{"id":76240,"date":"2018-03-09T23:21:35","date_gmt":"2018-03-09T23:21:35","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-y-caracterizacion-de-celulas-madre-adultas-pluripotenciales-de-medula-osea-humana-y-de-mono-cynomologus\/"},"modified":"2018-03-09T23:21:35","modified_gmt":"2018-03-09T23:21:35","slug":"aislamiento-y-caracterizacion-de-celulas-madre-adultas-pluripotenciales-de-medula-osea-humana-y-de-mono-cynomologus","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/cultivo-celular\/aislamiento-y-caracterizacion-de-celulas-madre-adultas-pluripotenciales-de-medula-osea-humana-y-de-mono-cynomologus\/","title":{"rendered":"Aislamiento y caracterizaci\u00f3n de c\u00e9lulas madre adultas pluripotenciales de m\u00e9dula \u00f3sea humana y de mono cynomologus"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Carlos Clavel Claver <\/strong><\/h2>\n<p>Aislamiento y caracterizaci\u00f3n de c\u00e9lulas madre adultas pluripotenciales de medula osea humana y de mono cynomologus medula osea humana y de mono cynomologus #resumen: mapcs (multipotent adult progenitor cells) have been recently described as a population of adult pluripotent stem cells. We have successfully isolated and characterized a cell population from bone-marrow of adult human (hmapc) and adult cynomologus monkey (pmapc), that co-purify with mesenchymal stem cells. Pmapc proliferate over 150 pd and hmapc reached 60 pd, both without apparent senescence. H\/pmapc express stem cells markers (oct4, ssea4 and nanog) and lack expression of tissue stem cells specific antigens (cd44, class i and ii, cd31,cd34,cd45), demonstrating a phenotype consistent with human mapc described previously by dr. Verfaillie group. Within h\/pmapc populations, we can positively identify a \u00absmall   population of cells carrying high levies of self-renewal stem cell markers. In vitro, h\/pmapc differentiates into cells expressing markers of mesodermal derived tissues (bone, cartilage, muscle, endothelium) cells, neuroectoderm (neurons) and endoderm (hepatocytes). Also, no transformation or cytogenetic abnormalities has been observed. Interestingly, these populations of h\/pmapc can be isolated and identified without prolonged culture (as early as after 10-20 pd) allowing early expansion of cells. This cell population may have a great clinical and pre-clinical potential in cell therapy.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento y caracterizaci\u00f3n de c\u00e9lulas madre adultas pluripotenciales de m\u00e9dula \u00f3sea humana y de mono cynomologus<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento y caracterizaci\u00f3n de c\u00e9lulas madre adultas pluripotenciales de m\u00e9dula \u00f3sea humana y de mono cynomologus <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Carlos Clavel Claver <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 19\/09\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Felipe Luis Prosper Cardoso<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: agustin g. Zapata gonzalez <\/li>\n<li>Jos\u00e9 Carlos Segovia sanz (vocal)<\/li>\n<li>enrique Jos\u00e9 Andreu oltra (vocal)<\/li>\n<li>Mar\u00eda  consuelo Juan a Del ca\u00f1izo fernandez roldan (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Carlos Clavel Claver Aislamiento y caracterizaci\u00f3n de c\u00e9lulas madre adultas pluripotenciales de medula osea humana y de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,9399,17749],"tags":[164546,164545,164547,18255,84394,9401],"class_list":["post-76240","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-cultivo-celular","category-navarra","tag-agustin-g-zapata-gonzalez","tag-carlos-clavel-claver","tag-enrique-jose-andreu-oltra","tag-felipe-luis-prosper-cardoso","tag-jose-carlos-segovia-sanz","tag-maria-consuelo-juan-a-del-canizo-fernandez-roldan"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/76240","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=76240"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/76240\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=76240"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=76240"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=76240"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}