{"id":83139,"date":"2018-03-10T00:07:14","date_gmt":"2018-03-10T00:07:14","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-y-caracterizacion-de-genes-implicados-en-la-regulacion-de-la-h-atpasa-de-la-membrana-plasmatica-de-saccharomyces-cerevisiae\/"},"modified":"2018-03-10T00:07:14","modified_gmt":"2018-03-10T00:07:14","slug":"aislamiento-y-caracterizacion-de-genes-implicados-en-la-regulacion-de-la-h-atpasa-de-la-membrana-plasmatica-de-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/aislamiento-y-caracterizacion-de-genes-implicados-en-la-regulacion-de-la-h-atpasa-de-la-membrana-plasmatica-de-saccharomyces-cerevisiae\/","title":{"rendered":"Aislamiento y caracterizacion de genes implicados en la regulacion de la h+-atpasa de la membrana plasmatica de saccharomyces cerevisiae"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Natalia De La Fuente De Los Llanos <\/strong><\/h2>\n<p>En un trabajo anterior llevado a cabo en el laboratorio, se aislaron mutaciones en seis genes (apa2, apa3, apa4, apa5, apa6 y apa7) que afectaban a la regulaci\u00f3n de los niveles de la h+-atpasa de la membrana plasm\u00e1tica de saccharomyces cerevisiae.  el objetivo del presente trabajo fue la caracterizaci\u00f3n bioqu\u00edmica y molecular de estos genes. Los genes apa3, 4, 5 y 6, no pudieron ser clonados debido a la presencia en la genoteca de dos supresores no espec\u00edficos de alelo que se correspond\u00edan con mat1 y srk1. Los genes apa2 y apa7 pudieron ser identificados y resultaron ser rsp5 y yor137c, respectivamente.   yor137c codifica una prote\u00edna de membrana de funci\u00f3n desconocida. Su deleci\u00f3n no afecta a la cantidad de h+-atpasa presente en la membrana plasm\u00e1tica pero afecta al incremento de la vmax cuando activamos con glucosa.  rsp5 codifica una ubicuitina ligasa implicada en la ruta de degradaci\u00f3n proteol\u00edtica ubicuitina-proteasoma. Mutaciones en dicho gen afectan al descenso que se produce en la km en presencia de glucosa. Su estudio nos llev\u00f3 a postular la existencia de una hipot\u00e9tica prote\u00edna negativa del proceso de activaci\u00f3n de la h+-atpasa. En el intento de determinar la posible naturaleza de dicha prote\u00edna, mediante el empleo de diversas estrategias, encontramos que la ruta wsc2-rho1-pkc1-bck1-mkk1\/2-mpk1, el factor de transcripci\u00f3n hal7\/yap6 y dos prote\u00ednas de funci\u00f3n desconocida, codificadas por los genes yjr134c e ymr102c, deb\u00edan estar implicados en la regulaci\u00f3n de la expresi\u00f3n de genes que intervienen en la activaci\u00f3n de la atpasa.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento y caracterizacion de genes implicados en la regulacion de la h+-atpasa de la membrana plasmatica de saccharomyces cerevisiae<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento y caracterizacion de genes implicados en la regulacion de la h+-atpasa de la membrana plasmatica de saccharomyces cerevisiae <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Natalia De La Fuente De Los Llanos <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 27\/01\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Francisco Portillo Perez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Sillero repullo <\/li>\n<li>i\u00f1igo Fernandez de carrinda (vocal)<\/li>\n<li>ram\u00f3n Serrano salom (vocal)<\/li>\n<li>alonso Rodriguez navarro (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Natalia De La Fuente De Los Llanos En un trabajo anterior llevado a cabo en el laboratorio, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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