{"id":8317,"date":"1995-01-01T00:00:00","date_gmt":"1995-01-01T00:00:00","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/1995\/01\/01\/preparacion-de-un-material-de-referencia-europeo-de-alfa-amilasa-pancreatica-humana\/"},"modified":"1995-01-01T00:00:00","modified_gmt":"1995-01-01T00:00:00","slug":"preparacion-de-un-material-de-referencia-europeo-de-alfa-amilasa-pancreatica-humana","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/preparacion-de-un-material-de-referencia-europeo-de-alfa-amilasa-pancreatica-humana\/","title":{"rendered":"Preparacion de un material de referencia europeo de alfa-amilasa pancreatica humana"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Gemma Gubern Olivella <\/strong><\/h2>\n<p>El objetivo del trabajo era la preparacion y certificacion de un material de referencia de la amilasa incluido en el programa del community bureau of reference. Este material permitira armonizar las mediciones de la concentracion catalitica de la amilasa en los laboratorios europeos. Se purificaron 30 mg de amilasa con una actividad especifica de 52,9 ku\/g a partir de pancreas humano. La masa molecular relativa de la enzima purificada era de 57800 con un punto isoelectrico de 7,1. Se selecciono una matriz en la que la enzima fuese estable. Se preparo el material de referencia diluyendolo en la matriz que contenia tampon pipes 25 mmol\/l, ph 7,0, nacl 50 mmol\/l, edta 0,5 mmol\/l, cacl2 1,5 mmol\/l y albumina humana 30 g\/l y se liofilizo.  el material de referencia era homogeneo y conmutable con el enzima presente en suero humano. La perdida anual de actividad catalitica se estimo en 0% a -20 grados c. Al no existir metodo de referencia, se selecciono el metodo que usa como sustrato 2-cloro-4-nitrofenil-maltotriosido para la asignacion del valor al material de referencia.  se optimizo este metodo de medida. El valor certificado a 37 grados c de concentracion catalitica obtenido en un estudio interlaboratorio corresponde a 555 u\/l +- o u\/l o 9,25  kat\/l+- o,17  kat\/l.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Preparacion de un material de referencia europeo de alfa-amilasa pancreatica humana<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Preparacion de un material de referencia europeo de alfa-amilasa pancreatica humana <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Gemma Gubern Olivella <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Aut\u00f3noma de barcelona<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 01\/01\/1995<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li> Gella Tomas Francisco Javier<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Francesc Gonz\u00e1lez Sastre <\/li>\n<li>Joan Guinovart Cirera (vocal)<\/li>\n<li>Jos\u00e9 Carreras Barn\u00e9s (vocal)<\/li>\n<li>Rosa Segura Cardona (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Gemma Gubern Olivella El objetivo del trabajo era la preparacion y certificacion de un material de referencia [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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