{"id":77544,"date":"2005-01-12T00:00:00","date_gmt":"2005-01-12T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/optimizacion-del-sistema-tet-on-para-regular-la-expresion-de-inteleucina-12-en-el-ha%c2%adgado-y-su-aplicacion-al-tratamiento-de-tumores-hepaticos\/"},"modified":"2005-01-12T00:00:00","modified_gmt":"2005-01-12T00:00:00","slug":"optimizacion-del-sistema-tet-on-para-regular-la-expresion-de-inteleucina-12-en-el-ha%c2%adgado-y-su-aplicacion-al-tratamiento-de-tumores-hepaticos","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/inmunologia\/optimizacion-del-sistema-tet-on-para-regular-la-expresion-de-inteleucina-12-en-el-ha%c2%adgado-y-su-aplicacion-al-tratamiento-de-tumores-hepaticos\/","title":{"rendered":"Optimizaci\u00f3n del sistema tet-on para regular la expresi\u00f3n de inteleucina-12 en el h\u00edgado y su aplicaci\u00f3n al tratamiento de tumores hep\u00e1ticos"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Maider Zabala Ugalde <\/strong><\/h2>\n<p>Titulo: optimizaci\u00f3n del sistema tet-on para regular la expresi\u00f3n de interleucina-12 en el h\u00edgado y su aplicaci\u00f3n al tratamiento de tumores hep\u00e1ticos resumen: optimization of the tet-on system to regulate interleukin-12 expression in the liver for the treatment of hepatic tumors maider zabala uqalde faculty of science, university of navarra (spain) 2005 hepatocellular carcinoma (hcc) is a prevalent form of cancer, which lacks of curative treatment in most patients. For that reason there is an urgent need for new and efficient treatments. One experimental approach consists on interleukin-12 (il-12)-based gene therapy. This cytokine has a potent antitumoral activity but it can be toxic at high doses. In order to minimize its side effects we have generated a plasmid vector able to direct expression of murine il-12 in a liver-specific and doxycycline (dox)-inducible manner. The plasmid was transferred to the liver by the hydrodynamics-based procedure and the antitumor efficacy of il-12 was evaluated in two animal models: (i) met\u00e1stasis of colon cancer to the liver and (ii) hcc developed in transgenic mice. In the first case 100% of anim\u00e1is showed complete tumor regression after 10 days of mll-12 expression. Transgenic pk\/c-myc mice over-express c-myc in the liver and develop tumors in 6-8 months that resemble human hcc in terms of biology and gene expression profile. After several rounds of mll-12 induction during 168 days, we observed an inhibition of tumor growth and survival of 40% of treated mice. These data showed the great potential of il-12 for the treatment of metastatic and primary liver cancer, we have also aimed at improving the non-invasive detection of hcc using the pk\/c-myc mice and different pet tracers. We found that combination of 18fdg and 11c-choline analysis allowed detection of up to 78% of tumor lesions<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Optimizaci\u00f3n del sistema tet-on para regular la expresi\u00f3n de inteleucina-12 en el h\u00edgado y su aplicaci\u00f3n al tratamiento de tumores hep\u00e1ticos<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Optimizaci\u00f3n del sistema tet-on para regular la expresi\u00f3n de inteleucina-12 en el h\u00edgado y su aplicaci\u00f3n al tratamiento de tumores hep\u00e1ticos <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Maider Zabala Ugalde <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Navarra<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/12\/2005<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Gabriela Kramer<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jes\u00fas m. Prieto valtue\u00f1a <\/li>\n<li>Antonio Bernad mia\u00f1a (vocal)<\/li>\n<li>marcos Malumbres mart\u00ednez (vocal)<\/li>\n<li>Jos\u00e9 Yelamos lopez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Maider Zabala Ugalde Titulo: optimizaci\u00f3n del sistema tet-on para regular la expresi\u00f3n de interleucina-12 en el h\u00edgado [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[36483,9399,1737,17749],"tags":[51006,166976,15059,118897,166975,53260],"class_list":["post-77544","post","type-post","status-publish","format-standard","hentry","category-biologia-molecular","category-cultivo-celular","category-inmunologia","category-navarra","tag-antonio-bernad-miana","tag-gabriela-kramer","tag-jesus-m-prieto-valtuena","tag-jose-yelamos-lopez","tag-maider-zabala-ugalde","tag-marcos-malumbres-Martinez"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/77544","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=77544"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/77544\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=77544"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=77544"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=77544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}