{"id":71774,"date":"2004-10-12T00:00:00","date_gmt":"2004-10-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-de-la-secrecion-de-inmunoglobulinas-en-celulas-plasmaticas-humanas\/"},"modified":"2004-10-12T00:00:00","modified_gmt":"2004-10-12T00:00:00","slug":"estudio-de-la-secrecion-de-inmunoglobulinas-en-celulas-plasmaticas-humanas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/estudio-de-la-secrecion-de-inmunoglobulinas-en-celulas-plasmaticas-humanas\/","title":{"rendered":"Estudio de la secrecion de inmunoglobulinas en celulas plasmaticas humanas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Elena Reales Rodriguez <\/strong><\/h2>\n<p>Las c\u00e9lulas plasm\u00e1ticas juegan un papel cr\u00edtico en la inmunidad adaptativa como mediadores de la respuesta inmune humoral. Estas c\u00e9lulas son linfocitos-b diferenciados con la \u00fanica finalidad de secretar inmunoglobulinas o anticuerpos. A pesar de la importancia de este proceso, no se ha estudiado a\u00fan que mecanismos podr\u00edan estar implicados en la exocitosis de inmunoglobulinas. Por otra parte, se conoce que las proteinas snares (soluble n-ethylmaleimide-sensitive factor attachment protein receptors) median los procesos de fusi\u00f3n de membranas en la c\u00e9lula y se han asociado a procesos de exocitosis en diversos sistemas, por lo que nos planteamos en este trabajo si estas proteinas estar\u00edan involucradas en la exocitosis de anticuerpos.   para el estudio usamos c\u00e9lulas plasm\u00e1ticas humanas normales obtenidas de l\u00e1mina propia de colon y sangre perif\u00e9rica; y tumorales, en este caso las lineas celulares im-9 y u266. Mediante ensayos de detecci\u00f3n de transcritos primarios (rt-pcr y protecci\u00f3n de arnasas) y proteinas (\u00abwestern-blotting\u00bb, inmunofluorescencia y subfraccionamientos celulares) identificamos en las c\u00e9lulas a estudio las proteinas snares, vamp-2, vamp-3, vamp-4, vamp-5, vamp-8, snap-23, sintaxina-2, sintaxina-3, sintaxina-4, proteinas que se han descrito preferentemente en procesos de exocitosis en otros sistemas. Las proteinas vamp-1, snap-25 y sintaxina-1 no se detectan., Las cuales son m\u00e1s espec\u00edficas de tejidos neuronales y neuroendocrinos.   cuando transfectamos las c\u00e9lulas plasm\u00e1ticas con proteinas de fusi\u00f3n gst-snap-23(1-203) observamos una inhibici\u00f3n de la secreci\u00f3n de inmunoglobulinas. Sin embargo, con la transfecci\u00f3n de gst o la forma delecionada gst-snap-23(1-150) (delecci\u00f3n de uni\u00f3n a otros snares) no se veia afectada la secreci\u00f3n. Estos datos sugieren que la prote\u00edna snap-23 parece estar implicada en la secreci\u00f3n de inmunoglobulinas, y las proteinas de fusi\u00f3n para snap-23 estar\u00edan uni\u00e9ndose a prote\u00ednas snares dianas end\u00f3genas, dando lugar a interacciones no productivas, por lo que estar\u00edan actuando como p\u00e9ptidos inhibitorios y bloqueando as\u00ed la exocitosis de anticuerpos.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la secrecion de inmunoglobulinas en celulas plasmaticas humanas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la secrecion de inmunoglobulinas en celulas plasmaticas humanas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Elena Reales Rodriguez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 C\u00e1diz<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 10\/12\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Antonio Campos  Caro<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: carmen Estrada cerquera <\/li>\n<li>Manuel Aguilar diosdado (vocal)<\/li>\n<li>Luis Miguel Guti\u00e9rrez p\u00e9rez (vocal)<\/li>\n<li>v\u00edctor S\u00e1nchez margalet (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Elena Reales Rodriguez Las c\u00e9lulas plasm\u00e1ticas juegan un papel cr\u00edtico en la inmunidad adaptativa como mediadores de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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