{"id":80269,"date":"2018-03-10T00:03:59","date_gmt":"2018-03-10T00:03:59","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/caracterizacion-del-retrovirus-asociado-con-el-tumor-intranasal-enzootico-de-la-oveja\/"},"modified":"2018-03-10T00:03:59","modified_gmt":"2018-03-10T00:03:59","slug":"caracterizacion-del-retrovirus-asociado-con-el-tumor-intranasal-enzootico-de-la-oveja","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/caracterizacion-del-retrovirus-asociado-con-el-tumor-intranasal-enzootico-de-la-oveja\/","title":{"rendered":"Caracterizacion del retrovirus asociado con el tumor intranasal enzootico de la oveja."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Esmeralda Minguijon Perez <\/strong><\/h2>\n<p>El tumor intranasal enzo\u00f3tico de la oveja es una enfermedad neopl\u00e1sica transmisible. La transmisi\u00f3n experimental del proceso estableci\u00f3 un posible v\u00ednculo de la neoplasia con un agente infeccioso. Posteriormente los estudios de microscop\u00eda electr\u00f3nica demostraron la presencia de part\u00edculas retrovirales en relaci\u00f3n con c\u00e9luas tumorales en animales seronegativos a lentivirus. as\u00ed mismo fue detectada, en extractos de tejido tumoral y fluido nasal, la existencia de actividad enzim\u00e1tica transcriptasa inversa en fracciones de densidad caracter\u00edsticas de retrovirus. En estas mismas fracciones se demostr\u00f3 la presencia de polip\u00e9ptidos que reaccionaban cruzadamente con otros retrovirus de tipo d y b como son el mason pfizer monkey virus (mpmv) y el virus de la adenomatosis pulmonar ovina (jsrv). Conocidas estas relaciones con otros retrovirus y teniendo en cuenta la imposibilidad de propagar el virus en sistemas celulares y la existencia de numerosos retrovirus end\u00f3genos ovinos similares a jsrv, y presumiblemente al retrovirus relacionado con el tumor intranasal enzo\u00f3tico de la oveja (oventv), procedimos caracterizar este agente empleando para ello t\u00e9cnicas de biolog\u00eda molecular. Para ello en primer lugar se emplearon distintos protocolos de pcr desarrollados para la detecci\u00f3n de jsrv obteniendo producto amplificado de un fragmento del gen gag. Al clonar y secuenciar dicho producto se comprob\u00f3 que en esa regi\u00f3n oventv era diferente de jsrv y de las copias retrovirales end\u00f3genas y se demostr\u00f3 la existencia de dos enzimas restricci\u00f3n aatii y psti que pod\u00edan ser empleadas como marcadores moleculares de oventv. Una vez comprobada la diferente naturaleza de los agentes se procedi\u00f3 a clonar y caracterizar el genoma completo de oventv. para ello se sintetizaron dos genotecas en las cuales se realizaron distintos cribados empleados diferentes sondas marcadas radioactivamente procedentes de diversos clones de jsrv. o<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Caracterizacion del retrovirus asociado con el tumor intranasal enzootico de la oveja.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Caracterizacion del retrovirus asociado con el tumor intranasal enzootico de la oveja. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Esmeralda Minguijon Perez <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Zaragoza<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 30\/06\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Heras Guillamon Marcelo De Las<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: diego Dualde perez <\/li>\n<li>james Sharp michael (vocal)<\/li>\n<li> Garcia de jalon ciercoles Jos\u00e9 Antonio (vocal)<\/li>\n<li>Antonio Contreras de vera (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Esmeralda Minguijon Perez El tumor intranasal enzo\u00f3tico de la oveja es una enfermedad neopl\u00e1sica transmisible. La transmisi\u00f3n [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[494,35,515,6956,280,3030,13610],"tags":[43701,5216,171989,28850,119322,171990],"class_list":["post-80269","post","type-post","status-publish","format-standard","hentry","category-ciencias-agrarias","category-ciencias-de-la-vida","category-ciencias-veterinarias","category-retrovirus","category-virologia","category-virologia-veterinaria","category-zaragoza","tag-antonio-contreras-de-vera","tag-diego-dualde-perez","tag-esmeralda-minguijon-perez","tag-garcia-de-jalon-ciercoles-jose-antonio","tag-heras-guillamon-marcelo-de-las","tag-james-sharp-michael"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/80269","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=80269"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/80269\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=80269"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=80269"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=80269"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}