{"id":71217,"date":"2004-12-11T00:00:00","date_gmt":"2004-12-11T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-expresion-y-regulacion-del-transportador-de-glucosa-glut8-en-tejido-intestinal\/"},"modified":"2004-12-11T00:00:00","modified_gmt":"2004-12-11T00:00:00","slug":"estudio-de-la-expresion-y-regulacion-del-transportador-de-glucosa-glut8-en-tejido-intestinal","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/estudio-de-la-expresion-y-regulacion-del-transportador-de-glucosa-glut8-en-tejido-intestinal\/","title":{"rendered":"Estudio de la expresion y regulacion del transportador de glucosa glut8 en tejido intestinal"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Amparo Romero Pico <\/strong><\/h2>\n<p>Glut8 es un transportador de glucosa recientemente clonado, que se expresa fundamentalmente en test\u00edculo, aunque tambi\u00e9n se ha detectado en otros tejidos como cerebro e intestino. En este trabajo hemos analizado la expresi\u00f3n y localizaci\u00f3n de glut8 en intestino de rat\u00f3n en diferentes situaciones, utilizando t\u00e9cnicas de rt-pcr, western blot e inmunohistoqu\u00edmica, y hemos estudiado la regulaci\u00f3n de su expresi\u00f3n y su funci\u00f3n utilizando m\u00e9todos de transfecci\u00f3n y cultivo celular. Nuestros resultados indican que glut8 se expresa en las c\u00e9lulas epiteliales absortivas de la mucosa en todo el tracto intestinal del rat\u00f3n, incluido el colon, en una localizaci\u00f3n fundamentalmente supranuclear. Hemos observado tambi\u00e9n que en animales sujetos a ayuno durante 24 horas la expresi\u00f3n de glut8 disminuye ligeramente en yeyuno e ileon, mientras que la inducci\u00f3n experimental de un estado de diabetes provoca el incremento de la expresi\u00f3n del transportador en esos mismos segmentos intestinales. Asimismo, hemos detectado, aislado e identificado la expresi\u00f3n adicional de tres variantes transcripcionales producidas por un procesado alternativo de corte y empalme del gen glut8 en intestino de rat\u00f3n, a las que hemos denominado sp1, sp2 y sp3. Hemos estudiado  la posible funci\u00f3n de estas tres variantes en la regulaci\u00f3n de la expresi\u00f3n y funci\u00f3n de glut8 transfectando c\u00e9lulas 3t3-l1 con los diferentes transcritos y hemos observado que tan solo la variante sp3, la m\u00e1s corta de las tres, tiene un efecto sobre la funci\u00f3n de glut8, ya que la transfecci\u00f3n de las c\u00e9lulas con esta variante induce un aumento de la expresi\u00f3n de su arnm y un cambio en la localizaci\u00f3n de la prote\u00edna, que se transloca hacia a la membrana plasm\u00e1tica, provocando con ello, un aumento en el transporte de glucosa al interior de las c\u00e9lulas. Finalmente hemos estudiado la expresi\u00f3n de glut8 en la l\u00ednea celular de carcinoma de colon humano caco-2. Hemos observado que, sorp<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la expresion y regulacion del transportador de glucosa glut8 en tejido intestinal<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la expresion y regulacion del transportador de glucosa glut8 en tejido intestinal <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Amparo Romero Pico <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Cardenal herrera-ceu<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/11\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Emilio Mesonero Gutierrez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: miquel Moreto pedragosa <\/li>\n<li>Ana isabel Alcalde herrero (vocal)<\/li>\n<li> Planas rosell\u00f3 joana Mar\u00eda (vocal)<\/li>\n<li>Mar\u00eda del pilar Lostao crespo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Amparo Romero Pico Glut8 es un transportador de glucosa recientemente clonado, que se expresa fundamentalmente en test\u00edculo, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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