{"id":71804,"date":"2018-03-09T23:16:29","date_gmt":"2018-03-09T23:16:29","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-funcional-y-aplicaciones-biotecnologicas-del-promotor-del-gen-end1-de-guisante-pisum-sativum-l\/"},"modified":"2018-03-09T23:16:29","modified_gmt":"2018-03-09T23:16:29","slug":"analisis-funcional-y-aplicaciones-biotecnologicas-del-promotor-del-gen-end1-de-guisante-pisum-sativum-l","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/analisis-funcional-y-aplicaciones-biotecnologicas-del-promotor-del-gen-end1-de-guisante-pisum-sativum-l\/","title":{"rendered":"An\u00e1lisis funcional y aplicaciones biotecnol\u00f3gicas del promotor del gen end1 de guisante (pisum sativum l.)"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Roque Mesa Edelin Marta <\/strong><\/h2>\n<p>End1 es unn gen de guisante (psum sativum l.) Espec\u00edfico de antera que comienza a expresarse desde estadios tempranos del desarrollo (primordio de antera)  en aquellas lineas celulares que dar\u00e1n lugar a los  tejidos epidermis, conectivo, capa intermedia y endotecio, y en estos tejidos una vez desarrollados. La regi\u00f3n promotora de dicho gen es capaz de dirigir la expresi\u00f3n espec\u00edfica del gen uida (gus) a las anteras de plantas de diferentes espeices (arabidopsis thaliana, nicotiana tabacum y lycopersicon esculentum).El patr\u00f3n de expresi\u00f3n del gen gus en esas plantas es similar al del gen end1 en guisante. Esta especificidad que confiere el promotor end1 para la expresi\u00f3n de un gen for\u00e1neo en las anteras ofrec\u00eda la posibilidad de utilizarlo, fusion\u00e1ndolo a un gen citot\u00f3xico, como una herramienta biotecnol\u00f3gica para la obtenci\u00f3n de plantas transg\u00e9nica androest\u00e9riles.  en este trabajo, hemos fusionado la regi\u00f3n 5\u00c2\u00bfcompleta (-2736\/6) del promotor de end1 a la secuencia codificante de la ribonucleasa extracelular producida por el bacillus amyloliquefaciens, barnasa. Con esta construcci\u00f3n hemos transformado dos plantas, modelo, arabidopsis y tabaco y una planta de inter\u00e9s agron\u00f3mico, el tomate. La expresi\u00f3n del gen citot\u00f3xico barnasa donde end1 es activo, trajo como consecuencia una degeneraci\u00f3n de tejidos de la antera que inhibi\u00f3 el desarrollo de los granos de polen. Las plantas transg\u00e9nicas resultantes fueron adroest\u00e9riles. Los estudios a nivel histol\u00f3gico de las anteras transg\u00e9nicas mostraron que los efectos de la barnasa comienzan a observarse muy pronto en el primordio de antera, cuando este est\u00e1 consttuido solo opor c\u00e9lulas indiferenciadas, y contin\u00faa observ\u00e1ndose a lo largo del desarrollo de la misma. De manera general, al final del programa de desarrollo, las anteras transg\u00e9nicas eran de menor tama\u00f1o, su morfolog\u00eda era distinta de las de las anteras silvestres correspondientes, y en su interior se obser<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>An\u00e1lisis funcional y aplicaciones biotecnol\u00f3gicas del promotor del gen end1 de guisante (pisum sativum l.)<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 An\u00e1lisis funcional y aplicaciones biotecnol\u00f3gicas del promotor del gen end1 de guisante (pisum sativum l.) <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Roque Mesa Edelin Marta <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de Valencia<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/12\/2004<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 P\u00edo Beltr\u00e1n Porter<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: victoriano Valpuesta fernandez <\/li>\n<li>Rafael Lozano ruiz (vocal)<\/li>\n<li>Luis Navarro lucas (vocal)<\/li>\n<li>julio Salinas mu\u00f1oz (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Roque Mesa Edelin Marta End1 es unn gen de guisante (psum sativum l.) Espec\u00edfico de antera que [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[509,849,972,494,35,534,16820],"tags":[9902,8069,12193,9228,156347,2344],"class_list":["post-71804","post","type-post","status-publish","format-standard","hentry","category-agronomia","category-biologia-celular","category-botanica","category-ciencias-agrarias","category-ciencias-de-la-vida","category-genetica-vegetal","category-politecnica-de-valencia","tag-jose-pio-beltran-porter","tag-julio-salinas-munoz","tag-luis-navarro-lucas","tag-rafael-lozano-ruiz","tag-roque-mesa-edelin-marta","tag-victoriano-valpuesta-fernandez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/71804","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=71804"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/71804\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=71804"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=71804"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=71804"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}