{"id":85297,"date":"2018-03-10T00:09:45","date_gmt":"2018-03-10T00:09:45","guid":{"rendered":"https:\/\/www.deberes.net\/tesis\/sin-categoria\/arquitectura-y-ensamblaje-del-relaxosoma-en-el-plasmido-conjugativo-r388\/"},"modified":"2018-03-10T00:09:45","modified_gmt":"2018-03-10T00:09:45","slug":"arquitectura-y-ensamblaje-del-relaxosoma-en-el-plasmido-conjugativo-r388","status":"publish","type":"post","link":"https:\/\/www.deberes.net\/tesis\/ciencias-de-la-vida\/arquitectura-y-ensamblaje-del-relaxosoma-en-el-plasmido-conjugativo-r388\/","title":{"rendered":"Arquitectura y ensamblaje del relaxosoma en el plasmido conjugativo r388"},"content":{"rendered":"<h2>Tesis doctoral de <strong> Gabriel Moncalian Montes <\/strong><\/h2>\n<p>El relaxosoma es el complejo nucleoprote\u00edco necesario para un efectivo procesamiento del dna en la conjugaci\u00f3nbacteriana, present\u00e1ndose en esta memoria de tesis doctoral un estudio de los componentes del relaxosoma del pl\u00e1smido r388, de la prote\u00edna acopladora trwb y de la interacci\u00f3n entre ambos.  en primer lugar se estudiaron las prote\u00ednas componentes del relaxosoma de r388 comenzando por la prote\u00edna accesoria trwa. A partir de la construcci\u00f3n de un pl\u00e1smido sobreproductor de trwa se purific\u00f3 para su estudio bioqu\u00edmico, comprb\u00e1ndose su uni\u00f3 a dos sitios (sbaa y sbab) en el orit de r388 que activa la actividad de corte de rrwc y reprime la transcripci\u00f3n del oper\u00f3n trwabc. Un posterior an\u00e1lisis por medio de mutantes sirvi\u00f3 para la determianci\u00f3n de los amino\u00e1cidos de trwa implicados en el reconocimiento de secuencias espec\u00edficas en el ort.  a continuaci\u00f3n y para determinar cual era el sitio de uni\u00f3nde trwc al orit se realizaron ensayos de footprinting de dna con dms y, por medio del tratamiento conpermanganato, se estudi\u00f3 la modificaic\u00f3n sufrida por el dna al unirse trwc. Estos estudios de footprinting se realizaron tambi\u00e9n en presencia de otras prote\u00ednas como trwa o ihf tanto en vitro como in vivo.  adem\u00e1s, a pesar de nohaberse observado ning\u00fan efecto in vivo de ihf sobre la conjugaci\u00f3n de r388, se comprob\u00f3 que ihf se un\u00edan al orit de r388, como suger\u00eda la presencia de una secuencia consenso para dicha uni\u00f3n. Adem\u00e1s, se estudiaron las posibles implicaciones de esa uni\u00f3n.  por otro lado, la obtenci\u00f3n por nuestro grupo de una prote\u00edna con la parte soluble de trwb (trwban70) facilitaba en gran medida su purificaci\u00f3n y estudio. Partiendo de esta prote\u00edna y de la porte\u00edna mutante trwb(k136t)an70 se estudi\u00f3 la uni\u00f3n a atp y su hidr\u00f3lisis, la uni\u00f3n a dna y la uni\u00f3n a otras prote\u00ednas. Estos datos nos dieron una idea de c\u00f3mo es la interacci\u00f3n de trwb con el relaxosoma. Adem\u00e1s la prote\u00edna trwban<\/p>\n<p>&nbsp;<\/p>\n<h3>Datos acad\u00e9micos de la tesis doctoral \u00ab<strong>Arquitectura y ensamblaje del relaxosoma en el plasmido conjugativo r388<\/strong>\u00ab<\/h3>\n<ul>\n<li><strong>T\u00edtulo de la tesis:<\/strong>\u00a0 Arquitectura y ensamblaje del relaxosoma en el plasmido conjugativo r388 <\/li>\n<li><strong>Autor:<\/strong>\u00a0 Gabriel Moncalian Montes <\/li>\n<li><strong>Universidad:<\/strong>\u00a0 Cantabria<\/li>\n<li><strong>Fecha de lectura de la tesis:<\/strong>\u00a0 26\/06\/2000<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3>Direcci\u00f3n y tribunal<\/h3>\n<ul>\n<li><strong>Director de la tesis<\/strong>\n<ul>\n<li>Fernando De La Cruz Calahorra<\/li>\n<\/ul>\n<\/li>\n<li><strong>Tribunal<\/strong>\n<ul>\n<li>Presidente del tribunal: margarita Salas falgueras <\/li>\n<li>Francisco Murillo  araujo (vocal)<\/li>\n<li>ramon Diaz orejas (vocal)<\/li>\n<li>Jos\u00e9 Manuel Guis\u00e1n seijas (vocal)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tesis doctoral de Gabriel Moncalian Montes El relaxosoma es el complejo nucleoprote\u00edco necesario para un efectivo procesamiento del dna en [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-gradient":""}},"footnotes":""},"categories":[212,10518,35,962,2820],"tags":[10595,8465,179684,24291,15196,6062],"class_list":["post-85297","post","type-post","status-publish","format-standard","hentry","category-biologia-humana","category-cantabria","category-ciencias-de-la-vida","category-genetica","category-genetica-humana","tag-fernando-de-la-cruz-calahorra","tag-francisco-murillo-araujo","tag-gabriel-moncalian-montes","tag-jose-manuel-guisan-seijas","tag-margarita-salas-falgueras","tag-ramon-diaz-orejas"],"_links":{"self":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/85297","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/comments?post=85297"}],"version-history":[{"count":0,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/posts\/85297\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/media?parent=85297"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/categories?post=85297"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.deberes.net\/tesis\/wp-json\/wp\/v2\/tags?post=85297"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}