{"id":7329,"date":"1995-01-01T00:00:00","date_gmt":"1995-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/1995\/01\/01\/estudio-de-la-exocitosis-mediante-deteccion-electroquimica-y-tecnica-de-patch-clamp-en-celulas-cebadas\/"},"modified":"1995-01-01T00:00:00","modified_gmt":"1995-01-01T00:00:00","slug":"estudio-de-la-exocitosis-mediante-deteccion-electroquimica-y-tecnica-de-patch-clamp-en-celulas-cebadas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/estudio-de-la-exocitosis-mediante-deteccion-electroquimica-y-tecnica-de-patch-clamp-en-celulas-cebadas\/","title":{"rendered":"Estudio de la exocitosis mediante deteccion electroquimica y tecnica de \u00abpatch-clamp\u00bb en celulas cebadas"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Rafael Fern\u00e1ndez Chac\u00f3n <\/strong><\/h2>\n<p>La exocitosis es un mecanismo que las celulas utilizan para liberar al medio extracelular macromoleculas no permeables a traves de la membrana plasmatica. Para estudiar las ultimas etapas de este proceso hemos desarrollado un nuevo abordaje electrofisiologico que utiliza la tecnica de \u00abpatch-clamp\u00bb para monitorizar la fusion de vesiculas secretoras individuales en combinacion con la deteccion electroquimica de productos de secrecion utilizando microelectrodos de fibra de carbono en celulas cebadas aisladas. Se han estudiado los cambios de superficie de la membrana celular durante la exocitosis simultaneamente con la deteccion de los productos secretados, determinando que la sustancia detectada es la serotonina, y definiendo las caracteristicas de la liberacion cuantica de serotonina desde una sola vesicula de secrecion. Estudiando el flujo de sustancias a traves del poro de fusion, un canal acuoso que conecta el interior de la vesicula con el medio extracelular durante la exocitosis, hemos observado que la liberacion del contenido vesicular esta controlada por la apertura y cierre de este poro, y se produce liberacion del contenido durante las fusiones reversibles de las vesiculas de secrecion con la membrana celular. El material almacenado dentro de la vesicula lo constituye un contingente mayoritario de moleculas atrapadas en una matriz de proteoglicano osmoticamente inactivo, y un contingente minoritario de moleculas en libre disolucion.  la matriz de proteoglicano dosifica la cantidad liberada durante las fusiones reversibles. Este es un nuevo mecanismo de exocitosis que impide la mezcla de las proteinas de la membrana vesicular y la membrana celular, es mas economico para la celula y explica aspectos de la transmision sinaptica que han sido objeto de debate durante las dos ultimas decadas. Tambien hemos observado que la expansion del poro de fusion se modula por los niveles de calcio intracelular, lo que supone un nuevo punt<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio de la exocitosis mediante deteccion electroquimica y tecnica de \u00abpatch-clamp\u00bb en celulas cebadas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio de la exocitosis mediante deteccion electroquimica y tecnica de \u00abpatch-clamp\u00bb en celulas cebadas <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Rafael Fern\u00e1ndez Chac\u00f3n <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Sevilla<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1995<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Guillermo Alvarez De Toledo Naranjo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 L\u00f3pez Barneo <\/li>\n<li>Ana Ilunda\u00edn Larra\u00f1eta (vocal)<\/li>\n<li>Jordi Marsal Teb\u00e9 (vocal)<\/li>\n<li>Francisco Barros De La Roza (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Rafael Fern\u00e1ndez Chac\u00f3n La exocitosis es un mecanismo que las celulas utilizan para liberar al medio extracelular [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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