{"id":82802,"date":"2000-01-01T00:00:00","date_gmt":"2000-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/estructura-tridimensional-del-domini-receptor-del-factor-de-transcripcio-phob\/"},"modified":"2000-01-01T00:00:00","modified_gmt":"2000-01-01T00:00:00","slug":"estructura-tridimensional-del-domini-receptor-del-factor-de-transcripcio-phob","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/estructura-tridimensional-del-domini-receptor-del-factor-de-transcripcio-phob\/","title":{"rendered":"Estructura tridimensional del domini receptor del factor de transcripcio phob."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Mar\u00eda Sol\u00c1\u00a0 Vilarrubias <\/strong><\/h2>\n<p>Para ampliar el estudio de prote\u00ednas transductoras de se\u00f1al de dos componentes iniciado en el laboratorio y, a la vez, mantenernos en el marco de las prote\u00ednas que interact\u00faan con el dna, escogimos el factor de transcripci\u00f3n phob, que es una prote\u00edna bacteriana reguladora de respuesta. as\u00ed, se decidi\u00f3 amplificar y clonar el segmento de dna del gen phob correspondiente al dominio receptor del fosfato (residuos del 1 al 125), expresar este dominio y, posteriormente, purificarlo en grado suficiente para facilitar la futura obtenci\u00f3n de cristales. M\u00e1s tarde se buscar\u00edan condiciones de cristalizaci\u00f3n para obtener cristales de calidad suficiente para su ulterior difracci\u00f3n empleando rayos x. Con los datos de difracci\u00f3n se determinar\u00eda la estructura tridimensional de la prote\u00edna, la cual posteriormente, se afinar\u00eda, verificar\u00eda y, finalmente, se interpretar\u00eda la estructura cristalogr\u00e1fica. Se lograron los objetivos planteados, consigui\u00e9ndose cristales nativos y cristales derivados con un \u00e1tomo de oro. Con \u00e9stos \u00faltimos se realiz\u00f3 un experimento de difracci\u00f3n an\u00f3mala a m\u00faltiples longitudes de onda, que sirvi\u00f3 para obtener unas fases iniciales y calcular un mapa de densidad electr\u00f3nica, con el que se traz\u00f3 el modelo. Mediante remplazamiento molecular usando este modelo se resolvieron los datos de difracci\u00f3n del cristal nativo (que llegan hasta 1.9 a de resoluci\u00f3n), cuya estructura se afin\u00f3 y verific\u00f3. la determinaci\u00f3n de la estructura tridimensional del dominio receptor del fosfato revela un plegamiento de doble vuelta (alfabeta)5. En el centro activo se conserva la interacci\u00f3n electrost\u00e1tica entre los amino\u00e1cidos esenciales asp53-lys105 y glu-9-lys105. el \u00e1rea m\u00e1s flexible es la zona beta4-alfa4-beta5alfa5, caracter\u00edstica de los dominios receptores de las reguladoras de respuesta, aunque a diferencia de \u00e9stos se produce, en phob, el desplazamiento de la h\u00e9lice alfa4 a lo largo de la secuencia<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Estructura tridimensional del domini receptor del factor de transcripcio phob.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Estructura tridimensional del domini receptor del factor de transcripcio phob. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Mar\u00eda Sol\u00c1\u00a0 Vilarrubias <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Polit\u00e9cnica de catalunya<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Miquel Coll Capella<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  Antonio Subirana torrent <\/li>\n<li>francesc xavier Avil\u00e9s puigvert (vocal)<\/li>\n<li>l. Wanner barry (vocal)<\/li>\n<li>miquel Pons valles (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Mar\u00eda Sol\u00c1\u00a0 Vilarrubias Para ampliar el estudio de prote\u00ednas transductoras de se\u00f1al de dos componentes iniciado en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[160,115,15596,17],"tags":[18820,117,175849,175848,26322,19667],"class_list":["post-82802","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-bioquimica-molecular","category-politecnica-de-catalunya","category-quimica","tag-francesc-xavier-aviles-puigvert","tag-juan-antonio-subirana-torrent","tag-l-wanner-barry","tag-maria-sola-vilarrubias","tag-miquel-coll-capella","tag-miquel-pons-valles"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/82802","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=82802"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/82802\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=82802"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=82802"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=82802"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}