{"id":78287,"date":"2018-03-09T23:23:54","date_gmt":"2018-03-09T23:23:54","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-sistema-de-aciltransferasas-en-semillas-de-girasol-helianthus-annuus-l-clonacion-de-una-ferredoxina-heterotrofica\/"},"modified":"2018-03-09T23:23:54","modified_gmt":"2018-03-09T23:23:54","slug":"caracterizacion-del-sistema-de-aciltransferasas-en-semillas-de-girasol-helianthus-annuus-l-clonacion-de-una-ferredoxina-heterotrofica","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/bioquimica-molecular\/caracterizacion-del-sistema-de-aciltransferasas-en-semillas-de-girasol-helianthus-annuus-l-clonacion-de-una-ferredoxina-heterotrofica\/","title":{"rendered":"Caracterizaci\u00f3n del sistema de aciltransferasas en semillas de girasol (helianthus annuus l.). clonaci\u00f3n de una ferredoxina heterotr\u00f3fica"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Noem\u00ed Ruiz L\u00f3pez <\/strong><\/h2>\n<p>Los objetivos de esta tesis doctoral fueron:  primero     desarrollar un m\u00e9todo anal\u00edtico de extracci\u00f3n r\u00e1pida de aceite a partir de media semilla, con el fin de determinar con precisi\u00f3n la composici\u00f3n de especies moleculares de triacilglic\u00e9ridos en el aceite de media semilla de girasol por cromatograf\u00eda gas-l\u00edquido.  segundo     definir un coeficiente matem\u00e1tico que indique la distribuci\u00f3n de \u00e1cidos grasos saturados entre las posiciones sn-1 y sn-3 del triacilglic\u00e9rido.  tercero     caracterizaci\u00f3n bioqu\u00edmica de las actividades aciltransferasas (gpat y lpaat) microsomales de girasol, responsables de la s\u00edntesis de tags y por tanto, de la proporci\u00f3n final de las distintas especies de tags en un aceite de girasol.  cuarto     purificaci\u00f3n parcial de la gpat con el fin de conseguir su secuencia anminoac\u00eddica.  quinto     clonaci\u00f3n y secuenciaci\u00f3n del donador de electrones de desaturasas plastidiales, la ferredoxina de girasol.  las conclusiones obtenidas fueron:     1,- se ha desarrollado un m\u00e9todo r\u00e1pido de extracci\u00f3n de triacilglic\u00e9ridos (tags), basado en la extracci\u00f3n de aceites de tejidos vegetales, utilizando una mezcla compuesta por un 33,3% de metanol con nacl (0,17 m) y un 66,6% de heptano en volumen.     2,- la extracci\u00f3n \u00f3ptima de los tags, de una muestra vegetal, se consigui\u00f3 tras una incubaci\u00f3n de esta muestra en la mezcla durante 2 horas a 80\u00c2\u00bac. La composici\u00f3n de tags obtenida es representativa del total de los encontrados en el tejido vegetal. Los tags estadios pueden ser posteriormente metilados para obtener la composici\u00f3n en \u00e1cidos grasos, permitiendo el an\u00e1lisis de sus \u00e9steres met\u00edlicos en la misma muestra de tejido.     3,- este m\u00e9todo permite la extracci\u00f3n de tags de un gran n\u00famero de muestras vegetales de peque\u00f1o tama\u00f1o. Y por ello, es muy \u00fatil para la selecci\u00f3n de nuevas variedades vegetales, teniendo en cuenta la composici\u00f3n en los tags y \u00e1cidos grasos de las semillas y fru<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizaci\u00f3n del sistema de aciltransferasas en semillas de girasol (helianthus annuus l.). clonaci\u00f3n de una ferredoxina heterotr\u00f3fica<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizaci\u00f3n del sistema de aciltransferasas en semillas de girasol (helianthus annuus l.). clonaci\u00f3n de una ferredoxina heterotr\u00f3fica <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Noem\u00ed Ruiz L\u00f3pez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Sevilla<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 18\/01\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Enrique Mart\u00ednez Force<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Jos\u00e9 M\u00aa Maldonado ruiz <\/li>\n<li>Jos\u00e9 Mart\u00ednez fern\u00e1ndez (vocal)<\/li>\n<li> Slas li\u00f1\u00e1n joaqu\u00edn Jes\u00fas (vocal)<\/li>\n<li>Manuel Santiago Alaiz barrag\u00e1n (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Noem\u00ed Ruiz L\u00f3pez Los objetivos de esta tesis doctoral fueron: primero desarrollar un m\u00e9todo anal\u00edtico de extracci\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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