{"id":75738,"date":"2018-03-09T23:21:02","date_gmt":"2018-03-09T23:21:02","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-y-caracterizacion-de-abg1-un-gen-esencial-del-hongo-patogeno-oportunista-candida-albicans\/"},"modified":"2018-03-09T23:21:02","modified_gmt":"2018-03-09T23:21:02","slug":"aislamiento-y-caracterizacion-de-abg1-un-gen-esencial-del-hongo-patogeno-oportunista-candida-albicans","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/aislamiento-y-caracterizacion-de-abg1-un-gen-esencial-del-hongo-patogeno-oportunista-candida-albicans\/","title":{"rendered":"Aislamiento y caracterizaci\u00f3n de abg1, un gen esencial del hongo pat\u00f3geno oportunista candida albicans."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Ver\u00f3nica Veses Jim\u00e9nez <\/strong><\/h2>\n<p>Mediante el inmunorrastreo de una genoteca de expresi\u00f3n de candida albicans con un anticuerpo policlonal espec\u00edfico de la forma micelial de dicho hongo (pab anti-gt), se ha aislado, clonado y caracterizado un nuevo gen de este microorganismo. Dicho gen est\u00e1 contenido en el contig 5-3205 del proyecto de secuenciaci\u00f3n del genoma de c. Albicans, y contiene un marco abierto de lectura que codifica para un polip\u00e9ptido putativo de 288 amino\u00e1cidos, con un peso molecular aproximado de 32.921 da. La represi\u00f3n del gen aislado ocasion\u00f3 un severo defecto en la morfog\u00e9nesis de c. Albicans, caracterizado por la formaci\u00f3n de cadenas de levaduras, por lo que el gen fue denominado abg1 (del ingl\u00e9s,  \u00abaltered budding growth\u00bb), en base al fenotipo de crecimiento por gemaci\u00f3n alterado observado en las cepas mutantes para dicho gen. Ensayos de inmunotransferencia de fracciones subcelulares con un anticuerpo policlonal frente a abg1p, y estudios de fusi\u00f3n con la prote\u00edna verde fluorescente (gfp) de aequorea victoria, revelaron que abg1p se  localizaba en la membrana vacuolar de c. Albicans. El patr\u00f3n de fluorescencia de la prote\u00edna abg1p-gfp colocaliz\u00f3 con el observado tras tinci\u00f3n con fm4-64, agente que ti\u00f1e la membrana vacuolar y  de  ves\u00edculas endoc\u00edticas. Con la finalidad de establecer el papel de abg1 en la biolog\u00eda de c. Albicans se procedi\u00f3 a la construcci\u00f3n de un mutante nulo, que no pudo llevarse a cabo a pesar de utilizar dos t\u00e9cnicas de interrupci\u00f3n g\u00e9nica distintas. Se procedi\u00f3 a la construcci\u00f3n de un mutante condicional, en el que la primera copia de abg1 estaba interrumpida con el cassette ura-blaster y la segunda copia quedaba situada bajo el control del promotor de met3. Al reprimir la expresi\u00f3n de abg1, por la acci\u00f3n represora de la metionina y ciste\u00edna sobre el promotor de met3, el mutante fue incapaz de crecer, lo que estableci\u00f3 el car\u00e1cter esencial de abg1 para c. Albicans. El an\u00e1lisis fenot\u00edpico del mutant<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento y caracterizaci\u00f3n de abg1, un gen esencial del hongo pat\u00f3geno oportunista candida albicans.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento y caracterizaci\u00f3n de abg1, un gen esencial del hongo pat\u00f3geno oportunista candida albicans. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Ver\u00f3nica Veses Jim\u00e9nez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 14\/07\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Pedro Martinez Garcia<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: rafael Sentandreu ramon <\/li>\n<li>guillermo Quind\u00f3s andr\u00e9s (vocal)<\/li>\n<li>\u00e1ngel Dom\u00ednguez olavarri (vocal)<\/li>\n<li>a.r. Gow neil (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Ver\u00f3nica Veses Jim\u00e9nez Mediante el inmunorrastreo de una genoteca de expresi\u00f3n de candida albicans con un anticuerpo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,984,278],"tags":[72732,9537,13560,1259,9886,163623],"class_list":["post-75738","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-levaduras","category-microbiologia","tag-a-r-gow-neil","tag-angel-dominguez-olavarri","tag-guillermo-quindos-andres","tag-jose-pedro-Martinez-garcia","tag-rafael-sentandreu-ramon","tag-veronica-veses-jimenez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/75738","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=75738"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/75738\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=75738"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=75738"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=75738"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}