{"id":69476,"date":"2018-03-09T23:13:58","date_gmt":"2018-03-09T23:13:58","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-funcional-y-de-expresion-de-la-enterocina-as-48-en-enterococcus-faecalis-y-transferencia-a-otras-bacterias-del-acido-lactico\/"},"modified":"2018-03-09T23:13:58","modified_gmt":"2018-03-09T23:13:58","slug":"analisis-funcional-y-de-expresion-de-la-enterocina-as-48-en-enterococcus-faecalis-y-transferencia-a-otras-bacterias-del-acido-lactico","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-funcional-y-de-expresion-de-la-enterocina-as-48-en-enterococcus-faecalis-y-transferencia-a-otras-bacterias-del-acido-lactico\/","title":{"rendered":"An\u00e1lisis funcional y de expresi\u00f3n de la enterocina as-48 en enterococcus faecalis y transferencia a otras bacterias del \u00e1cido l\u00e1ctico"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Matilde Fern\u00e1ndez Rodr\u00edguez <\/strong><\/h2>\n<p>La enterocina as-48 es un p\u00e9ptido circular producido por enterococcus faecalis, que muestra un amplio espectro de acci\u00f3n frente a numerosas bacterias pat\u00f3genas transmitidas por alimentos y alterantes de los mismos. En esta memoria se ha completado la caracterizaci\u00f3n de la regi\u00f3n gen\u00e9tica responsable del car\u00e1cter as-48 clonada en pam401, que consta de los genes as-48abcc1dd1efgh, en la que as-48a es el gen estructural. La caracterizaci\u00f3n de los productos codificados por los cuatro \u00faltimos genes, obtenidamediante an\u00e1lisis fenot\u00edpico de los mutantes de inserci\u00f3n con el transposo\u00f3n tn5 y la aplicaci\u00f3n de programas inform\u00e1ticos de alta fiabilidad, ha permitido conocer que los genes as-48efgh codifican un transportador de tipo-abc implicado en al autoprotecci\u00f3n frente a as-48. En este sistema de transporte se ha identificado la funci\u00f3n de as-48g, en la que se encuentran los dominios de uni\u00f3n a atp y la de las prote\u00ednas as-48eh que presentan cuatro dominios de expansi\u00f3n en membrana, mientras la prote\u00edna as-48f podr\u00eda aumentar la eficacia del transportador. el an\u00e1lisis de transcripci\u00f3n de la regi\u00f3n as-48 realizado mediante northern blot, junto a los resultados derivados de los estudios de complementaci\u00f3n en trans de los genes as-48abc en pam36e llevados a cabo, ha permitido concluir que la regi\u00f3n as-48 se encuentra estructurada en dos operones de expresi\u00f3n constitutiva (as-48abc y as-48c1dd1efgh) aunque presentan promotores internos que permiten la expresi\u00f3n regulada de los genes as-48bc y as-48efgh. la regi\u00f3n as-48 ha sido clonada en vectores propios bacterias del \u00e1cidol\u00e1ctico (pll253-81 y pem76-81), pero ninguna de las nuevas construcciones obtenidas ha igualado la eficacia de pam401-81 en la expresi\u00f3n y resistencia frente a as-48. La transferencia de la regi\u00f3n as-48 a cepas de inter\u00e9s biotecnol\u00f3gico, ha sido realizada con \u00e9xito en lactococcus, lactobacillus y e.Faecium. Las dos primeras s\u00f3lo mostraron una d\u00e9bil expresi\u00f3n de la re<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis funcional y de expresi\u00f3n de la enterocina as-48 en enterococcus faecalis y transferencia a otras bacterias del \u00e1cido l\u00e1ctico<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis funcional y de expresi\u00f3n de la enterocina as-48 en enterococcus faecalis y transferencia a otras bacterias del \u00e1cido l\u00e1ctico <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Matilde Fern\u00e1ndez Rodr\u00edguez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Granada<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/07\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mercedes Maqueda Abreu<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco Gamarro conde <\/li>\n<li> Mart\u00edn mart\u00edn m. cruz (vocal)<\/li>\n<li>fernanda Ru\u00edz larrea (vocal)<\/li>\n<li> Fern\u00e1ndez escamilla Ana m. (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Matilde Fern\u00e1ndez Rodr\u00edguez La enterocina as-48 es un p\u00e9ptido circular producido por enterococcus faecalis, que muestra un [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[17564,152113,3371,152112,152111,35872],"class_list":["post-69476","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-fernanda-ruiz-larrea","tag-fernandez-escamilla-ana-m","tag-francisco-gamarro-conde","tag-martin-martin-m-cruz","tag-matilde-fernandez-rodriguez","tag-mercedes-maqueda-abreu"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/69476","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=69476"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/69476\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=69476"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=69476"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=69476"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}