{"id":74275,"date":"2005-02-06T00:00:00","date_gmt":"2005-02-06T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-de-las-subunidades-de-union-a-nucleotido-del-transportador-abc-de-nitrato-nitrito-de-la-cianobacteria-phormidium-laminosum\/"},"modified":"2005-02-06T00:00:00","modified_gmt":"2005-02-06T00:00:00","slug":"caracterizacion-de-las-subunidades-de-union-a-nucleotido-del-transportador-abc-de-nitrato-nitrito-de-la-cianobacteria-phormidium-laminosum","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/caracterizacion-de-las-subunidades-de-union-a-nucleotido-del-transportador-abc-de-nitrato-nitrito-de-la-cianobacteria-phormidium-laminosum\/","title":{"rendered":"Caracterizaci\u00f3n de las subunidades de uni\u00f3n a nucle\u00f3tido del transportador abc de nitrato\/nitrito de la cianobacteria phormidium laminosum"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Marta Llarena Fern\u00e1ndez <\/strong><\/h2>\n<p>En cianobacterias el nitrato\/nitrito se transportan al inferior celular mediante un transportador \u00abatp-binding cassette\u00bb (abc) denominado nrt, compuesto por cuatro subunidades proteicas: nrta (subunidad peripl\u00e1smica responsable de la uni\u00f3n del nitrato\/nitrito), nrtb (subunidad transmembranal), nrtc y nrtd (subunidades de uni\u00f3n a nucle\u00f3tido). En la cianobacteria filamentosa, term\u00f3fila y no fijadora de nitr\u00f3geno, phormidium laminosum se hab\u00edan identificado los genes nrta, nrtb y nrtc, que codifican el transportador abc de nitrato\/nitrito, formado parte del oper\u00f3n nir, junto con el gen mira, que codifica la nitrito reductasa.  en este trabajo se ha identificado el gen nrtd, a continuaci\u00f3n de mrtc, formando parte del oper\u00f3n nir. La subunidad nrtc del transportador de nitrato\/nitrito de p.Laminosum se ha purificado a partir de c\u00e9lulas deficientes en nitr\u00f3geno y se ha comprobado que hidroliza atp in vitro, en ausencia del resto de componentes del transportador. Los genes nrtc y nrtd se han clonado en pl\u00e1smidos de expresi\u00f3n pqe-9 y se han expresado en e.Coli, purific\u00e1ndose como prote\u00ednas de fusi\u00f3n a cola de histidinas. Se han caracterizado las subunidades his6nrtc e his6nrtd por su capacidad para catalizar la hidr\u00f3lisis de atp, con respecto a su especificidad por varios sustratos y sensibilidad a inhibidores, demostr\u00e1ndose la que ambas prote\u00ednas hidrolizan atp in vitro. adem\u00e1s, mediante elctroforesis en geles de poliacrilamida en condiciones no desnaturalizantes se ha detectado la formaci\u00f3n de homod\u00edmeros de nrtc, his6nrtc e his6nrtd. La formaci\u00f3n de homod\u00edmeros de his6nrtd tambi\u00e9n se ha comprobado por espectrometr\u00eda de masas maldi-tof.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n de las subunidades de uni\u00f3n a nucle\u00f3tido del transportador abc de nitrato\/nitrito de la cianobacteria phormidium laminosum<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n de las subunidades de uni\u00f3n a nucle\u00f3tido del transportador abc de nitrato\/nitrito de la cianobacteria phormidium laminosum <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Marta Llarena Fern\u00e1ndez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Pa\u00eds vasco\/euskal herriko unibertsitatea<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 02\/06\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Juan  Luis Serra Ferrer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 Mar\u00eda Vega piqueres <\/li>\n<li>Francisco Javier Florencio bellido (vocal)<\/li>\n<li>Emilio Fernandez reyes (vocal)<\/li>\n<li>Mar\u00eda Jos\u00e9 Bonete  p\u00e9rez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Marta Llarena Fern\u00e1ndez En cianobacterias el nitrato\/nitrito se transportan al inferior celular mediante un transportador \u00abatp-binding cassette\u00bb [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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