{"id":68050,"date":"2018-03-09T22:56:30","date_gmt":"2018-03-09T22:56:30","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/efectos-de-la-sobreexpresion-de-la-integrina-alphavbeta3-y-del-gen-supresor-p16ink4a-en-modelos-de-cancer-de-pancreas\/"},"modified":"2018-03-09T22:56:30","modified_gmt":"2018-03-09T22:56:30","slug":"efectos-de-la-sobreexpresion-de-la-integrina-alphavbeta3-y-del-gen-supresor-p16ink4a-en-modelos-de-cancer-de-pancreas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/efectos-de-la-sobreexpresion-de-la-integrina-alphavbeta3-y-del-gen-supresor-p16ink4a-en-modelos-de-cancer-de-pancreas\/","title":{"rendered":"Efectos de la sobreexpresi\u00f3n de la integrina alphavbeta3 y del gen supresor p16ink4a en modelos de c\u00e1ncer de p\u00e1ncreas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Sandra March\u00e1n Sancho <\/strong><\/h2>\n<p>El objetivo de esta tesis doctoral ha sido el estudio de los efectos de la sobreexpresi\u00f3n de la integrina \u00c2\u00bfv\u00edY3 del papel de la prote\u00edna p16 m\u00e1s all\u00e1 de su acci\u00f3n como cki, en modelos de c\u00e1ncer de p\u00e1ncreas humano. para ello se transfectaron con el cdna de la subunidad (33 dos l\u00edneas tumorales pancre\u00e1ticas (np-9 y np-18) y se obtuvieron l\u00edneas con expresi\u00f3n estable de esta integrina (np-9|33 y np-18(33). Una vez comprobada la correcta expresi\u00f3n y localizaci\u00f3n en membrana de la integrina avp3, se caracterizaron los efectos a nivel proliferativo y migratorio de su sobreexpresi\u00f3n in vitro e in vivo. Tanto np-9p3como np-18p3 no presentaban cambios en su capacidad de proliferaci\u00f3n in vitro ni en el perfil de ciclo celular respecto a las l\u00edneas parentales. Sin embargo, la sobreexpresi\u00f3n de (33 reduc\u00eda el crecimiento tumoral in vivo, tanto en modelos subcut\u00e1nos como intrapancre\u00e1ticos. Las l\u00edneas transfectadas con (33 presentaban una mayor capacidad de migraci\u00f3n respecto a las c\u00e9lulas parentales. una vez caracterizado el comportamiento de estas l\u00edneas, se iniciaron los estudios de los efectos de la reintroducci\u00f3n del gen p16 ink4a en estos modelos. La prote\u00edna p16 presentaba una localizaci\u00f3n tanto nuclear como citoplasm\u00e1tica, apreci\u00e1ndose un patr\u00f3n de tinci\u00f3n denso en zonas cercanas a ala membrana plasm\u00e1tica donde colocalizaba con talina y cdk6. Adem\u00e1s, la sobreexpresi\u00f3n de p16 provocaba cambios en la distribuci\u00f3n y forma de los contactos focales y disminu\u00eda los niveles totales de la subunidad (33 en las l\u00edneas np-9|33 y np-18|33. El tratamiento con un p\u00e9ptido mim\u00e9tico de p16 alteraba el patr\u00f3n de distribuci\u00f3n t\u00edpico de la integrina avp3en los contactos focales. Estos resultados abogan a favor de una posible regulaci\u00f3n cruzada de la expresi\u00f3n y\/o de la funci\u00f3n entre la prote\u00edna p16 y la integrina \u00c2\u00bfv\u00edY3 finalmente, la sobreexpresi\u00f3n de p16 reduc\u00eda la capacidad de migraci\u00f3n de las l\u00edneas tumorales pancre\u00e1ticas, esto correlacionaba con un aumento de la relaci\u00f3n rhoa\/rac1 en las l\u00edneas infectadas con ad-p16.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Efectos de la sobreexpresi\u00f3n de la integrina alphavbeta3 y del gen supresor p16ink4a en modelos de c\u00e1ncer de p\u00e1ncreas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Efectos de la sobreexpresi\u00f3n de la integrina alphavbeta3 y del gen supresor p16ink4a en modelos de c\u00e1ncer de p\u00e1ncreas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Sandra March\u00e1n Sancho <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/11\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Adela Mazo Sanchez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: mar\u00ed\u00a7al Pastor anglada <\/li>\n<li>eva c. Vaquero raya (vocal)<\/li>\n<li>carme Auladell costa (vocal)<\/li>\n<li>francesc Mitjans prat (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Sandra March\u00e1n Sancho El objetivo de esta tesis doctoral ha sido el estudio de los efectos de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[951,115,9399],"tags":[33982,37096,149540,53283,12645,149539],"class_list":["post-68050","post","type-post","status-publish","format-standard","hentry","category-barcelona","category-bioquimica-molecular","category-cultivo-celular","tag-adela-mazo-sanchez","tag-carme-auladell-costa","tag-eva-c-vaquero-raya","tag-francesc-mitjans-prat","tag-marial-pastor-anglada","tag-sandra-marchan-sancho"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/68050","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=68050"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/68050\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=68050"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=68050"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=68050"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}