en serina 115 y serina 320) y a hcdc25c (en residuos no determinados). Adem\u00e1s, probamos que la desfosforilaci\u00f3n de hcdc25a por hcdc14a en estos dos residuos identificados promueve su degradaci\u00f3n. por \u00faltimo, estudiamos los efectos que tienen la sobre-expresi\u00f3n y del silenciamiento de la expresi\u00f3n de hcdc14a sobre la progresi\u00f3n por mitosis en varias l\u00edneas celulares humanas. Determinamos as\u00ed que hcdc14a, de forma dependiente de su actividad fosfatasa, participa en la regulaci\u00f3n de los complejos cdk mit\u00f3ticos a la entrada en mitosis, a trav\u00e9s de la inhibici\u00f3n de la actividad catal\u00edtica de hcdc25a. Obtuvimos tambi\u00e9n datos que sugieren que hcdc14a podr\u00eda inducir la inactivaci\u00f3n de los complejos cdk mit\u00f3ticos a la salida de mitosis en parte, posiblemente, a trav\u00e9s de la desfosforilaci\u00f3n y consiguiente degradaci\u00f3n de hcdc25a.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n funcional de las fosfatasas cdc 14 humanas en la regulaci\u00f3n del ciclo de divisi\u00f3n celular<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n funcional de las fosfatasas cdc 14 humanas en la regulaci\u00f3n del ciclo de divisi\u00f3n celular <\/li>\n<li><strong>Autor:<\/strong>\u00a0 M\u00aa Dolores V\u00e1zquez Novelle <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Salamanca<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/07\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mar\u00eda Sacrist\u00e1n Mart\u00edn<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: dionisio Miguel Mart\u00edn zanca <\/li>\n<li>david Santamar\u00eda velilla (vocal)<\/li>\n<li>carme Gallego gonzalez (vocal)<\/li>\n<li>neus Agell jane (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de M\u00aa Dolores V\u00e1zquez Novelle Las fosfatasas cdc14 desempe\u00f1an funciones relacionadas con la regulaci\u00f3n del ciclo celular en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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