{"id":73521,"date":"2018-03-09T23:18:26","date_gmt":"2018-03-09T23:18:26","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/regulacion-de-la-expresion-del-genoma-mitocondrial-efectos-tejido-especa%c2%adficos-de-las-hormonas-tiroideas\/"},"modified":"2018-03-09T23:18:26","modified_gmt":"2018-03-09T23:18:26","slug":"regulacion-de-la-expresion-del-genoma-mitocondrial-efectos-tejido-especa%c2%adficos-de-las-hormonas-tiroideas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/regulacion-de-la-expresion-del-genoma-mitocondrial-efectos-tejido-especa%c2%adficos-de-las-hormonas-tiroideas\/","title":{"rendered":"Regulaci\u00f3n de la expresi\u00f3n del genoma mitocondrial.  efectos tejido-espec\u00edficos de las hormonas tiroideas."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Erika Mar\u00eda Fern\u00e1ndez-vizarra Bailey <\/strong><\/h2>\n<p>El trabajo se ha dividido en tres l\u00edneas: 1.- Calidad de las preparaciones mitocondriales los ensayos in organello han constituido una parte importante en la metodolog\u00eda empleada para el desarrollo del trabajo en la tesis.  Para llevar a cabo este tipo de ensayos es necesaria la obtenci\u00f3n de muestras de mitocondrias aisladas que se realiza b\u00e1sicamente mediante tres pasos: 1) ruptura celular; 2) centrifugaci\u00f3n diferencial y 3) lavados de la fracci\u00f3n cruda mitocondrial. Al evaluar el rendimiento, pureza y funcionalidad de las preparaciones mitocondriales obtenidas seg\u00fan el m\u00e9todo empleado, se ha comprobado que son perfectamente funcionales y aceptablemente puras y totalmente adecuadas para los ensayos de transcripci\u00f3n y s\u00edntesis de prote\u00ednas in organello. Adem\u00e1s, se ha comprobado que el \u00faltimo paso de lavado elimina eficientemente los principales contaminantes sin p\u00e9rdida de mitocondrias. 2.- Diferencias en la capacidad oxphos entre distintos \u00f3rganos se valoraron las diferencias existentes entre distintos \u00f3rganos en cuanto a la actividad de fosforilaci\u00f3n oxidativa (oxphos), con el fin de determinar cual es el peso de la regulaci\u00f3n de la expresi\u00f3n del dna mitocondrial (mtdna) en cada uno de los diferentes tejidos y analizar cuales son las etapas m\u00e1s relevantes y los factores implicados en dicha regulaci\u00f3n. Para establecer comparaciones se eligieron cinco tejidos: cerebro, coraz\u00f3n, h\u00edgado, m\u00fasculo esquel\u00e9tico y ri\u00f1\u00f3n. Se tomaron muestras de ratas hembra y macho para determinar actividad de citocromo c oxidasa (cox) y de citrato sintasa (cs), adem\u00e1s de niveles de mtdna con respecto al dna nuclear (ndna). A partir de este estudio se propone la utilizaci\u00f3n de la normalizaci\u00f3n de los par\u00e1metros mitocondriales por cantidad de mtdna y ndna para facilitar la comparaci\u00f3n entre tipos celulares y situaciones fisiol\u00f3gicas diferentes. Se ha confirmado la existencia de diferencias en la actividad respiratoria mitocondrial entre los \u00f3rganos anali<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Regulaci\u00f3n de la expresi\u00f3n del genoma mitocondrial.  efectos tejido-espec\u00edficos de las hormonas tiroideas.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Regulaci\u00f3n de la expresi\u00f3n del genoma mitocondrial.  efectos tejido-espec\u00edficos de las hormonas tiroideas. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Erika Mar\u00eda Fern\u00e1ndez-vizarra Bailey <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Zaragoza<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 13\/04\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Patricio Fern\u00e1nez Silva<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 manuel Cuezva marcos <\/li>\n<li>francesc Villarroya gombau (vocal)<\/li>\n<li>victor Sorribas alejaldre (vocal)<\/li>\n<li>Ana Mar\u00eda P\u00e9rez castillo (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Erika Mar\u00eda Fern\u00e1ndez-vizarra Bailey El trabajo se ha dividido en tres l\u00edneas: 1.- Calidad de las preparaciones [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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