{"id":76765,"date":"2018-03-09T23:22:11","date_gmt":"2018-03-09T23:22:11","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estudio-del-desensamblaje-in-vitro-y-analisis-estructura-funcion-de-cavidades-de-la-capsida-del-virus-diminuto-del-raton\/"},"modified":"2018-03-09T23:22:11","modified_gmt":"2018-03-09T23:22:11","slug":"estudio-del-desensamblaje-in-vitro-y-analisis-estructura-funcion-de-cavidades-de-la-capsida-del-virus-diminuto-del-raton","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/estudio-del-desensamblaje-in-vitro-y-analisis-estructura-funcion-de-cavidades-de-la-capsida-del-virus-diminuto-del-raton\/","title":{"rendered":"Estudio del desensamblaje in vitro y analisis estructura funcion de cavidades de la capsida del virus diminuto del raton"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Aura Carreira Moreno <\/strong><\/h2>\n<p>Las c\u00e1psidas de virus esf\u00e9ricos son grandes prote\u00ednas multim\u00e9ricas y constituyen un modelo atractivo para el estudio de la asociaci\u00f3n, estabilidad y desensamblaje de prote\u00ednas complejas. Adem\u00e1s, proporcionan una oportunidad para entender la relaci\u00f3n estructura-funci\u00f3n de las part\u00edculas virales, y en \u00faltimo t\u00e9rmino, facilitan el desarrollo de vacunas m\u00e1s estables y de nuevas estrategias antivirales basadas en la inhibici\u00f3n del ensamblaje o la desencapsidaci\u00f3n. en la presente tesis doctoral hemos analizado el desensamblaje de la c\u00e1psida de mvm in vitro y hemos llevado a cabo un an\u00e1lisis estructura-funci\u00f3n del papel de cavidades y una carga enterrada situada en el n\u00facleo hidrof\u00f3bico de cada subunidad de la c\u00e1psida en el ensamblaje y estabilidad de la c\u00e1psida, y en la funcionalidad del virus. Los resultados espec\u00edficos obtenidos se pueden resumir de la siguiente manera:  1. Los an\u00e1lisis biof\u00edsicos y bioqu\u00edmicos del desensamblaje de la c\u00e1psida de mvm realizados in vitro han revelado una ruta compleja que puede implicar varias etapas: un reordenamiento conformacional reversible, asociado pero no limitado a la externalizaci\u00f3n del extremo n-terminal de un n\u00famero de subunidades de la c\u00e1psida; la disociaci\u00f3n irreversible de la c\u00e1psida en un intermediario; y la disociaci\u00f3n\/desnaturalizaci\u00f3n de este en una forma del mon\u00f3mero con estructura similar a la de un gl\u00f3bulo fundido.  2. La determinaci\u00f3n de la fluorescencia intr\u00ednseca de los tript\u00f3fanos de la temperatura de transici\u00f3n del paso espec\u00edfico de disociaci\u00f3n de la c\u00e1psida se ha validado como procedimiento fiable y preciso para analizar el efecto de mutaciones o condiciones en la estabilidad relativa de la c\u00e1psida de mvm frente a la disociaci\u00f3n por calor.  3. La inserci\u00f3n de ep\u00edtopos heter\u00f3logos en cuatro bucles expuestos al disolvente de la superficie de la c\u00e1psida de mvm, a pesar de encontrarse en los sitios presumiblemente m\u00e1s permisivos de la c<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estudio del desensamblaje in vitro y analisis estructura funcion de cavidades de la capsida del virus diminuto del raton<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estudio del desensamblaje in vitro y analisis estructura funcion de cavidades de la capsida del virus diminuto del raton <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Aura Carreira Moreno <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 20\/10\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Mauricio Garcia Mateu<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Lopez carrascosa <\/li>\n<li>margarita Menendez (vocal)<\/li>\n<li>Antonio Llamas saiz (vocal)<\/li>\n<li>Luis Menendez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Aura Carreira Moreno Las c\u00e1psidas de virus esf\u00e9ricos son grandes prote\u00ednas multim\u00e9ricas y constituyen un modelo atractivo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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