{"id":68091,"date":"2018-03-09T22:56:33","date_gmt":"2018-03-09T22:56:33","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/multitrophic-interactions-involved-in-biological-control-of-evocado-white-root-rot-caused-by-rosellinia-necatrix\/"},"modified":"2018-03-09T22:56:33","modified_gmt":"2018-03-09T22:56:33","slug":"multitrophic-interactions-involved-in-biological-control-of-evocado-white-root-rot-caused-by-rosellinia-necatrix","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/multitrophic-interactions-involved-in-biological-control-of-evocado-white-root-rot-caused-by-rosellinia-necatrix\/","title":{"rendered":"Multitrophic interactions involved in biological control of evocado white root rot caused by rosellinia necatrix"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Clara Pliego Prieto <\/strong><\/h2>\n<p>Con el objetivo de identificar nuevos agentes bacterianos de biocontrol frente a la podredumbre blanca del aguacate causada por r. Necatrix, en esta tesis doctoral se desarroll\u00f3 un m\u00e9todo de selecci\u00f3n que permite el aislamiento de cepas bacterianas que colonizan eficientemente la rizosfera de plantas de aguacate. Tras utilizar raices de aguacate como material selectivo de cepas bacterianas capaces de establecerse en las mismas y migrar a los \u00e1pices radiculares durante su crecimiento, se aislaron 10 cepas colonizadoras de la raiz, y antagonistas in vitro frente a r. Necatrix. Tres aislados (p. Alcaligenes avo73, p. Putida avo102, p. Pseudoalcaligenes avo110), se seleccionaron por su baja resistencia a antibi\u00f3ticos bacterianos. de entre estos aislados, p. Pseudoalcaligenes avo110 y p. Alcaligenes avo73 mostraron mecanismos similares que podr\u00edan contribuir al control efectivo de la enfermedad; sin embargo, a pesar del mayor n\u00famero de actividades antagonistas mostradas por avo73, esta cepa no es capaz de reducir el desarrollo de la enfermedad a diferencia de avo110 que redujo el desarrollo de los s\u00edntomas en un 20%. El an\u00e1lisis a nivel microsc\u00f3pico de las interacciones de estos aislados con la ra\u00edz de aguacate y con las hifas de r. Necatrix, revel\u00f3 que la cepa con capacidad de biocontrol avo110, colonia eficientemente las uniones entre c\u00e9lulas epid\u00e9rmicas de la ra\u00edz y las hifas del pat\u00f3geno; sin embargo, avo73 se encontr\u00f3 fundamentalmente asociada a pelos radiculares y en uni\u00f3n d\u00e9bil con el hongo. Las diferentes interacciones bacteria, rizosfera y hongo filopat\u00f3geno mostradas parecen estar relacionadas con la diferente capacidad de biocontrol frente a r. Necatrix de estos aislados. para analizar el proceso de infecci\u00f3n de ra\u00edces de aguacatge por r. Necatrix se contruy\u00f3 un sector plasm\u00eddico pcphy1egfp. Las im\u00e1genes obtenidas mediante microscopia l\u00e1ser confocal muestran que la penetraci\u00f3n del hongo en la ra\u00edz ocurre a trav\u00e9s de las lenticelas y de las uniones formadas entre c\u00e9lulas de la epidermis. Las hifas se observaron por primera vez, atravesando las c\u00e9lulas de la epidermis, la corteza y los haces vasculares. Estos resultados muestran que la estrategia de colonizaci\u00f3n de ra\u00edz de aguacate llevada a cabo por avo110 se correlaciona con los sitios de la ra\u00edz que utiliza r. Necatrix para su penetraci\u00f3n, pudiendo suponer este patr\u00f3n de colonizaci\u00f3n una barrera f\u00edsica frente a la entrada del pat\u00f3geno. tras analizar a nivel microsc\u00f3pico las interacciones establecidas por avo110 con la ra\u00edz de aguacate y con las hifas del pat\u00f3geno, se inici\u00f3 un estudio dirigido a la identificaci\u00f3n de genes de esta bacteria implicados en la interacci\u00f3n con el hongo pat\u00f3geno utilizando la t\u00e9cnica signature tagged mutagenesis. Hasta la fecha, se han descrito 7 genes implicados en el metabolismo central, regulaci\u00f3n del mensajero secundario (c)-di-gmp y protecci\u00f3n de la bacteria frente a las defensas del hongo.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Multitrophic interactions involved in biological control of evocado white root rot caused by rosellinia necatrix<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Multitrophic interactions involved in biological control of evocado white root rot caused by rosellinia necatrix <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Clara Pliego Prieto <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 M\u00e1laga<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/11\/2008<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Cayo Juan Ramos Rodriguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: emilio Montesinos segui <\/li>\n<li>sandra de Weert (vocal)<\/li>\n<li>Manuel Espinosa urgel (vocal)<\/li>\n<li>satoko Kanematsu (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Clara Pliego Prieto Con el objetivo de identificar nuevos agentes bacterianos de biocontrol frente a la podredumbre [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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