{"id":84532,"date":"2018-03-10T00:08:51","date_gmt":"2018-03-10T00:08:51","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/analisis-mutacional-del-dominio-de-actividad-nucleasica-5a-3ade-la-dna-polimerasa-i-de-streptococcus-pneumoniae\/"},"modified":"2018-03-10T00:08:51","modified_gmt":"2018-03-10T00:08:51","slug":"analisis-mutacional-del-dominio-de-actividad-nucleasica-5a-3ade-la-dna-polimerasa-i-de-streptococcus-pneumoniae","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/quimica\/analisis-mutacional-del-dominio-de-actividad-nucleasica-5a-3ade-la-dna-polimerasa-i-de-streptococcus-pneumoniae\/","title":{"rendered":"Analisis mutacional del dominio de actividad nucleasica 5\u00c2\u00bf-3\u00c2\u00bfde la dna polimerasa i de streptococcus pneumoniae."},"content":{"rendered":"<h2>Tesis doctoral de <strong> Monica Amblar Esteban <\/strong><\/h2>\n<p>El trabajo de esta tesis doctoral se ha centrado en la caracterizaci\u00f3n de la actividad 5\u00c2\u00bfde la dna polimerasa i de streptococcus pneumoniae. Mediante el an\u00e1lisis mutacional del dominio de actividad nucleasa y la determinaci\u00f3n de las actividades enzim\u00e1ticas de los derivados mutacionales, se ha establecido que mutaciones introducidas en los residuos d10,d190 y e114 afectan s\u00f3lo las actividades relacionadas con el dominio nucleasa. Los estudios realizados han generado resultados, que indican la funci\u00f3n de dichos residuos y apoyan un modelo catalitico que requiere la uni\u00f3n de dos metales (me1 y me2). el residuo d190 parece estar implicado en el evento catal\u00edtico interaccionado con el metal me2 requerido para la actividad nucle\u00e1sica. El residuo e114 estar\u00eda implicado en la uni\u00f3n al dna sustrato y proximo al sitio del me1. el residuo d10 estar\u00eda localizado en las proximidades del sitio me1, sin interaccionar directamente con el metal, y siendo requerido para la actividad catal\u00edtica. El derivado mutacional d10a, carente de actividad nucle\u00e1sica, ha sido analizado para su utilizaci\u00f3n en tecnicas de secuenciaci\u00f3n de dna manual y autom\u00e1tica. Se ha comprobado que dicha prote\u00edna es capaz de elongar oligonucle\u00f3tidos marcados fluorescentemente que emiten luz en el reango de infrarojo y de incorporar nucle\u00f3tidos marcados con digoxigenina(marcaje interno) dando un patr\u00f3n de bandas homogeneo, caracter\u00edsticas que no presentan otras polimerasas, como las del bacteri\u00f3fago t7 y la de thermus aquaticus, utilizadas rutinariamente en t\u00e9cnicas de secuenciaci\u00f3n.<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Analisis mutacional del dominio de actividad nucleasica 5\u00c2\u00bf-3\u00c2\u00bfde la dna polimerasa i de streptococcus pneumoniae.<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Analisis mutacional del dominio de actividad nucleasica 5\u00c2\u00bf-3\u00c2\u00bfde la dna polimerasa i de streptococcus pneumoniae. <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Monica Amblar Esteban <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Complutense de Madrid<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 16\/05\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Paloma L\u00f3pez Garc\u00eda<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: Antonio Tormo garrido <\/li>\n<li>ramon Diaz orejas (vocal)<\/li>\n<li>Luis Blanco d\u00e1vila (vocal)<\/li>\n<li> Martin sanchez Mar\u00eda  Jes\u00fas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Monica Amblar Esteban El trabajo de esta tesis doctoral se ha centrado en la caracterizaci\u00f3n de la [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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