{"id":83883,"date":"2018-03-10T00:08:03","date_gmt":"2018-03-10T00:08:03","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/aislamiento-y-caracterizacion-de-genes-de-biosintesis-del-antibiotico-glicopeptidico-vancomicina\/"},"modified":"2018-03-10T00:08:03","modified_gmt":"2018-03-10T00:08:03","slug":"aislamiento-y-caracterizacion-de-genes-de-biosintesis-del-antibiotico-glicopeptidico-vancomicina","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/aislamiento-y-caracterizacion-de-genes-de-biosintesis-del-antibiotico-glicopeptidico-vancomicina\/","title":{"rendered":"Aislamiento y caracterizacion de genes de biosintesis del antibiotico glicopeptidico vancomicina"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Jose Garcia-bernardo Junquera <\/strong><\/h2>\n<p>En esta tesis doctoral se ha llevado a cabo el estudio de la ruta de bios\u00edntesis del antibi\u00f3tico glicopept\u00eddico vancomicina. La importancia de este antibi\u00f3tico se centra en su actividad antibi\u00f3tica frente a bacterias grampositivas frente a las que otros antibi\u00f3ticos de uso tradicional, como los antibi\u00f3ticos beta-lact\u00e1micos, no resultan efectivos. Para llevar a cabo este estudio se procedi\u00f3 a la construcci\u00f3n de una genoteca del adn cromos\u00f3mico del microorganismo productor de vancomicina, amycolatopsis orientalis, utilizando como vector de clonaci\u00f3n el c\u00f3smido bifuncional para streptomyces y escherichia coli pkc505. El tama\u00f1o medio de los insertos del adn cromos\u00f3mico en dicho c\u00f3smido estaba comprendido entre 18 y 30 kb. Se generaron 3300 clones en la genoteca, por lo que teniendo en cuenta que el tama\u00f1o del genoma de este microorganismo est\u00e1 proximo a las 10 mb, la representatividad lograda en la genoteca ronda el 99%.  para la localizaci\u00f3n de los genes implicados en la ruta de bios\u00edntesis del antibi\u00f3tico glicopept\u00eddico vancomicina, se procedi\u00f3 al an\u00e1lisis de la genoteca mediante hibridaci\u00f3n in situ. Para ello se utilizaron tres tipos de sondas, tanto hom\u00f3logas como heter\u00f3logas. En primer lugar se utiliz\u00f3 una sonda de genes de bios\u00edntesis de deoxiaz\u00facares de antibi\u00f3ticos; en concreto, los genes mtmd y mtme, implicados en las dos primeras reacciones de la ruta de bios\u00edntesis del antibi\u00f3tico antitumoral mitramicina. Las reacciones llevadas a cabo por los enzimas codificados por estos dos genes son comunes a la mayor\u00eda de las rutas de bios\u00edntesis de deoxiaz\u00facares presentes en antibi\u00f3ticos. La presencia del deoxiaz\u00facar l-vancosamina en la mol\u00e9cula de vancomicina justificaba por tanto la utilizaci\u00f3n de este tipo de sondas.  la segunda sonda utilizada estaba implicada en sistemas de tipo abc, implicados en exportaci\u00f3n y resistencia a antibi\u00f3ticos en los microorganismos productores. Esta sonda se amplific<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Aislamiento y caracterizacion de genes de biosintesis del antibiotico glicopeptidico vancomicina<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Aislamiento y caracterizacion de genes de biosintesis del antibiotico glicopeptidico vancomicina <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Jose Garcia-bernardo Junquera <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Oviedo<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 24\/03\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Jos\u00e9 Antonio Salas Fernandez<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Juan  evaristo Suarez fernandez <\/li>\n<li>Mar\u00eda enriqueta Arias fern\u00e1ndez (vocal)<\/li>\n<li>ram\u00f3n Santamar\u00eda s\u00e1nchez (vocal)<\/li>\n<li>Francisco Parra fernandez (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Jose Garcia-bernardo Junquera En esta tesis doctoral se ha llevado a cabo el estudio de la ruta [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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