{"id":70065,"date":"2018-03-09T23:14:33","date_gmt":"2018-03-09T23:14:33","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/clonacion-y-estudios-de-expresion-del-factor-de-crecimiento-vasculo-endotelial-vegf-en-trucha-arco-iris-oncorhynchus-mykiss\/"},"modified":"2018-03-09T23:14:33","modified_gmt":"2018-03-09T23:14:33","slug":"clonacion-y-estudios-de-expresion-del-factor-de-crecimiento-vasculo-endotelial-vegf-en-trucha-arco-iris-oncorhynchus-mykiss","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/leon\/clonacion-y-estudios-de-expresion-del-factor-de-crecimiento-vasculo-endotelial-vegf-en-trucha-arco-iris-oncorhynchus-mykiss\/","title":{"rendered":"Clonacion y estudios de expresion del factor de crecimiento vasculo-endotelial (vegf) en trucha arco iris (oncorhynchus mykiss)"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jorge Ria\u00f1o Iglesias <\/strong><\/h2>\n<p>La mayor parte de los avances en el estudio de la vasculog\u00e9nesis y la angiog\u00e9nesis se han realizado en especies de mam\u00edferos, dejando un peque\u00f1o papel para otras especies de vertebrados. As\u00ed, el \u00fanico modelo de angiog\u00e9nesis in vitro desarrollado en un tele\u00f3steo, la trucha arco iris (oncorhynchus mykiss), ha permitido avanzar en le estudio de la vasculog\u00e9nesis en vertebrados inferiores.  en el presente trabajo se ha ampliado el abanico de herramientas disponibles para el estuido de estos dos importantes procesos, realizando la clonaci\u00f3n del principal regulador de la angiog\u00e9nesis, el factor de crecimiento vasculo-endotelial (vegf) de trucha arco iris. Asimismo, se ha realizado la clonaci\u00f3n parcial de un fragmento del gen vegfr-2, que codifica para el receptor principal del vegf, y del factor derivado de plaquetas (pdgf-a). En el caso del vegf, se han clonado dos formas moleculares, una de 122 amino\u00e1cidos y otra de 166 amino\u00e1cidos, producidas pro procesamiento alternativo del arn mensajero. la prote\u00edna vegf122 fue expresada en escherichia coli y la prote\u00edna recombinante purificada a partir de los cuerpos de inclusi\u00f3n producidos.  para estudiar la actividad biol\u00f3gica de la prote\u00edna recombinante se realizaron estudios de expresi\u00f3n g\u00e9nica, mediante la t\u00e9cnica de transcripci\u00f3n reversa y reacci\u00f3n en cadena de la polimerasa (rt-pcr), en las l\u00edneas celulares angiog\u00e9nicas tss y tps-2 de o.Mykiss estimuladas con vegf122 recombinante. los genes estudiados incluyeron: interleukina-1b (il-1b), ciclo-oxigenasa 2(cox2), cadena b del mhc-ii (mhc-iib), y los del propio gen vegf y de su receptor vegfr-2. Como controles positivos de la expresi\u00f3n de estos genes en las l\u00edneas celulares, estos ensayos se realizaron tambi\u00e9n en presencia de los agentes estimulantes lipopolisac\u00e1rido bacteriano (lps) y el factor de la necrosis tumoral (tnf-a) recombinante de o. Mykiss.  estos estudios han permitido concluir que las l\u00edneas celulares ts<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Clonacion y estudios de expresion del factor de crecimiento vasculo-endotelial (vegf) en trucha arco iris (oncorhynchus mykiss)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Clonacion y estudios de expresion del factor de crecimiento vasculo-endotelial (vegf) en trucha arco iris (oncorhynchus mykiss) <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jorge Ria\u00f1o Iglesias <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/09\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Alberto Jose Villena Cortes<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: agustin gregorio Zapata gonzalez <\/li>\n<li>Jos\u00e9 Mar\u00eda Castro gonzalez (vocal)<\/li>\n<li>germ\u00e1n Naharro carrasco (vocal)<\/li>\n<li>Antonio Fernandez medarde (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jorge Ria\u00f1o Iglesias La mayor parte de los avances en el estudio de la vasculog\u00e9nesis y la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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