{"id":69229,"date":"2004-09-07T00:00:00","date_gmt":"2004-09-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/produccion-inducible-de-lactococina-a-pediocina-pa-1-colicina-v-e-interleuquina-2-en-cepas-de-lactococcus-lactis-productoras-de-nisina\/"},"modified":"2004-09-07T00:00:00","modified_gmt":"2004-09-07T00:00:00","slug":"produccion-inducible-de-lactococina-a-pediocina-pa-1-colicina-v-e-interleuquina-2-en-cepas-de-lactococcus-lactis-productoras-de-nisina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/produccion-inducible-de-lactococina-a-pediocina-pa-1-colicina-v-e-interleuquina-2-en-cepas-de-lactococcus-lactis-productoras-de-nisina\/","title":{"rendered":"Producci\u00f3n inducible de lactococina a, pediocina pa-1, colicina v e interleuquina-2 en cepas de lactococcus lactis productoras de nisina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Antonio Fern\u00e1ndez Dumont <\/strong><\/h2>\n<p>La posibilidad de intercambiar tanto los l\u00edderes como los sistemas de secreci\u00f3n y procesado que intervienen en la bios\u00edntesis de diversas bacteriocinas puede ser explotada para conseguir la producci\u00f3n heter\u00f3loga de estos p\u00e9ptidos antimicrobianos.En este trabajo se ha conseguido la producci\u00f3n de las bacteriocinas lactococina a,pedicina pa-1 y colicina v en lactococcus lactis.Adem\u00e1s , se ha conseguido la producci\u00f3n de una prote\u00edna eucariota, la citoquina interleuquina-2 en esa misma especie.Para ello , ha sido necesaria la introducci\u00f3n de tres genes:(a) un gen estructural h\u00edbrido con la fusi\u00f3n , en un mismo marco de lectura , de las secuencias que codifican el l\u00edder de la lactococina a y el prop\u00e9ptido de inter\u00e9s(prolactococina a,propediocina pa-1,procolicina v o proninterleuquina-2)y (b) los genes icnc y lcnd,que codifican elsistema de secreci\u00f3n y procesado de la lactococina a.  los genes estructurales h\u00edbridos se construyeron de tal modo que su expresi\u00f3n estuviera controlada por el promotor inducible de la misina(pmisa).El proceso de autoregulaci\u00f3n de la bios\u00ednteeis de nisina se ha aplicado con \u00e9xito par la producci\u00f3n heter\u00f3loga de p\u00e9ptidos en diversas especies y g\u00e9neros de bacteras l\u00e1cticas.Para ello, se requieren tres elementos esenciales:(a) una cepa de contenga los genes nisrk;(b) la adici\u00f3n de nisina como mol\u00e9cula inductora; y c que el gen de inter\u00e9s est\u00e9 controlado por el promotor pmisa.Las cepas empleadas como hospedadoras fueron l. Lactis fi5876, una capa productora de nisina, y l.Lactis  fi7847 , una cepa no bacteriocinog\u00e9nica.Las dos cepas contienen el transpos\u00f3n tn5301,que porta la agrupaci\u00f3nde genes de la nisina , incluidos los genes nisrk.En el caso de l. Lactis fi7847, el gen estructural de la nisina (nisa) ha sufrido una delecci\u00f3n que impide su expresi\u00f3n y, en consecuencia, la producci\u00f3n de esta bacteriocina.  la introducci\u00f3n de dos pl\u00e1stidos compatibles (un derivado de ptg262 con el<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Producci\u00f3n inducible de lactococina a, pediocina pa-1, colicina v e interleuquina-2 en cepas de lactococcus lactis productoras de nisina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Producci\u00f3n inducible de lactococina a, pediocina pa-1, colicina v e interleuquina-2 en cepas de lactococcus lactis productoras de nisina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Antonio Fern\u00e1ndez Dumont <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 09\/07\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Rodriguez G\u00f3mez Juan  Miguel<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Antonio Ordo\u00f1ez pereda <\/li>\n<li>carmen Torres manrique (vocal)<\/li>\n<li>arjan Narbad (vocal)<\/li>\n<li>eva Rodr\u00edguez m\u00ednguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Antonio Fern\u00e1ndez Dumont La posibilidad de intercambiar tanto los l\u00edderes como los sistemas de secreci\u00f3n y procesado [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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