{"id":78177,"date":"2006-09-01T00:00:00","date_gmt":"2006-09-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/desarrollo-y-evaluacion-de-un-metodo-de-diagnostico-de-micosis-profundas-mediante-tecnicas-de-biologia-molecular\/"},"modified":"2006-09-01T00:00:00","modified_gmt":"2006-09-01T00:00:00","slug":"desarrollo-y-evaluacion-de-un-metodo-de-diagnostico-de-micosis-profundas-mediante-tecnicas-de-biologia-molecular","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-medicas\/desarrollo-y-evaluacion-de-un-metodo-de-diagnostico-de-micosis-profundas-mediante-tecnicas-de-biologia-molecular\/","title":{"rendered":"Desarrollo y evaluacion de un metodo de diagnostico de micosis profundas mediante tecnicas de biolog\u00eda molecular"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Alejandro Jover Botella <\/strong><\/h2>\n<p>La incidencia de las infecciones f\u00fangicas invasivas (ifis) ha ido aumentando de manera notable en las \u00faltimas d\u00e9cadas. El motivo principal es que cada vez son m\u00e1s los pacientes con alg\u00fan tipo de inmunosupresi\u00f3n o con distintos niveles de compromiso inmunol\u00f3gico.  en la actualidad muchos esfuerzos se centran en el desarrollo de nuevas herramientas de diagn\u00f3stico basadas en la detecci\u00f3n de \u00e1cidos nucleicos m\u00e1s sensibles, espec\u00edficas y r\u00e1pidas que las t\u00e9cnicas convencionales de microscop\u00eda, cultivo e inmunolog\u00eda principalmente m\u00e9todos moleculares basados en la detecci\u00f3n de \u00e1cidos nucleicos, campo en el que se han alcanzado grandes avances. En este trabajo hemos optimizado y evaluado un m\u00e9todo r\u00e1pido y sensible para la detecci\u00f3n de hongos en muestras cl\u00ednicas basado en la amplificaci\u00f3n mediante pcr semi-anidada con posterior secuenciaci\u00f3n del dna.  para ello empleamos tres regiones distintas del complejo ribos\u00f3mico que nos permitieron realizar diagn\u00f3stico molecular de ifis en base a dos estrategias diferentes de detecci\u00f3n de hongos: identificaci\u00f3n orientada, empleando cebadores espec\u00edficos para aspergillus spp (afu7\/afu5) y candida albicans (ctsr\/cadet\/ctsf), e identificaci\u00f3n no orientada mediante cebadores panf\u00fangicos (its1\/its4\/its86).  en primer lugar se optimizaron para todos estos cebadores sus reacciones de amplificaci\u00f3n con el fin de obtener la mayor sensibilidad posible ensayando diferentes concentraciones de cebadores y n\u00famero de ciclos. A continuaci\u00f3n cuantificamos la sensibilidad de nuestro m\u00e9todo diagn\u00f3stico mediante un ensayo en agua y en distintos tejidos humanos, permiti\u00e9ndonos comprobar su utilidad en la detecci\u00f3n de hongos en diferentes tipos de muestra cl\u00ednica (sangre, l\u00edquido cefalorraqu\u00eddeo, l\u00edquido asc\u00edtico, l\u00edquido pleural y lavado broncoalveolar). La sensibilidad de la t\u00e9cnica para c. Albicans fue de 1 c\u00e9lula\/500 \u00c2\u00b5l con los cebadores its1\/its86\/its4 en todos los tejidos<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Desarrollo y evaluacion de un metodo de diagnostico de micosis profundas mediante tecnicas de biolog\u00eda molecular<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Desarrollo y evaluacion de un metodo de diagnostico de micosis profundas mediante tecnicas de biolog\u00eda molecular <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Alejandro Jover Botella <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Miguel hern\u00e1ndez de elche<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 09\/01\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Consuelo Ferrer Rodr\u00edguez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: jose Ponton sanemeterio <\/li>\n<li>Mar\u00eda no Andreu lopez (vocal)<\/li>\n<li>Javier Peman garcia (vocal)<\/li>\n<li> Moragues tosantos Mar\u00eda  dolores (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Alejandro Jover Botella La incidencia de las infecciones f\u00fangicas invasivas (ifis) ha ido aumentando de manera notable 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