{"id":76783,"date":"2018-03-09T23:22:11","date_gmt":"2018-03-09T23:22:11","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-molecular-e-inmunologica-del-anta%c2%adgeno-recombinante-rhcp26-23-de-haemonchus-contortus-ensayos-vacunales\/"},"modified":"2018-03-09T23:22:11","modified_gmt":"2018-03-09T23:22:11","slug":"caracterizacion-molecular-e-inmunologica-del-anta%c2%adgeno-recombinante-rhcp26-23-de-haemonchus-contortus-ensayos-vacunales","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-molecular-e-inmunologica-del-anta%c2%adgeno-recombinante-rhcp26-23-de-haemonchus-contortus-ensayos-vacunales\/","title":{"rendered":"Caracterizaci\u00f3n molecular e inmunol\u00f3gica del ant\u00edgeno recombinante rhcp26\/23 de haemonchus contortus. ensayos vacunales"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Leticia Garc\u00eda Coiradas <\/strong><\/h2>\n<p>Las gastroenteritis parasitarias por helmintos constituyen una de las enfermedades parasitarias m\u00e1s frecuentes del ganado ovino a nivel mundial. Dentro de estos procesos se encuentra la hemoncosis ovina, enfermedad causada por el nematodo g\u00e1strico h.Contortus, responsable de numerosas p\u00e9rdidas econ\u00f3micas. el control de este proceso ha radicado, casi exclusivamente, en el uso de f\u00e1rmacos antihelm\u00ednticos; sin embargo su uso indiscriminado, y en ocasiones no relacionado con los procesos, ha ocasionado la aparici\u00f3n de resistencias frente a los principales principios activos. Ante esta situaci\u00f3n la alternativa es la inmunoprofilaxis. Son numerosos los ant\u00edgenos que han mostrado eficacia frente a h.Contortus, si bien la dificultad en la obtenci\u00f3n de cantidades suficientes de estos hace que sea necesario recurrir a nuevas tecnolog\u00edas -adn recombinante- para obtener ant\u00edgeno en cantidades abundantes que permitan disponer de una vacuna comercial, sin embargo, hasta el momento ninguno de los ant\u00edgenos recombinantes probados han mostrado la misma eficiencia que su hom\u00f3logo natural.     la fracci\u00f3n antig\u00e9nica p26\/23 es una de las que han mostrado su poder inmunoprotector frente a h.Contortus, y dado que su hom\u00f3logo recombinante no hab\u00eda sido probado nos propusimos el aislamiento, purificaci\u00f3n y caracterizaci\u00f3n de la prote\u00edna p26\/23, su secuenciaci\u00f3n, clonaci\u00f3n y expresi\u00f3n en e.Coli (rhcp26\/23), tanto en condiciones nativas como desnaturalizantes, valorando su poder inmunoprotector en un ensayo vacunal con corderos de 3 meses de edad.     a partir de membranas de nylon electrotransferidas con esa se escindieron los p\u00e9ptidos de 24 kda (p26\/23), tras su identificaci\u00f3n con sueros de ovejas vacunadas en experimentos anteriores. El producto as\u00ed obtenido fue sometido a cromatograf\u00eda de fase reversa (rp-hplc), eliminando posibles contaminaciones con p\u00e9ptidos comigrantes, obteniendo un \u00fanico p\u00e9ptido purificado de ca. 24kda (p26\/23). El p\u00e9ptido obteni<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n molecular e inmunol\u00f3gica del ant\u00edgeno recombinante rhcp26\/23 de haemonchus contortus. ensayos vacunales<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n molecular e inmunol\u00f3gica del ant\u00edgeno recombinante rhcp26\/23 de haemonchus contortus. ensayos vacunales <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Leticia Garc\u00eda Coiradas <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 21\/10\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Alunda Rodr\u00edguez Jos\u00e9 Mar\u00eda<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio  ramon Martinez fernandez <\/li>\n<li>basilio Valladares hern\u00e1ndez (vocal)<\/li>\n<li>Francisco Antonio Rojo vazquez (vocal)<\/li>\n<li>vicente Larraga rodr\u00edguez de vera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Leticia Garc\u00eda Coiradas Las gastroenteritis parasitarias por helmintos constituyen una de las enfermedades parasitarias m\u00e1s frecuentes del [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[7383,664,35395,3216,165567,54495],"class_list":["post-76783","post","type-post","status-publish","format-standard","hentry","category-sin-categoria","tag-alunda-rodriguez-jose-maria","tag-antonio-ramon-Martinez-fernandez","tag-basilio-valladares-hernandez","tag-francisco-antonio-rojo-vazquez","tag-leticia-garcia-coiradas","tag-vicente-larraga-rodriguez-de-vera"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/76783","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=76783"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/76783\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=76783"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=76783"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=76783"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}