{"id":74201,"date":"2018-03-09T23:19:11","date_gmt":"2018-03-09T23:19:11","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/reduccion-de-la-eficiencia-energetica-en-mitocondrias-hepaticas-mediante-transferencia-genica-de-ucpi\/"},"modified":"2018-03-09T23:19:11","modified_gmt":"2018-03-09T23:19:11","slug":"reduccion-de-la-eficiencia-energetica-en-mitocondrias-hepaticas-mediante-transferencia-genica-de-ucpi","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/procesos-metabolicos\/reduccion-de-la-eficiencia-energetica-en-mitocondrias-hepaticas-mediante-transferencia-genica-de-ucpi\/","title":{"rendered":"Reducci\u00f3n de la eficiencia energ\u00e9tica en mitocondrias hep\u00e1ticas mediante transferencia g\u00e9nica de ucpi"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Pedro Gonzalez Muniesa <\/strong><\/h2>\n<p>Titulo: reducci\u00f3n de la eficiencia energ\u00e9tica en mitocondrias hep\u00e1ticas mediante transferencia g\u00e9nica de ucpl resumen: most current obesity treatments are based on increasing energy expenditure diminishing energy intake. In this sense, the stimulation of energy rel\u00e9ase heat, known as adaptive thermogenesis, would influence the whole energy expenditure. The uncoupling protein 1 (ucpl) or thermogenin , an internal mitochondrial membrane protein uniquely expressed in brown adipose tissue, the main molecule responsible for the adaptive thermogenesis. This protein dissipates the prot\u00f3n gradient generated in the mitocondrial respiration producing heat, diminishing mitochondria energy efficiency. Gene transfer would allow expressing ucp1 in other tissues that could be suitable for obesity treatment in humans. The aim of this study was to ectopically express ucpl by gene transfer in \u00abliver mitochondria (\u00abin vitro\u00bb and \u00abin vivo) in order to affect energy utilization. \u00c2\u00bfin vitro\u00bb, the effect of ectopic protein expression on energy metabolism of hepg2 cells showed that cells expressing ucpl had decreased atp production. \u00abIn vivo\u00bb, mitochondria from liver of ectopic ucpl transferred mice showed a decrease on atp production, lasted more time in the membrane potential state 3, and consumed more molecular oxygen to produce the same amount of atp than those of control group. Furthermore, the ucpl transferred group tended to have a smaller respiratory control ratio than the control group. In summary, gene transfer in liver cells to ectopically express the ucpl protein could be an approach to manage metabolic disorders related to energy efficiency and expenditure, such as obesity, by dissipating energy excess.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Reducci\u00f3n de la eficiencia energ\u00e9tica en mitocondrias hep\u00e1ticas mediante transferencia g\u00e9nica de ucpi<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Reducci\u00f3n de la eficiencia energ\u00e9tica en mitocondrias hep\u00e1ticas mediante transferencia g\u00e9nica de ucpi <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Pedro Gonzalez Muniesa <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/05\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Alfredo Martinez Hernandez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: esteban Santiago calvo <\/li>\n<li>Jos\u00e9 atonio Fernandez lopez (vocal)<\/li>\n<li>francesca Serra vich (vocal)<\/li>\n<li>Marta Garaulet aza (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Pedro Gonzalez Muniesa Titulo: reducci\u00f3n de la eficiencia energ\u00e9tica en mitocondrias hep\u00e1ticas mediante transferencia g\u00e9nica de ucpl [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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