{"id":81746,"date":"2018-03-10T00:05:37","date_gmt":"2018-03-10T00:05:37","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/metodos-moleculares-de-estudio-de-las-poblaciones-de-pseudomonas-stutzeri\/"},"modified":"2018-03-10T00:05:37","modified_gmt":"2018-03-10T00:05:37","slug":"metodos-moleculares-de-estudio-de-las-poblaciones-de-pseudomonas-stutzeri","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/metodos-moleculares-de-estudio-de-las-poblaciones-de-pseudomonas-stutzeri\/","title":{"rendered":"Metodos moleculares de estudio de las poblaciones de pseudomonas stutzeri."},"content":{"rendered":"<h2>Tesis doctoral de <strong>  Guasp Mascaro Caterina M. <\/strong><\/h2>\n<p>Pseudomonas stutzari es un microorganismo com\u00fan en diveros h\u00e1bitats y ha recibido una especial atenci\u00f3n debido a sus especiales capacidades metab\u00f3licas como degradador de compuestos arom\u00e1ticos (garcia-vald\u00e9s et al.,1988). Se han dise\u00f1ado m\u00e9todos moleculares que permiten la identificai\u00f3n y el seguimiento de p. Stutzari en muestras naturales. La identificai\u00f3n de p. Stutzaeri se consigui\u00f3 utilizando cebadores espec\u00edficos para amplificar el gen rdna 16s seguido por el an\u00e1lisis rflp (\u00abrestrction frament length polymorphism\u00bb) al tratar los productos de pcr con la endonucleasa bamhi. Por otra parte, se han utilizado m\u00e9todos molecualres de tipado basados en la amplificaic\u00f3n por pcr con cebadores para secuencias consenso derivadas de regiones invertidas repetidas palindr\u00f3micas altamente conservadas encontradas en enterobacterias (eric). Finalmente, se ha utilizado un m\u00e9todo de western blot y \u00abfingerprinting\u00bb inmunol\u00f3gico para la diferenciaci\u00f3n de las cepas de p. Stutzeri. La asignaci\u00f3n de las cepas a una genomovar determinada se ha conseguido de una manera r\u00e1pida y f\u00e1cil mediante amplifiaci\u00f3n por pcr de las regiones espaciadoras intrg\u00e9nicas 16s-23s rdna (isr) y su poserior an\u00e1lisis por rflp tras la digesti\u00f3n con el enzima de restricci\u00f3n taqi. Adem\u00e1s, se han secuenciado estas regiones y, despu\u00e9s de su an\u00e1lisis, se ha comprobado que los grupos obtenidos conciden perfectamente con cada una de las genomovares de p. stutzeri. Se han utilizado las t\u00e9cnicas de ecolog\u00eda microbiana molecular para hacer un seguimiento de la cepa degradadora de naftaleno an10 en la biorremediaci\u00f3n en un modelo experimental de microcosmos de sedimiento marino contaminados con salcilato, naftaleno o queroseno jeta1. Para ello se ha utilizado el an\u00e1lisis por dgge de dos genes, el rrna 16s y el gen catab\u00f3lico nhac, involucrado en la oxidaci\u00f3n inicial del naftaleno a 1,2 dihidroxinaftaleno.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Metodos moleculares de estudio de las poblaciones de pseudomonas stutzeri.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Metodos moleculares de estudio de las poblaciones de pseudomonas stutzeri. <\/li>\n<li><strong>Autor:<\/strong>\u00a0  Guasp Mascaro Caterina M. <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Illes balears<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 17\/12\/1999<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jordi Lalucat Jo<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: alberto Ramos cormenzana <\/li>\n<li>Jes\u00fas Lopez romalde (vocal)<\/li>\n<li>Francisco Rodriguez valera (vocal)<\/li>\n<li>r.b. Moore edward (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Guasp Mascaro Caterina M. Pseudomonas stutzari es un microorganismo com\u00fan en diveros h\u00e1bitats y ha recibido una [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[35,19034,278],"tags":[17946,19239,174270,63339,251,174271],"class_list":["post-81746","post","type-post","status-publish","format-standard","hentry","category-ciencias-de-la-vida","category-illes-balears","category-microbiologia","tag-alberto-ramos-cormenzana","tag-francisco-rodriguez-valera","tag-guasp-mascaro-caterina-m","tag-jesus-lopez-romalde","tag-jordi-lalucat-jo","tag-r-b-moore-edward"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/81746","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=81746"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/81746\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=81746"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=81746"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=81746"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}