{"id":76337,"date":"2018-03-09T23:21:43","date_gmt":"2018-03-09T23:21:43","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/silenciamiento-genico-mediado-por-shrnas-en-celulas-del-epitelio-semina%c2%adfero-de-raton\/"},"modified":"2018-03-09T23:21:43","modified_gmt":"2018-03-09T23:21:43","slug":"silenciamiento-genico-mediado-por-shrnas-en-celulas-del-epitelio-semina%c2%adfero-de-raton","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/biologia-celular\/silenciamiento-genico-mediado-por-shrnas-en-celulas-del-epitelio-semina%c2%adfero-de-raton\/","title":{"rendered":"Silenciamiento g\u00e9nico mediado por shrnas en c\u00e9lulas del epitelio semin\u00edfero de rat\u00f3n"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Emilio Gonz\u00e1lez Gonz\u00e1lez <\/strong><\/h2>\n<p>Las espermatog\u00e9nesis es el proceso de diferenciaci\u00f3n terminal que conduce a la formaci\u00f3n de los espermatozoides. En la l\u00ednea germinal masculina, las espermatogonias proliferan y se diferencian a los restantes tipos celulares del epitelio de los t\u00fabulos semin\u00edferos del test\u00edculo: espermatocitos, esperm\u00e1tidas y espermatozoides. El proceso est\u00e1 coordinado por el componente som\u00e1tico del epitelio semin\u00edfero: las c\u00e9lulas de sertoli. en este trabajo, se ha evaluado el silenciamiento de la expresi\u00f3n g\u00e9nica mediante interferencia de rna (rnai), en los distintos tipos celulares del epitelio semin\u00edfero de rat\u00f3n (mus musculus). En el silenciamiento por rnai, mol\u00e9culas de rna de doble cadena son capaces de promover represi\u00f3n post-transcripcional de un gen diana por complementariedad de secuencia.  en las c\u00e9lulas del epitelio semin\u00edfero de ratones transg\u00e9nicos que expresan el gen egfp, se introdujeron vectores de dna que permit\u00edan la expresi\u00f3n de peque\u00f1os rnas, capaces de constituirse en rnas de doble cadena en horquilla (shrnas), con potencialidad de guiar el silenciamiento especifico del gen egfp.  la transfecci\u00f3n de los vectores en las c\u00e9lulas del epitelio semin\u00edfero se llev\u00f3 a cabo in vivo, mediante electroporaci\u00f3n por onda cuadrada, e in vitro, mediante lipofecci\u00f3n. en ambos casos se analiz\u00f3 la eficacia de transfecci\u00f3n del vector utilizado y la eficiencia del silenciamiento. Tambi\u00e9n, en este trabajo, se ha llevado a cabo la caracterizaci\u00f3n, utilizando la t\u00e9cnica de rt-pcr en tiempo real, de la expresi\u00f3n de los genes drosha, dicer y ago 1-4; genes que codifican prote\u00ednas implicadas en el proceso de interferencia de rna. El estudio se ha realizado valorando comparativamente los niveles de expresi\u00f3n g\u00e9nica durante el desarrollo postnatal del test\u00edculo, en las c\u00e9lulas aisladas del epitelio seminifero y en diferentes tejidos som\u00e1ticos adultos. los resultados obtenidos en esta tesis doctoral muestran que, utilizando la metodolog\u00eda de<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Silenciamiento g\u00e9nico mediado por shrnas en c\u00e9lulas del epitelio semin\u00edfero de rat\u00f3n<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Silenciamiento g\u00e9nico mediado por shrnas en c\u00e9lulas del epitelio semin\u00edfero de rat\u00f3n <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Emilio Gonz\u00e1lez Gonz\u00e1lez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 25\/09\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Mazo Mart\u00ednez Jes\u00fas Del<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Mar\u00eda  Jes\u00fas Puertas gallego <\/li>\n<li> L\u00f3pez fern\u00e1ndez Luis Andr\u00e9s (vocal)<\/li>\n<li> L\u00f3pez ala\u00f1\u00f3n dulce Mar\u00eda (vocal)<\/li>\n<li>  (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Emilio Gonz\u00e1lez Gonz\u00e1lez Las espermatog\u00e9nesis es el proceso de diferenciaci\u00f3n terminal que conduce a la formaci\u00f3n de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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