{"id":73712,"date":"2018-03-09T23:18:38","date_gmt":"2018-03-09T23:18:38","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/diferenciacion-de-celulas-madre-embrionarias-de-raton-hacia-celulas-productoras-de-insulina\/"},"modified":"2018-03-09T23:18:38","modified_gmt":"2018-03-09T23:18:38","slug":"diferenciacion-de-celulas-madre-embrionarias-de-raton-hacia-celulas-productoras-de-insulina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/diferenciacion-de-celulas-madre-embrionarias-de-raton-hacia-celulas-productoras-de-insulina\/","title":{"rendered":"Diferenciacion de celulas madre embrionarias de raton hacia celulas productoras de insulina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jonathan Richard Jones Barbera <\/strong><\/h2>\n<p>La diabetes es una de las enfermedades m\u00e1s comunes del mundo, con una preValencia del 2-5% y en continuo crecimiento. El tratamiento de dicha enfermedad, actualmente incurable, consiste principalmente en dieta equilibrada, ejercicio fisico e inyecciones de insulina. Sin embargo, en los ultimos 5 a\u00f1os se ha conseguido trasplantar islotes pancre\u00e1ticos en pacientes diab\u00e9ticos con una tasa de \u00e9xito muy alta. Desafortunadamente, existe una gran falta de donantes, pudiendo cubrir unicamente el 1% de los casos de diab\u00e9ticos. por es necesario buscar fuentes alternativas para obtener c\u00e9lulas beta pancre\u00e1ticas, como puede ser la diferenciaci\u00f3n de c\u00e9lulas madre embrionarias hacia c\u00e9lulas productoras de insulina.  en este trabajo se dise\u00f1a un protocolo de diferenciaci\u00f3n de c\u00e9lulas madre embrionarias de rat\u00f3n hacia c\u00e9lulas productoras de insulina, empleando factores ex\u00f3genos que dirijan la diferenciaci\u00f3n de las c\u00e9lulas hacia un destino pancre\u00e1tico. A partir de estas c\u00e9lulas diferenciadas se seleccionan las c\u00e9lulas productoras de insulina mediante un plasmido que contiene el promotor del gen nkx6.1, un factor de transcripci\u00f3n espec\u00edfico de la c\u00e9lula beta. Las c\u00e9lulas resultantes se analizar\u00e1n a nivel g\u00e9nico mediante rt-pcr, a nivel proteico mediante inmunocitoqu\u00edmica e radioinmunoan\u00e1lisis, y funcional mediante estudios de secreci\u00f3n de insulina y trasplante en ratones diab\u00e9ticos.  como resultado se obtuvieron c\u00e9lulas que produc\u00edan insulina y respondieron frente a concentraciones estimulantes de glucosa.  Al trasplantar en ratones diab\u00e9ticos se consegui\u00f3 normalizar sus glucemias durante todo el tiempo del experimento. Experimentos de inmunohistoquimica y el efecto en la glucemia tras retirar el trasplante mostraron que las c\u00e9lulas trasplantadas fueron las responsables de la normalizaci\u00f3n de la glucemia y no una posible regeneraci\u00f3n de las c\u00e9lulas beta del rat\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Diferenciacion de celulas madre embrionarias de raton hacia celulas productoras de insulina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Diferenciacion de celulas madre embrionarias de raton hacia celulas productoras de insulina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jonathan Richard Jones Barbera <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Miguel hern\u00e1ndez de elche<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/04\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Bernat Soria Escoms<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francisco xavier Real arribas <\/li>\n<li>Jorge Ferrer marrades (vocal)<\/li>\n<li>Francisco Javier Bedoya bergua (vocal)<\/li>\n<li>Miguel Torres sanchez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jonathan Richard Jones Barbera La diabetes es una de las enfermedades m\u00e1s comunes del mundo, con una [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[849,35,9399,2135,817,37169],"tags":[11639,67061,13679,159951,159952,38636],"class_list":["post-73712","post","type-post","status-publish","format-standard","hentry","category-biologia-celular","category-ciencias-de-la-vida","category-cultivo-celular","category-fisiologia-endocrina","category-fisiologia-humana","category-miguel-hernandez-de-elche","tag-bernat-soria-escoms","tag-francisco-javier-bedoya-bergua","tag-francisco-xavier-real-arribas","tag-jonathan-richard-jones-barbera","tag-jorge-ferrer-marrades","tag-miguel-torres-sanchez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/73712","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=73712"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/73712\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=73712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=73712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=73712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}