{"id":75534,"date":"2005-08-07T00:00:00","date_gmt":"2005-08-07T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-de-un-test-de-quimiosensibilidad-para-los-tumores-vesicales-superficiales-basados-en-el-cultivo-tridimensional-de-esferoides-tumorales-procedentes-de-biopsia\/"},"modified":"2005-08-07T00:00:00","modified_gmt":"2005-08-07T00:00:00","slug":"desarrollo-de-un-test-de-quimiosensibilidad-para-los-tumores-vesicales-superficiales-basados-en-el-cultivo-tridimensional-de-esferoides-tumorales-procedentes-de-biopsia","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/desarrollo-de-un-test-de-quimiosensibilidad-para-los-tumores-vesicales-superficiales-basados-en-el-cultivo-tridimensional-de-esferoides-tumorales-procedentes-de-biopsia\/","title":{"rendered":"Desarrollo de un test de quimiosensibilidad para los tumores vesicales superficiales basados en el cultivo tridimensional de esferoides tumorales procedentes de biopsia"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Juan  Pablo Burgu\u00e9s Gasi\u00f3n <\/strong><\/h2>\n<p>Introducci\u00f3n: la eficacia de la quimioterapia intravesical como tratamiento adyuvante tras la rtu en los tumores vesicales superficiales se ve limitada por las resistencias a f\u00e1rmacos individuales o multirresistencias a varios agentes. Se han desarrollado distintos tests de quimiosensibilidad in vitro, pero la mayor\u00eda basados en cultivos en monocapa o suspensi\u00f3n de c\u00e9lulas individuales. El cultivo tridimensional de esferoides tumorales conserva las interacciones celulares y la arquitectura tisular originales, por lo que constituye un modelo m\u00e1s pr\u00f3ximo a la realidad, al ser capaz de reproducir los mecanismos moleculares de las resistencias tanto unicelulares como multicelulares.  objetivos: 1) desarrollar un test de quimiosensibilidad a los agentes intravesicales comunmente utilizados en la pr\u00e1ctica cl\u00ednica: epirubicina (epi), adriamicina (adr), tiotepa (tio) y mitomicina c (mmc). 2) investigar el efecto del verapamilo (vpm) sobre la resistencia a la epirubicina. 3) determinar la citotoxicidad inducida por el ciprofloxacino (cipro) s\u00f3lo o en combinaci\u00f3n con la epirubicina.  material y m\u00e9todos: se tomaron 2 biopsias con pinza fr\u00eda en 40 pacientes. las muestras fueron fragmentadas, introducidas en un frasco con base de agar y sobrenadante de medio de eagle modificado por dulbecco suplementado, y puestas a cultivar en una atm\u00f3sfera de 5% co2, a 37\u00c2\u00bac y con humedad relativa del 100%. A los 2 d\u00edas se seleccionaron 72 esferoides de cada tumor bajo visi\u00f3n microsc\u00f3pica y se transfirieron de 3 en 3 a una placa con 24 pocillos, 6 de ellos con cipro 0,2 mg\/ml. 3 d\u00edas despu\u00e9s se a\u00f1adi\u00f3: 1) pocillos 1-3: epi 1 mg\/ml; 2) pocillos 4-6: epi 1 mg\/ml + vpm 0,5 mg\/ml; 3) pocillos 7-9: adr 1 mg\/ml; 4) pocillos 10-12: thio 1 mg\/ml; 5) pocillos 13-15: mmc 1 mg\/ml; 6) pocillos 16-18: epi 1 mg\/ml + cipro 0,2 mg\/ml; 7) pocillos 19-21: cipro 0,2 mg\/ml; 8) pocillos 22-24: control sin quimioter\u00e1pico. al cabo de 2 horas se procedi\u00f3 a la disgr<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Desarrollo de un test de quimiosensibilidad para los tumores vesicales superficiales basados en el cultivo tridimensional de esferoides tumorales procedentes de biopsia<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo de un test de quimiosensibilidad para los tumores vesicales superficiales basados en el cultivo tridimensional de esferoides tumorales procedentes de biopsia <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Juan  Pablo Burgu\u00e9s Gasi\u00f3n <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 08\/07\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Fernando Jimenez Cruz<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: remigio Vela navarrete <\/li>\n<li>Francisco Javier Vera sempere (vocal)<\/li>\n<li>Jes\u00fas Romero maroto (vocal)<\/li>\n<li>joaqu\u00edn Carballido rodr\u00edguez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Juan Pablo Burgu\u00e9s Gasi\u00f3n Introducci\u00f3n: la eficacia de la quimioterapia intravesical como tratamiento adyuvante tras la rtu [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[9,141,1533],"tags":[84201,163277,24170,14430,163276,37510],"class_list":["post-75534","post","type-post","status-publish","format-standard","hentry","category-ciencias-medicas","category-cirugia","category-cirugia-urologica","tag-fernando-jimenez-cruz","tag-francisco-javier-vera-sempere","tag-jesus-romero-maroto","tag-joaquin-carballido-rodriguez","tag-juan-pablo-burgues-gasion","tag-remigio-vela-navarrete"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/75534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=75534"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/75534\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=75534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=75534"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=75534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}