{"id":73953,"date":"2005-12-05T00:00:00","date_gmt":"2005-12-05T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/metodologa%c2%ada-dbd-fish-estudio-de-la-estructura-cromatinica-y-del-dano-radioinducido\/"},"modified":"2005-12-05T00:00:00","modified_gmt":"2005-12-05T00:00:00","slug":"metodologa%c2%ada-dbd-fish-estudio-de-la-estructura-cromatinica-y-del-dano-radioinducido","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/metodologa%c2%ada-dbd-fish-estudio-de-la-estructura-cromatinica-y-del-dano-radioinducido\/","title":{"rendered":"Metodolog\u00eda dbd-fish: estudio de la estructura cromat\u00ednica y del da\u00f1o radioinducido"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Rivero Gil Mar\u00eda Teresa <\/strong><\/h2>\n<p>Mediante el empleo de la t\u00e9cnica dbd-fish se ha analizado la se\u00f1al basal en las c\u00e9lulas de rat\u00f3n, encontr\u00e1ndose que el adn sat\u00e9lite principal es el responsable mayoritario de dicha se\u00f1al basal, pudiendo ser debida a la presencia de abundantes lugares l\u00e1biles alcalinos constitutivos en esta secuencia.  adem\u00e1s, la t\u00e9cnica dbd-fish en cometas neutros permiti\u00f3 la estima simult\u00e1nea y f\u00e1cil de las roturas de cadena sencilla (ssb) y doble (dsb) en el mismo n\u00facleo, c\u00e9lula a c\u00e9lula. Las ssb se relacionan con la intensidad de la se\u00f1al de dbd-fish y las dsb con la longitud de migraci\u00f3n del cometa en la misma se\u00f1al de dbd-fish.  combinando la t\u00e9cnica de dbd-fish con la electroforesis en gel en c\u00e9lulas individuales (scge), se realiz\u00f3 un estudio de los patrones de migraci\u00f3n del adn en electroforesis de c\u00e9lulas aisladas, inclu\u00eddas en microgel en dos dimensiones. La migraci\u00f3n del adncs tras la segunda electroforesis alcalina (electroforesis neutra-desnaturalizaci\u00f3n alcalina-90\u00c2\u00ba electroforesis alcalina), se va haciendo progresivamente mayor y m\u00e1s homog\u00e9nea, movi\u00e9ndose desde la cabeza a la cola del cometa obtenido en la primera electroforesis. debido a las condiciones desnaturalizantes, la longitud de adncs aumenta con el tiempo y estos tramos de adncs tienden a no interactuar entre ellos, facilit\u00e1ndose su movilidad. En el caso de la segunda electroforesis neutra (electroforesis neutra-desnaturalizaci\u00f3n alcalina-90\u00c2\u00ba electroforesis neutra), la migraci\u00f3n del adncs se va haciendo progresivamente menor y m\u00e1s homog\u00e9nea, movi\u00e9ndose desde la cabeza a la cola del cometa obtenido en la primera electroforesis. En este caso, las condiciones de ph permiten la renaturalizaci\u00f3n parcial del adncs generado durante el paso previo de desnaturalizaci\u00f3n. por lo tanto, bajo estas condiciones, los fragmentos de renaturalizaci\u00f3n parcial del adncs se entrelazar\u00edan, dando lugar a una menor libertad de migraci\u00f3n, que se va acentuando seg<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Metodolog\u00eda dbd-fish: estudio de la estructura cromat\u00ednica y del da\u00f1o radioinducido<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Metodolog\u00eda dbd-fish: estudio de la estructura cromat\u00ednica y del da\u00f1o radioinducido <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Rivero Gil Mar\u00eda Teresa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 A coru\u00f1a<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 12\/05\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Luis Fernandez Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jaime Gos\u00e1lvez berenguer <\/li>\n<li>anna Genesc\u00e1 garrigosa (vocal)<\/li>\n<li>vicente Goyanes villaescusa (vocal)<\/li>\n<li>Francisco Javier Blanco Garc\u00eda (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Rivero Gil Mar\u00eda Teresa Mediante el empleo de la t\u00e9cnica dbd-fish se ha analizado la se\u00f1al basal [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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