{"id":69601,"date":"2018-03-09T23:14:06","date_gmt":"2018-03-09T23:14:06","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/mecanismo-de-regulacion-transcripcional-mediana-por-copg-en-el-promotor-pcr-del-plasmido-pmv158\/"},"modified":"2018-03-09T23:14:06","modified_gmt":"2018-03-09T23:14:06","slug":"mecanismo-de-regulacion-transcripcional-mediana-por-copg-en-el-promotor-pcr-del-plasmido-pmv158","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/mecanismo-de-regulacion-transcripcional-mediana-por-copg-en-el-promotor-pcr-del-plasmido-pmv158\/","title":{"rendered":"Mecanismo de regulaci\u00f3n transcripcional mediana por copg en el promotor pcr del pl\u00e1smido pmv158"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ana M. Hernandez Arriaga <\/strong><\/h2>\n<p>Este trabajo experimental se ha centrado en la caracterizaci\u00f3n de del mecanismo de regulaci\u00f3n transcripcional mediada por el represor copg en el promotor pcr del pl\u00e1smido pmv158.El trabajo de investigaci\u00f3n ha incluido el estudio de: 1)los complejos dna-prote\u00edna formados entre la rna polimerasa de escherichia coli y\/o copg,y el promotor pcr, mediante ensayos de retraso en gel y ensayos de protecci\u00f3n y 2)caracterizaci\u00f3n del dna operador de copg mediante la construci\u00f3n de operadores mutantes.Todos los operadores han sido analizados mediante ensayos de protecci\u00f3n frente a radical hidroxilo y metilaci\u00f3n con dms y 3)caracterizaci\u00f3n de la uni\u00f3n de copg a su dna operador mediante la estimaci\u00f3n de la constante de afinidad y la constante de disociaci\u00f3n relativa.  los resultados obtenidos nos han permitido demostrar que la prote\u00edna copg regula la actividad del promotor pcr en dos puntos diferentes del proceso de iniciaci\u00f3n de la transcripci\u00f3n:i)inhibe la uni\u00f3n de la rna polimerasa, y ii)la expulsa de su sitio de uni\u00f3n incluso cuando la polimersa est\u00e1 asentada en el promotor.Adem\u00e1s se ha caracterizado el papel de las regiones que constituyen el operador de copg, y su influencia en la uni\u00f3n de la prote\u00edna al dna.La prote\u00edna se une con alta afinidad y cooperatividad a su dna diana, caracter\u00edsticas de uni\u00f3n esenciales para poder realizar su funci\u00f3n de represor transcripcional.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Mecanismo de regulaci\u00f3n transcripcional mediana por copg en el promotor pcr del pl\u00e1smido pmv158<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Mecanismo de regulaci\u00f3n transcripcional mediana por copg en el promotor pcr del pl\u00e1smido pmv158 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ana M. Hernandez Arriaga <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/07\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gloria Del Solar  Dongil<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Torno garrido <\/li>\n<li>miquel Coll capella (vocal)<\/li>\n<li>ram\u00f3n D\u00edaz orejas (vocal)<\/li>\n<li>ramon Eritja casadell\u00ed\u00a0 (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ana M. Hernandez Arriaga Este trabajo experimental se ha centrado en la caracterizaci\u00f3n de del mecanismo de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[152321,67564,58467,26322,6062,30396],"class_list":["post-69601","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-ana-m-hernandez-arriaga","tag-antonio-torno-garrido","tag-gloria-del-solar-dongil","tag-miquel-coll-capella","tag-ramon-diaz-orejas","tag-ramon-eritja-casadelli"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/69601","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=69601"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/69601\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=69601"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=69601"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=69601"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}