{"id":80063,"date":"2018-03-09T23:25:58","date_gmt":"2018-03-09T23:25:58","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/metabolismo-del-acetato-en-phycomyces-blakesleeanus-purificacion-y-caracterizacion-de-dos-acetil-coa-sintetasas\/"},"modified":"2018-03-09T23:25:58","modified_gmt":"2018-03-09T23:25:58","slug":"metabolismo-del-acetato-en-phycomyces-blakesleeanus-purificacion-y-caracterizacion-de-dos-acetil-coa-sintetasas","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/metabolismo-intermediario\/metabolismo-del-acetato-en-phycomyces-blakesleeanus-purificacion-y-caracterizacion-de-dos-acetil-coa-sintetasas\/","title":{"rendered":"Metabolismo del acetato en phycomyces blakesleeanus. purificaci\u00f3n y caracterizaci\u00f3n de dos acetil-coa sintetasas"},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Cima Martin Sergio De <\/strong><\/h2>\n<p>Phycomyces blakesleeanus es un hongo filamentoso capaz de crecer en acetato, para lo cual necesita la enzima acetil-coa sintetasa (acs1), codificada por el gen faca, que es inducido por acetato y reprimido por glucosa. Hemos detectado en situaciones de estr\u00e9s por ausencia de fuente de carbono la existencia de una segunda acetil-coa sintetasa (acs2), que no es codificada por el gen faca y no contribuye al crecimiento del organismo en acetato. Ambas acs han sido purificadas y caracterizadas, siendo sus propiedades cin\u00e9ticas similares entre ellas. Las dos enzimas pueden activar propionato adem\u00e1s de acetato, muestran una cin\u00e9tica de saturaci\u00f3n hiperb\u00f3lica con respecto a acetato, propionato, coenzima a y atp, y tienen un ph \u00f3ptimo en torno a 8,0. Ninguno de los metabolitos intermediarios estudiados tuvo alg\u00fan efecto inh\u00edbidor o activador sobre ninguna de las dos enzimas. Por otro lado, la inhibici\u00f3n por nem y anh\u00eddrido succ\u00ednico sugiere la participaci\u00f3n de al menos un residuo de ciste\u00edna y un residuo de lisina en el centro activo y\/o en el mantenimiento de la estructura nativa de ambas enzimas. La diferencia m\u00e1s notable encontrada entre ambas enzimas es su diferente temperatura \u00f3ptima: 30\u00c2\u00bac para acs1 y 50\u00c2\u00bac para acs2, temperatura esta \u00faltima muy superior a la temperatura de crecimiento del hongo. Tambi\u00e9n la estabilidad frente a temperatura, ph, urea y digesti\u00f3n por  tripsina es mayor en acs2 que en acs1, lo que apoya la teor\u00eda de que acs2 tiene una mayor rigidez conformacional y pueda ser considerada una prote\u00edna de estr\u00e9s.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Metabolismo del acetato en phycomyces blakesleeanus. purificaci\u00f3n y caracterizaci\u00f3n de dos acetil-coa sintetasas<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Metabolismo del acetato en phycomyces blakesleeanus. purificaci\u00f3n y caracterizaci\u00f3n de dos acetil-coa sintetasas <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Cima Martin Sergio De <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Le\u00f3n<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/05\/2006<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>M\u00aa Dolores Arriaga Giner<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: angel Reglero chillon <\/li>\n<li>Juan  Jos\u00e9 Arag\u00f3n reyes (vocal)<\/li>\n<li>Fernando Moreno sanz (vocal)<\/li>\n<li>mercedes Renobales scheifler (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Cima Martin Sergio De Phycomyces blakesleeanus es un hongo filamentoso capaz de crecer en acetato, para lo [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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