{"id":83137,"date":"2018-03-10T00:07:14","date_gmt":"2018-03-10T00:07:14","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/modificacion-de-las-propiedades-cataliticas-de-beta-clucosidasas-por-mutagenesis-dirigida\/"},"modified":"2018-03-10T00:07:14","modified_gmt":"2018-03-10T00:07:14","slug":"modificacion-de-las-propiedades-cataliticas-de-beta-clucosidasas-por-mutagenesis-dirigida","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/modificacion-de-las-propiedades-cataliticas-de-beta-clucosidasas-por-mutagenesis-dirigida\/","title":{"rendered":"\u00abmodificacion de las propiedades cataliticas de beta-clucosidasas por mutagenesis dirigida\u00bb"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Gracia Gonzalez Blasco <\/strong><\/h2>\n<p>Una importante utilidad de las t\u00e9cnicas de ingenieria de prote\u00ednas es la obtenci\u00f3n de nuevos tipos de enzimas especialmente dise\u00f1ados con vistas a su aplicaci\u00f3n industrial. En una prote\u00edna, la contribuci\u00f3n de residuos aminoac\u00eddicos individuales a su estabilidad y funcionalidad es un problema todav\u00eda sin resolver, sin embargo, la disponibilidad de genes clonados permite modificar las caracter\u00edsticas de los enzimas codificadas por ellos, mediante t\u00e9cnicas de mutag\u00e9nesis aleatoria y dirigida. En este trabajo de esta tesis doctoral se han modificado las propiedades de una beta-glucosidasa codificadapor el gen bg1a de paenibacillus plymyxa. Se ha puesto a punto dos m\u00e9todos de -mutag\u00e9nesis aleatoria para la modificaci\u00f3n de las propiedades de bg1a basados en la t\u00e9cnica de pcr. Mediante mutag\u00e9nesis aleatoria se han aislado y caracterizado seis mutaciones distintas que aumentan la resistencia t\u00e9rmica de bg1a. La combinaci\u00f3n de las distintas mutaciones en una misma mol\u00e9cula nos ha permitido obtener un mutante m\u00faltiple de bg1a con una vida media de tres minutos a 70 grados cent\u00edgrados, que puede calificarse como enzima termorresistente. Una de las mutaciones, t385a adem\u00e1s, produce un aumento de aproximadamente 20 veces la producci\u00f3n de enzima bg1a por escherichia coli.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>\u00abmodificacion de las propiedades cataliticas de beta-clucosidasas por mutagenesis dirigida\u00bb<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 \u00abmodificacion de las propiedades cataliticas de beta-clucosidasas por mutagenesis dirigida\u00bb <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Gracia Gonzalez Blasco <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Universitat de val\u00e9ncia (estudi general)<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/01\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Julio Polaina Molina<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: concepci\u00f3n Abad mazario <\/li>\n<li> Romey figuerola Antonio  ram\u00f3n (vocal)<\/li>\n<li>Miguel Vicente mu\u00f1oz (vocal)<\/li>\n<li>vicente Rubio zamora (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Gracia Gonzalez Blasco Una importante utilidad de las t\u00e9cnicas de ingenieria de prote\u00ednas es la obtenci\u00f3n de [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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